| 供貨周期 |
一周 |
規(guī)格 |
凍干粉 |
| 貨號 |
CMCC 51252 |
應用領域 |
醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥 |
| 主要用途 |
科學研究 |
CMCC 51252 痢疾志賀氏菌，現(xiàn)貨供應 ATCC|CMCC 菌種 上海復祥生物公司現(xiàn)貨供應美國典型培養(yǎng)物保藏中心（ATCC）菌種。,*,所謂“踏破鐵鞋無覓處.得來全不費工夫!
CMCC 51252 痢疾志賀氏菌
感謝您使用本單位的微生物菌種，我們有可用于科研、教學、生產(chǎn)的菌種，基本覆蓋了環(huán)境、工業(yè)、農(nóng)業(yè)、分析、食、藥用真菌行業(yè)各類生產(chǎn)和科研用微生物，其中許多優(yōu)良菌株的生產(chǎn)性能具有較高水平。
凍干菌種的復蘇培養(yǎng)：
1凍干管開封：用浸過75%酒精的脫脂棉擦凈凍干管，用火焰加熱其頂端，滴少量無菌水至加熱頂端使之破裂，用銼刀或鑷子敲下已破裂的凍干管頂端。
2菌種恢復培養(yǎng)：用無菌吸管 0.3-0.5毫升適宜到液體培養(yǎng)基，滴入凍干管內(nèi)輕輕振蕩，使凍干菌體溶解呈懸浮狀，吸取全部菌體懸浮液，移植于1-2支建議的斜面培養(yǎng)基上，并在建議的溫度下培養(yǎng)。
CMCC 51252 痢疾志賀氏菌注意事項
1、微生物菌種應保藏于低溫、清潔和干燥的地方，室溫放置時間過長會導致菌種衰退；
2、菌種操作應在無菌條件下進行，防止雜菌污染；
3、斜面菌種保藏時間通常為3-6個月，應根據(jù)菌種狀況及時轉(zhuǎn)接；凍干菌種保藏時間通常為2-15年；
| Organism: | Escherichia coli (Migula) Caslani and Chalmers |
| Designations: | EDL 931 |
| Isolation: | feces, human |
| Depositor: | GK Morris |
| History: | ATCC <<--GK Morris<<--J.G. Wells |
| Biosafety Level: | 2 |
| Shipped: | freeze-dried |
| Growth Conditions: | ATCC medium18: Trypticase soy agar
Temperature: 37.0°C |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
| Antigenic Properties: | serotype O157:H7 |
| Cross References: | Nucleotide (GenBank) : AF305917Escherichia coli O157:H7 nonfunctional beta-glucuronidase (uidA) gene, complete cds. |
| Comments: | Hemorrhagic colitis [9698] Cytotoxic activity [8277] |
| References: | 8277: Beery JT, et al. Cytotoxic activity of Escherichia coli O157:H7 culture filtrate on the mouse colon and kidney. Curr. Microbiol. 11: 335-342, 1984.
9698: Riley LW, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681-685, 1983. PubMed: 6338386 |
14
