目錄:北京索萊寶科技有限公司>>生化檢測試劑盒-常量法>>維生素C代謝系列>> BC0220抗壞血酸過氧化物酶(APX)測試盒 維生素C
CAS | 詳詢 | 純度 | 詳詢 |
---|---|---|---|
分子量 | 詳詢 | 分子式 | 詳詢 |
供貨周期 | 現(xiàn)貨 | 規(guī)格 | 50管/48樣 |
貨號 | BC0220 | 應(yīng)用領(lǐng)域 | 環(huán)保,化工,生物產(chǎn)業(yè),農(nóng)業(yè),綜合 |
主要用途 | APX(EC.1.11.1.11)是植物清除活性氧的重要抗氧化酶之一,也是抗壞血 |
試劑名稱 | 規(guī)格 | 保存條件 |
提取液 | 液體60 mL×1瓶 | 2-8℃保存 |
粉劑一 | 粉劑×1瓶 | 2-8℃保 |
試劑一 | 液體40 mL×1瓶 | 2-8℃保存 |
試劑二 | 粉劑×2瓶 | 2-8℃保存 |
試劑三 | 液體0.25mL×1支 | 2-8℃保存 |
提取液:臨用前將粉劑一倒入提取液中,溶液為懸濁液,搖勻使用即可,2-8℃可以保存12周。
試劑二:臨用前加入10 mL蒸餾水充分溶解;2-8℃可以保存1周。(由于試劑穩(wěn)定性較差,故多給一瓶)
試劑三:使用前先離心。臨用前根據(jù)樣本量取適量試劑三用蒸餾水稀釋100倍使用即可。
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取0.1g小薊樣本加入1mL提取液進(jìn)行勻漿研磨,取上清,之后按照測定步驟操作,用1mL石英比色皿測得計(jì)算ΔA空白管= A1空白管-A2空白管=0.668-0.659=0.009,ΔA測定管= A1測定管-A2測定管=0.958-0.824=0.134,按樣本質(zhì)量計(jì)算酶活得:
抗壞血酸過氧化物酶(APX)測試盒 維生素C 抗壞血酸過氧化物酶(APX)測試盒 維生素C
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