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SpeciesReactivity
Mouse

Form
Stds= 0-75 ng/ml; Sample: 100 ul (1:100); Sensitivity; 2 ng/ml; 75 min assay; Quantitative; Ag-based Ab assay, 96 tests

Samples
Serum. Plasma, Culture medium and other fluids may be adapted

KitContents
1. Purified SSB Antigen Coated (8 wells x 12 strips) 5811 2. Mouse anti-SSB Ig?s std A (0 ng/ml), 0.650 ml 5812 3. Mouse anti-SSB Ig?s std B (4.68 ng/ml), 0.650 ml 5813 4. Mouse anti-SSB Ig?s std C (9.37 ng/ml), 0.650 ml 5814 5. Mouse anti-SSB Ig?s std D (18.75 ng/ml),0.650 ml 5815 6. Mouse anti-SSB Ig?s std E (37.5 ng/ml), 0.650 ml 5 8 1 6 7. Mouse anti-SSB Ig?s std F (75 ng/ml), 0.650 ml 5 8 1 7 8. Goat Anti-Mouse IgG HRP conjugate 100X (H+L), 9. 0.120 ml 5818 10. Sample Diluent (10x), 10 ml SD-10 11. Wash Solution (100X), 10 ml 80081 12. TMB Substrate, 12 ml 80091 13. Stop solution,12 ml 80101 14. Complete Instruction Manual M-5810

SpCrossreactivity
The SSB/la antigen, used for coating ELISA plates, has been purified from calf and rabbit thymus cytosol through a combination of immunoaffinity chromatography and other biochemical techniques. The purified preparation does not contain any detectable SS-A, Sm, nRNP, Scl-70, and Jo-1 antigens by sensitive ELISA technique. This kit measures anti-SSB toal Ig?s (IgG, IgA, and IgM) as the conjugate supplied in the kit is anti-mouse IgG (H+L)-HRP conjugate. It is possible to use isotype specific anti-mouse Ig, such as anti-mouse IgM or anti-mouse IgG1-HRP, to measure isotype specific antibodies.

MSDS
TMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates

For research use only, not for diagnostic or therapeutic use.