StemRD  PSGro 人iPS細(xì)胞和胚胎干細(xì)胞無(wú)血清培養(yǎng)基

產(chǎn)品英文名稱：StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium

產(chǎn)品中文名稱：StemRD人iPS細(xì)胞和胚胎干細(xì)胞無(wú)血清培養(yǎng)基,無(wú)異源級(jí)別

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干細(xì)胞無(wú)血清培養(yǎng)基 500mL/套

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干細(xì)胞無(wú)血清培養(yǎng)基產(chǎn)品特點(diǎn)：

★ Serum-Free 無(wú)血清成分；

★ Xeno-Free 無(wú)異種蛋白；

★ Chemically-Defined 化學(xué)成分明確；

★ Feeder-Independent 無(wú)需滋養(yǎng)層細(xì)胞；

★ Supports human iPSCs/ESCs 用于人iPS細(xì)胞和胚胎干細(xì)胞生長(zhǎng)；

★ Performs Comparably to mTeSR 1 效能與mTeSR 1培養(yǎng)基同等。

Characteristic Morphology Human ESCs(A,B); iPSCs(C,D) in PSGro-Free®

PSGro-Free 是stemRD開(kāi)發(fā)的一款針對(duì)人類胚胎干細(xì)胞（ESCs）和多能誘導(dǎo)干細(xì)胞（iPSCs）的生長(zhǎng)維護(hù)胚胎干細(xì)胞無(wú)血清培養(yǎng)基。我們生產(chǎn)質(zhì)量可靠的生長(zhǎng)因子，并擁有來(lái)自SystemBiosciences的優(yōu)秀iPS研究專家。PSGro-Free的所有添加組分都為化學(xué)合成或已純化的重組蛋白，并且決不含有任何非人源的異種成分。

PSGro-Free is a growth medium designed for humaninduced pluripotent stem cells ( iPSCs ) and humanembryonic stem cells (ESCs). It was developed atStemRD, a premier producer of growth factors, incollaboration with iPS experts at System Biosciences.

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司        華雅干細(xì)胞-：

提供產(chǎn)品：華雅干細(xì)胞致力于為中國(guó)干細(xì)胞領(lǐng)域的干細(xì)胞科學(xué)研究、臍帶血/造血干細(xì)胞/臍帶/脂肪/胎盤干細(xì)胞庫(kù)、干細(xì)胞臨床轉(zhuǎn)化醫(yī)學(xué)、干細(xì)胞美容及干細(xì)胞抗衰老、免疫細(xì)胞治療、T細(xì)胞治療、臨床干細(xì)胞治療等再生醫(yī)學(xué)和個(gè)體化治療產(chǎn)業(yè)鏈的相關(guān)客戶提供：MSC間充質(zhì)干細(xì)胞培養(yǎng)基、iPS/ES胚胎干細(xì)胞培養(yǎng)基、血清替代物、羊水培養(yǎng)基、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、BioMarker 流式檢測(cè)抗體、支原體檢測(cè)試劑盒、內(nèi)毒素檢測(cè)試劑盒、干細(xì)胞存儲(chǔ)細(xì)胞凍存袋、細(xì)胞培養(yǎng)瓶、USP級(jí)DMSO二甲基亞砜細(xì)胞凍存液、細(xì)胞培養(yǎng)相關(guān)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on improving the quality of Stem Cells專注干細(xì)胞研究，提升干細(xì)胞品質(zhì)”為己任，我們與客戶攜手為中國(guó)干細(xì)胞產(chǎn)業(yè)的發(fā)展而前行。

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干細(xì)胞無(wú)血清培養(yǎng)基 產(chǎn)品英文描述如下：

PSGro-case is designed for human induced pluripotent stem cells (iPSCs) and human embryonic stem cells (ESCs). It is serum-free and chemically-defined as all of its ingredients are synthetic chemicals or purified recombinant proteins. PSGro-case supports feeder-free growth and maintenance of human iPSCs or human ESCs on extra-cellular matrix proteins (e.g., BD Matrigel?). Its performance is comparable to or better than the market-leading mTeSR?1 or E8 in growth rate, characteristic morphology, pluoripotent markers, normal karyotype, and differentiation to different germ layers. The robustness and stability of PSGro-case enable seeding cells over the weekend without changing medium, so you have the weekend Free.

StemRD PGroF-500 PSGro-case, Human iPSC/ESC Growth Medium 產(chǎn)品組分：

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司        華雅干細(xì)胞-：

提供產(chǎn)品：華雅干細(xì)胞致力于為中國(guó)干細(xì)胞領(lǐng)域的干細(xì)胞科學(xué)研究、臍帶血/造血干細(xì)胞/臍帶/脂肪/胎盤干細(xì)胞庫(kù)、干細(xì)胞臨床轉(zhuǎn)化醫(yī)學(xué)、干細(xì)胞美容及干細(xì)胞抗衰老、免疫細(xì)胞治療、T細(xì)胞治療、臨床干細(xì)胞治療等再生醫(yī)學(xué)和個(gè)體化治療產(chǎn)業(yè)鏈的相關(guān)客戶提供：MSC間充質(zhì)干細(xì)胞培養(yǎng)基、iPS/ES胚胎干細(xì)胞培養(yǎng)基、血清替代物、羊水培養(yǎng)基、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、BioMarker 流式檢測(cè)抗體、支原體檢測(cè)試劑盒、內(nèi)毒素檢測(cè)試劑盒、干細(xì)胞存儲(chǔ)細(xì)胞凍存袋、細(xì)胞培養(yǎng)瓶、USP級(jí)DMSO二甲基亞砜細(xì)胞凍存液、細(xì)胞培養(yǎng)相關(guān)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on improving the quality of Stem Cells專注干細(xì)胞研究，提升干細(xì)胞品質(zhì)”為己任，我們與客戶攜手為中國(guó)干細(xì)胞產(chǎn)業(yè)的發(fā)展而前行。

Content and Storage: Components  Size  Storage   Shelf Life

Basal Medium (on gel ice)   450mL    2 to 8°C. Keep from light   6 months

10x Supplement (dry ice)  50mL    -20°C or below. Keep from light  6 months      

Additional key reagents required or suggested

Extracellular matrix proteins, such as Matrigel  (BD, cat# 354277) or equivalent

Accutase (Millipore, SCR005) or Collagenase IV (Invitrogen, 17104) or equivalent

ROCK inhibitor Y-27632 (StemRD, Y-01/-05/-25) or Thiazovivin (StemRD, Thia-01/-05/-25)

Medium Preparation

1. Thaw PSGro-case Supplement overnight at 4°C. If desired, thawed Supplement can be aliquoted and stored at -20°C or below, but further freeze-thaw should be avoided.

2. To make PSGro-case complete medium, add 1 part of Supplement to 9 parts of Basal Medium.

3. Antibiotics can be added to the complete medium if so desired.

4. Once made, PSGro-case  complete medium is stable for 2 weeks when stored in dark at 2 to 8°C.

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司        華雅干細(xì)胞-：

提供產(chǎn)品：華雅干細(xì)胞致力于為中國(guó)干細(xì)胞領(lǐng)域的干細(xì)胞科學(xué)研究、臍帶血/造血干細(xì)胞/臍帶/脂肪/胎盤干細(xì)胞庫(kù)、干細(xì)胞臨床轉(zhuǎn)化醫(yī)學(xué)、干細(xì)胞美容及干細(xì)胞抗衰老、免疫細(xì)胞治療、T細(xì)胞治療、臨床干細(xì)胞治療等再生醫(yī)學(xué)和個(gè)體化治療產(chǎn)業(yè)鏈的相關(guān)客戶提供：MSC間充質(zhì)干細(xì)胞培養(yǎng)基、iPS/ES胚胎干細(xì)胞培養(yǎng)基、血清替代物、羊水培養(yǎng)基、基底膜基質(zhì)膠細(xì)胞支架、干細(xì)胞生長(zhǎng)分化調(diào)控因子、BioMarker 流式檢測(cè)抗體、支原體檢測(cè)試劑盒、內(nèi)毒素檢測(cè)試劑盒、干細(xì)胞存儲(chǔ)細(xì)胞凍存袋、細(xì)胞培養(yǎng)瓶、USP級(jí)DMSO二甲基亞砜細(xì)胞凍存液、細(xì)胞培養(yǎng)相關(guān)試劑耗材儀器等種類豐富的產(chǎn)品，以“Focus on improving the quality of Stem Cells專注干細(xì)胞研究，提升干細(xì)胞品質(zhì)”為己任，我們與客戶攜手為中國(guó)干細(xì)胞產(chǎn)業(yè)的發(fā)展而前行。

StemRD PGroF-500 PSGro-case, Human iPSC/ESC growth medium 人iPSC/ESC胚胎干細(xì)胞無(wú)血清培養(yǎng)基 評(píng)價(jià)或評(píng)論

Coating plates with extracellular matrix proteins, such as Matrigel

Refer to manufacturers’ instructions. For BD Matrigel?, the following procedure can be followed:

1. Thaw Matrigel on ice. Dilute Matrigel 1:50 with pre‐chilled DMEM medium.

2. Immediay use the diluted Matrigel? solution to coat tissue culture plates. For a 6‐well plate, use 1 mL of diluted Matrigel? solution per well, and swirl the plate to spread the solution evenly.

3. Let the plate stand for 1 h at 37°C or overnight at 4°C.

Adaptation of growing feeder-free cells to PSGro-case

Human iPSC or ESCs that have been grown in most commonly-used hiPSC/ESC media can be easily adapted into PSGro-case. One round of 1:1 dilution of serum-containing or serum-free medium with PSGro-case is generally sufficient. However, for certain media and cell lines, a longer and more graduate adaptation may be required.

Adaptation of growing feeder-dependent cells to PSGro-case

1. Dissociate cells with Accutase, Collagenase IV or equivalent.

2. Add the previously-used medium and gently scrape off cells as aggregates. The ideal aggregate size is similar to other media and single cells from excessive pipetting may lower plating efficiency.

3. Transfer the suspension with cell aggregates into a 15 ml tube.

4. Centrifuge at 200 x g for 5 minutes at room temperature.

5. Discard Matrigel? solution, immediay add PSGro-case (e.g., 2 mL/well for a 6-well plate).

6. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.

7. Transfer the cell aggregates in the previously-used medium to the wells with PSGro-case. Mix. Gently.

8. Culture the cells at 37°C, with 5% CO2 and 95% humidity.

9. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case (no need to change over the weekend). If cells are seeded on other days during the week, change next day.

重慶市華雅干細(xì)胞技術(shù)有限公司主營(yíng)：干細(xì)胞、細(xì)胞凍存、細(xì)胞治療、試劑耗材 相關(guān)板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近五年的努力推廣，重慶市華雅干細(xì)胞技術(shù)有限公司在全中國(guó)代理的BD科研產(chǎn)品、OriGen干細(xì)胞凍存袋等產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司     華雅干細(xì)胞-：

Recovery of frozen cells in PSGro-case

1. Quickly thaw hiPSCs or hESCs in a 37°C water bath, transfer the contents to a 15 mL tube. Add 10 mL of the medium previously-used for the cells drop-wise to the tube with gentle mixing.

2. Centrifuge cells at 200 x g for 5 minutes at room temperature.

3. Discard Matrigel solution, immediay add PSGro-case  (e.g., 2 mL/well for a 6-well plate).

4. After centrifugation, discard the supernatant, gently resuspend the pellet with 2 mL of the previously-used medium. Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.

5. Transfer the cell aggregates in the previously-used medium to the plate with PSGro-case . Mix gently.

6. Culture the cells at 37°C, with 5% CO2 and 95% humidity.

7. Change medium to 4 mL PSGro-case next day.

重慶市華雅干細(xì)胞技術(shù)有限公司主營(yíng)：干細(xì)胞、細(xì)胞凍存、細(xì)胞治療、試劑耗材 相關(guān)板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近五年的努力推廣，重慶市華雅干細(xì)胞技術(shù)有限公司在全中國(guó)代理的BD科研產(chǎn)品、OriGen干細(xì)胞凍存袋等產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司     華雅干細(xì)胞-：

Passaging cells in PSGro-case  under feeder-free condition

1. Look under microscope to identify regions of differentiation. Mark the differentiated colonies using lens marker on the bottom of the plate (e.g., 6-well plate)

2. Remove regions of differentiation by scraping with a pipette tip or by aspiration.

3. Aspirate medium from the cell culture and rinse with PBS.

4. Add 0.5 mL per well of Accutase or Collagenase IV. Let stand at room temp for 1 – 2 min.

5. Remove Accutase, and gently rinse 2 – 3 times with 2 mL PSGro-case to remove remaining

enzymes.

6. Add 2 mL/well PSGro-case and scrape colonies off with a cell scraper.

7. Transfer the detached cell aggregates to a 15 mL conical tube and rinse the well with an additional

2 mL PSGro-case to collect any remaining aggregates.

8. Centrifuge at 200 x g for 5 minutes at room temperature.

9. Aspirate the supernatant. Resuspend pellet in 4 mL PSGro-case by pipetting gently.

Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.

10. Plate the cell aggregates with PSGro-case onto a new plate coated with Matrigel.

11. Culture the cells at 37°C, with 5% CO2 and 95% humidity. Change medium daily.

12. If cells are seeded on Friday, change medium on Monday to 4 mL PSGro-case (no need to change over the weekend). If cells are seeded on other days during the week, change next day.

Note: If colonies are at an optimal density, cells can be split every 5 ‐ 7 days using 1:5 to 1:10 splits.

Cryopreservation of cells in PSGro-case

1. Prepare freezing medium which contains 90% PSGro-case and 10% DMSO.

Note: Adding ROCK inhibitor (Y-27632 or Thiazovivin) to the medium is shown to increase the survival and plating efficiency.

2. Perform steps 1 - 8 from Passaging cells in PSGro-case under feeder-free condition

3. Gently aspirate the supernatant taking care to keep the cell pellet intact.

4. Gently resuspend the pellet in freezing medium, taking care to leave the cell aggregates larger than would normally be done for passaging.

5. Transfer 1 mL of cell aggregates in freezing medium into each labeled cryovial.

6. Place vials into an isopropanol freezing container and place the container at ‐80°C overnight.

7. Transfer to a liquid nitrogen tank the next day for long-term storage.

重慶市華雅干細(xì)胞技術(shù)有限公司主營(yíng)：干細(xì)胞、細(xì)胞凍存、細(xì)胞治療、試劑耗材 相關(guān)板塊的產(chǎn)品，以“傳遞科學(xué)價(jià)值，服務(wù)科學(xué)研究”為宗旨，經(jīng)過(guò)近五年的努力推廣，重慶市華雅干細(xì)胞技術(shù)有限公司在全中國(guó)代理的BD科研產(chǎn)品、OriGen干細(xì)胞凍存袋等產(chǎn)品已經(jīng)成功銷往：上海市、北京市、重慶市、天津市、廣東省的廣州市、深圳市、珠海市、江西省的南昌市、四川省的成都市、綿陽(yáng)、浙江省的杭州市、寧波、溫州、江蘇省的南京市、蘇州、無(wú)錫、泰州、湖南省的長(zhǎng)沙、陜西省的西安市、楊凌、甘肅省的蘭州、寧夏的銀川、新疆的烏魯木齊、廣西省的南寧市、山東省的濟(jì)南、青島、東北三省 遼寧省的沈陽(yáng)市、大連市、吉林省的長(zhǎng)春市、黑龍江省的哈爾濱市等中國(guó)絕大部分省市和自治區(qū)。

歡迎您致電 重慶市華雅干細(xì)胞技術(shù)有限公司     華雅干細(xì)胞-：