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KitContents
Please refer to complete instructions manual supplied with the product

SpeciesReactivity
Human

Form
Stds=0-100 pg/ml; Sample=25 ul; Sensitivity= 0.17 pg/ml; 75 min assay; Quantitative; Competitive assay; 96 tests

Samples
Serum. Testosterone kit has been used in mouse/rat serum and plasma (see published references). Other fluids may be adapted

SpCrossreactivity
The following compounds were tested for cross-reactivity with the free Testosterone ELISA kit with testosterone cross-reacting at 100%: Testosterone (100%), 5a-DHT (5.2%), Androstenedione (1.4%), Androstanediol (0.8%), Progesterone (0.5%), Androsterone (0.1%). The following steroids were tested but cross-reacted at less than 0.1%: Aldosterone, Andrenosterone, Cholesterol, Corticosterone, Dehydroepiandrosterone, Dehydroepiandrosterone Sulfate, Epiandrosterone, 17ß-Estradiol, Estriol and Pregnenolone. Testosterone kit has been designed and tested for human serum samples. It may be optimized for other biological fluids. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in all species, this kit should work in most species as long as the sample cocn is within the range of this kit. This kit has been used in mouse and rat samples (see publications).

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.