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Species
Mouse

SpeciesReactivity
Mouse

Form
Stds= 0-4000 ng/ml; Sample: 100 ul (1:100); Sensitivity; 125 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified SSA Antigen Coated ( 8 wells x 12 strips) 5711 2. Mouse anti-SSA Ig?s std A (0 ug/ml), 0.650 ml 5712 3. Mouse anti-SSA Ig?s std B (0.25 ug/ml),0.650 ml 5713 4. Mouse anti-SSA Ig?s std C (0.5 ug/ml), 0.650 ml 5714 5. Mouse anti-SSA Ig?s std D (1 ug/ml), 0.650 ml 5715 6. Mouse anti-SSA Ig?s std E (2 ug/ml), 0.650 ml 5716 7. Mouse anti-SSA Ig?s std F (4 ug/ml), 0.650 ml 5717 8. Goat Anti-mouse IgG (H+L)-HRP conjugate, 100X 0.120 ml 5718 9. Sample Diluent (10x), 10 ml SD-10 10. Wash Solution (100X), 10 ml WB-100 11. TMB Substrate, 12 ml 80091 12. Stop solution, 12 ml 80101 13. Complete Instruction Manual M-5710

SpCrossreactivity
The SS-A antigen, used for coating ELISA plates, has been purified from bovine spleen and/or thymus cytosol through a combination of immunoaffinity chromatography and other biochemical techniques. The purified preparation does not contain any detectable SS-B, Sm, nRNP, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit will measures anti-SSA toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.