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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:上海復(fù)祥生物科技有限公司>>細(xì)胞庫(kù)>>小鼠正常細(xì)胞>>CRL-12103FDC-P1 小鼠正常骨髓細(xì)胞

          FDC-P1 小鼠正常骨髓細(xì)胞

          參   考   價(jià):面議
          具體成交價(jià)以合同協(xié)議為準(zhǔn)

          產(chǎn)品型號(hào):CRL-12103

          品       牌:ATCC

          廠商性質(zhì):生產(chǎn)商

          所  在  地:上海市

          更新時(shí)間:2024-11-14 20:47:05瀏覽次數(shù):2104

          聯(lián)系我時(shí),請(qǐng)告知來(lái)自 化工儀器網(wǎng)
          供貨周期 一周 規(guī)格 T25
          貨號(hào) CRL-12103 應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,化工,生物產(chǎn)業(yè),制藥
          主要用途 科學(xué)研究
          CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

          CRL-12103 FDC-P1 小鼠正常骨髓細(xì)胞的詳細(xì)介紹

          CRL-12103 FDC-P1 小

          鼠正常骨髓細(xì)胞

          ATCC® Number:CRL-12103™  Price:$355.00
          Designations:FDC-P1

          Depositors:University of Virginia Patent Foundation

          Biosafety Level:1

          Shipped:frozen

          Medium & Serum:See Propagation

          G
          th Properties:suspension
          Organism:Mus musculus (mouse)
          Morphology:lymphoblas
          row




          t


          Source:Organ: bone marrow
          Strain: DBA/2
          Disease: normal


          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
          CRL-12103

          Applications:transfection host (Nucleofection technology from Lonza)

          Antigen Expression:Thy-1.2 (24%)

          Cytogenetic Analysis:diploid

          Comments:The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).

          They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth.



          Propagation:ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040).
          Temperature: 37.0°C


          Subculturing:Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
          Medium Renewal: Every 2 to 3 days


          Preservation:Freeze medium: Complete growth medium, 90%; DMSO, 10%
          Storage temperature: liquid nitrogen vapor temperature


















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