低價供應(yīng) HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞

HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞 的詳細(xì)介紹

ATCC? Number:  HTB-177?        
Designations:  NCI-H460 [H460]  
Depositors:   AF Gazdar, JD Minna  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: lung 
Disease: carcinoma; large cell lung cancer 
Derived from metastatic site: pleural effusion 
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 HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞
Isolation:  Isolation date: 1982 
Tumorigenic: Yes  
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 13 
D16S539: 9 
D5S818: 9,10 
D7S820: 9,12 
THO1: 9.3 
TPOX: 8 
vWA: 17 
Cytogenetic Analysis: modal numbr = 57; range = 53 to 65.This is a hypotriploid human cell line. The modal chromosome number is 57 although cells with 58 chromosomes occurred with a comparable frequency. The frequency of higher ploidies was 1.7%. Seven marker chromosomes, der(9)t(1;9)(q21;p24), der(9)t(7;9)(p11;p22), t(10q14q), der(16)t(7;16)(q11.23;q22), a small ring (about 1/2 the size of a G chromosome) and two others, were common to all cells. Three other markers were found in some cells only. The markers, t(7;9) and t(7;16) were mostly paired. Normal N9 was absent, and N7 and N16 had only a single copy per cell. Two copies each of the X and the Y were present in all cells. 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1-2 
Me-2, 1 
PGM1, 1 
PGM3, 1 
Gender:  male  
Comments: The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. 
The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. 
The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C   HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended 
Medium Renewal: Twice per week 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Doubling Time:  23 hrs in medium with serum; 42 to 60 hrs in serum  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020 
References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876
1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494
22434: Brower M, et al. Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644   HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞
32488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241 
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CaCo-2    人結(jié)腸癌細(xì)胞
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Hepa1-6    小鼠肝癌細(xì)胞
HepG2    人肝癌細(xì)胞
hFOB1.19    人永生化成骨細(xì)胞
HGC-27    人胃癌細(xì)胞
Hs 746T    人胃癌細(xì)胞
HT-29    人結(jié)腸癌細(xì)胞
KB    人口腔表皮樣癌細(xì)胞
LoVo    人結(jié)腸癌細(xì)胞
LLC-PK1    豬腎近曲小管上皮細(xì)胞
MCF-10A    人正常乳腺上皮細(xì)胞
MCF-7    人乳腺癌細(xì)胞
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MGC80-3    人胃癌細(xì)胞
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NCI-H460    人大細(xì)胞肺癌細(xì)胞
NIH-3T3    小鼠成纖維細(xì)胞
NUGC-3    人胃癌細(xì)胞
Raw264-7    小鼠單核細(xì)胞白血病細(xì)胞
SGC-7901    人胃癌細(xì)胞
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U-373MG    人腦神經(jīng)膠質(zhì)瘤細(xì)胞
U-87 MG    人腦星形膠質(zhì)母細(xì)胞瘤
BT-474    人乳腺導(dǎo)管癌細(xì)胞
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 HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞
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CAL 27    人舌鱗癌細(xì)胞
Calu-3    人肺腺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
DLD-1    人結(jié)直腸腺癌上皮細(xì)胞
5637.00     人膀胱癌細(xì)胞
SK-OV-3    人卵巢癌細(xì)胞
GBC-SD    人膽囊癌細(xì)胞
BEL7402 人肝癌細(xì)胞系
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MRC-5    人胚肺成纖維細(xì)胞     細(xì)胞系購買
PC9    人肺癌細(xì)胞
PC14    人肺癌細(xì)胞
BxPC    人原位胰腺腺癌細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MDCK    狗腎上皮細(xì)胞
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Panc O2    低價供應(yīng) HTB-177 NCI-H460 人大細(xì)胞肺癌細(xì)胞
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R5    細(xì)胞系購買
Pig MSC    豬骨髓間質(zhì)干細(xì)胞
HCAEC    人冠狀動脈內(nèi)皮細(xì)胞
marc-145    猴胚胎腎上皮細(xì)胞
3D4/21    豬肺泡巨噬細(xì)胞
PK（15）    豬腎上皮細(xì)胞
MADB-106    大鼠乳腺癌細(xì)胞
Sf9    昆蟲卵巢細(xì)胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細(xì)胞
PC14    人肺癌細(xì)胞
PC9    人肺癌細(xì)胞
Eca-109    人食管癌細(xì)胞
CAL 27    人舌鱗癌細(xì)胞
HOC    肝卵圓細(xì)胞
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SNU-638    人胃癌細(xì)胞
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