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        1. 上海易佰聚經(jīng)貿(mào)有限公司
          中級(jí)會(huì)員 | 第15年

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          當(dāng)前位置:上海易佰聚經(jīng)貿(mào)有限公司>>生化試劑>>瓊脂糖>> 20665PIERCE,DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL

          PIERCE,DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL

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          產(chǎn)品型號(hào)20665

          品       牌PIERCE

          廠商性質(zhì)經(jīng)銷商

          所  在  地上海市

          更新時(shí)間:2024-05-31 10:05:29瀏覽次數(shù):1178次

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          品牌:PIERCE,貨號(hào):20665,產(chǎn)品名稱:DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP),1GM,規(guī)格: 1GM

          訂貨:,,

          DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)介紹

           

          Thermo Scientific BCA蛋白定量產(chǎn)品可以用于研究蛋白:蛋白間相互作用,衡量親和層析后的組分含量,估算從細(xì)胞裂解液里提取的膜蛋白含量和高通量篩選融合蛋白。

           

          DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)現(xiàn)貨供應(yīng)

           

          PIERCE

          20665

          DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP),1GM

          1 GM

          PIERCE

          20688

          Dimethyl Sulfoxide, Sequanal Grade (DMSO)

          950 ML

          PIERCE

          21004

          IgG Elution Buffer, 1 Liter

          1 LITER

           

          DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)品牌介紹

           

          美國(guó)Pierce公司(www.pierce.com)是蛋白質(zhì)化學(xué)、免疫學(xué)和層析等很多方面的和*。http://www.thermo.com.cn/Category828.html

           

          主要產(chǎn)品包括:

          1)化學(xué)發(fā)光體和比色底物

           

          2)分析試劑

           

          3)熒光標(biāo)記探針

           

          4)酶聯(lián)免疫分析試劑盒等

           

          DIMETHYL 3,3'-DITHIOBISPROPIONIMIDATE 2HCL(DTBP)

          僅用于科研

           

          Thermo Scientific Pierce DTBP is dimethyl 3,3'-dithiobispropionimidate, a crosslinker that contains amine-reactive imidoester groups around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

           

          Dimethyl dithiobispropionimidate (DTBP) is a homobifunctional, cleavable and membrane permeable cross-linker. It contains an amine-reactive imidoester at each end of an 8-atom spacer arm. Imidoesters react with primary amines at pH 8-10 to form stable amidine bonds. The amidine is protonated and therefore, like the lysine it modified, has a positive charge at physiological pH. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for imidoester crosslinking reagents.

           

          DTBP crosslinks can be easily reversed by the addition of 100-150 mM DTT at 37°C for 30 minutes. DMS, the non-cleavable analog of DTBP is available for applications that require a stable, non-cleavable bond. DTSSP, the NHS-ester analog of DTBP is also available for applications that require crosslinking at physiologic pH or greater stability of the crosslinked product at elevated pH. DTBP and DMS have essentially identical crosslinking activity toward primary amines.

           

          DTBP Highlights

          • Amine-reactive imidoester reacts rapidly with any primary amine-containing molecule to form amidine bonds
          • DTBP crosslinker is water soluble for compatibility with aqueous solutions
          • DTBP crosslinking can be easily reversed with DTT, TCEP or other reducing agents
          • High purity DTBP can be used to create high-purity conjugates
          • High purity DTBP can be used to create high-purity conjugates

          •  

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