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Contrad 70保養(yǎng)Microcal itc200
點(diǎn)擊次數(shù):4238 發(fā)布時(shí)間:2013-5-29
Microcal itc200保養(yǎng),Microcal itc200維修與保養(yǎng),Microcal itc200如何保養(yǎng)
等溫滴定量熱儀(ITC)在單次實(shí)驗(yàn)中即可同時(shí)測(cè)定所有結(jié)合的熱力學(xué)參數(shù)(n,K,ΔH 和 ΔS)。可應(yīng)用于檢定蛋白、抗體、核酸、脂類(lèi)和其他生物分子間的相互作用;先導(dǎo)藥物的優(yōu)化;酶促反應(yīng)動(dòng)力學(xué)以及分子結(jié)構(gòu)修飾改變后的結(jié)合效果評(píng)估等。由于ITC 不僅僅可用于測(cè)定分子間的結(jié)合親和力,更能詳細(xì)的闡述分子間相互作用的機(jī)制,提供機(jī)構(gòu)分析必須的熱力學(xué)參數(shù),因此該方法正越來(lái)越多的被應(yīng)用于生物分子相互作用的研究。
• 不僅僅是親和力:一次試驗(yàn)即可同時(shí)確定所有的結(jié)合參數(shù),提供從其它結(jié)合試驗(yàn)無(wú)法獲取的所有信息
• 真實(shí)溶解中技術(shù):無(wú)需標(biāo)記或固定。 無(wú)緩沖液約束。 易于處理渾濁溶液
• 應(yīng)用普適性:高靈敏度地研究所有生物分子反應(yīng)情況
• 測(cè)量使用競(jìng)爭(zhēng)性結(jié)合技術(shù)的 nanomolar 與 picomolar 的結(jié)合常數(shù)
對(duì)Microcal itc200進(jìn)行定期保養(yǎng)可以提高實(shí)驗(yàn)質(zhì)量進(jìn)而獲得更
的實(shí)驗(yàn)結(jié)果。細(xì)胞的清潔度對(duì)數(shù)據(jù)質(zhì)量有明顯的影響。
After every run
• The system should be cleaned after each run using the Cell and Syringe Wash command.
Weekly
• Clean the cell(s)with 20% Contrad™ 70 (or 14% Decon™ 90).Use theDetergent Soak and Rinse (long) command and follow
the instructions to load the cellwith 20% Contrad 70 (or 14% Decon 90) and heatfor 30 minutes at 60°C. This should be done
whenever awaterrinse is insufficientto clean the cell properly. The easiestway to check ifthe cell needs cleaning is to check
thatthe baseline position is no more than 1 μcal/sec lowerthan the reference power setting in the ITCexperiment set up.
• Refillthe reference cell with de-ionized water.
• Visually inspectthe wash module while performing cell or syringe washing. Verify liquid is flowing to the waste bottle.
Monthly
• Inspectthe titration syringe carefully for breaks in the glass. Replace the syringe if it shows any sign of damage. Also inspect
the FPA tip(s) for wear.
• Clean the titration syringe afterreverse titrations (protein is loaded into the syringe) or monthly as preventive maintenance.
Pull 2 × 100 μl of 20% Contrad 70 (or 14% Decon 90) into the syringe and then rinse with water using Syringe rinse long on
the Accessory Station.
• Every 300 runs, orif it shows signs of wear,replace the plungertip