低價供應(yīng) CRL-1469 PANC-1人胰腺癌細(xì)胞 CRL-1469

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CRL-1469 PANC-1人胰腺癌細(xì)胞
ATCC? Number:  CRL-1469?      
Designations:  PANC-1  
Depositors:   M Lieber  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: pancreas 
Tissue: duct 
Disease: epithelioid carcinoma 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 CRL-1469 PANC-1人胰腺癌細(xì)胞
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE? Transfection Reagents) 
DNA Profile (STR): Amelogenin: X 
CSF1PO: 10,12 
D13S317: 11 
D16S539: 11 
D5S818: 11,13 
D7S820: 8,10 
THO1: 7,8 
TPOX: 8,11 
vWA: 15 
Cytogenetic Analysis: Chromosome studies indicate a modal number of 63 with 3 distinct marker chromosomes and a small ring chromosome. This is a hypertriploid human cell line. The modal chromosome number was 61, occurring in 32% of cells., However, cells with 63 chromosomes also occurred at a high frequency (22%). The rate of cells with higher ploidies was 8.5%. 
Isoenzymes:  G6PD, B 
Age:  56 years  
Gender:  male  
Ethnicity:  Caucasian  
Comments: Growth is inhibited by 1 unit/ml L-asparaginase. 
The cells will grow in soft agar. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, and rinse with 0.25% trypsin, 0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks. 
Preservation:  culture medium 95%; DMSO, 5% 
Doubling Time:  52 hrs  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020 
References: 22850: Lieber M, et al. Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas. Int. J. Cancer 15: 741-747, 1975. PubMed: 1140870
22859: Wu MC, et al. Mechanism of sensitivity of cultured pancreatic carcinoma to asparaginase. Int. J. Cancer 22: 728-733, 1978. PubMed: 363626
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903 
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COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細(xì)胞
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HCT-8    人回盲腸癌細(xì)胞
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Hepa1-6    小鼠肝癌細(xì)胞
HepG2    人肝癌細(xì)胞
hFOB1.19    人永生化成骨細(xì)胞
HGC-27    人胃癌細(xì)胞
Hs 746T    人胃癌細(xì)胞
HT-29    人結(jié)腸癌細(xì)胞
KB    人口腔表皮樣癌細(xì)胞
LoVo    人結(jié)腸癌細(xì)胞
LLC-PK1    豬腎近曲小管上皮細(xì)胞
MCF-10A    人正常乳腺上皮細(xì)胞
MCF-7    人乳腺癌細(xì)胞
MDA-MB-231    人乳腺癌細(xì)胞
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NCI-H460    人大細(xì)胞肺癌細(xì)胞
NIH-3T3    小鼠成纖維細(xì)胞
NUGC-3    人胃癌細(xì)胞
Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞
SGC-7901    人胃癌細(xì)胞
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U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤
BT-474    人乳腺導(dǎo)管癌細(xì)胞
PANC-1    人胰腺癌細(xì)胞
HEK293    人胚腎細(xì)胞
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HL-7702    人正常肝細(xì)胞
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RWPE-1    人正常前列腺上皮細(xì)胞
CAL 27    人舌鱗癌細(xì)胞
Calu-3    人肺腺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
DLD-1    人結(jié)直腸腺癌上皮細(xì)胞
5637.00     人膀胱癌細(xì)胞
SK-OV-3    人卵巢癌細(xì)胞
GBC-SD    人膽囊癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
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MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購買
PC9    人肺癌細(xì)胞
PC14    人肺癌細(xì)胞
BxPC    人原位胰腺腺癌細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MDCK    狗腎上皮細(xì)胞
3D4/21    豬肺泡巨噬細(xì)胞
    
    
    
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A498    人腎癌細(xì)胞
143B    
WEHI-231    小鼠B淋巴細(xì)胞
BN    
P9R    小鼠骨髓基質(zhì)細(xì)胞
P9    小鼠骨髓基質(zhì)細(xì)胞
F98    大鼠腦膠質(zhì)瘤細(xì)胞
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NCIH1650-M3    人非小細(xì)胞肺癌細(xì)胞
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EC9706    人食管癌細(xì)胞
CNE    人鼻咽癌細(xì)胞
H322    人非小細(xì)胞肺癌細(xì)胞
Panc O2    
S8    BEL7402 人肝癌細(xì)胞系
R5    細(xì)胞系購買
Pig MSC    豬骨髓間質(zhì)干細(xì)胞
HCAEC    人冠狀動脈內(nèi)皮細(xì)胞
marc-145    猴胚胎腎上皮細(xì)胞
3D4/21    豬肺泡巨噬細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MADB-106    大鼠乳腺癌細(xì)胞
Sf9    昆蟲卵巢細(xì)胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞
PC14    人肺癌細(xì)胞
PC9    人肺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
CAL 27    人舌鱗癌細(xì)胞
HOC    肝卵圓細(xì)胞
EJ    人膀胱癌細(xì)胞

 CRL-1469 PANC-1人胰腺癌細(xì)胞
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Raw-Blue    小鼠單核細(xì)胞白血病細(xì)胞
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HCT-8    人回盲腸癌細(xì)胞
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 CRL-1469 PANC-1人胰腺癌細(xì)胞