HTB-38 HT-29 人結(jié)腸癌細胞 

低價供應(yīng) HTB-38 HT-29 人結(jié)腸癌細胞
ATCC? Number:  HTB-38?       
Designations:  HT-29  
Depositors:   J Fogh  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

 
Source: Organ: colon 
Disease: colorectal adenocarcinoma 
Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.  
Isolation:  Isolation date: 1964  低價供應(yīng)
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE? Transfection Reagents) 
Receptors: human adrenergic alpha2A [23560] 
urokinase receptor (u-PAR) 
vitamin D (moderate expression) 
urokinase receptor (u-PAR); vitamin D (moderate expression) 
Virus Susceptibility: human immunodeficiency virus (HIV, LAV) 
Tumorigenic: Yes  
Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -  
Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5 
DNA Profile (STR): Amelogenin: X 
CSF1PO: 11,12 
D13S317: 11,12 
D16S539: 11,12 
D5S818: 11,12 
D7S820: 10 
THO1: 6,9 
TPOX: 8,9 
vWA: 17,19 
Cytogenetic Analysis: modal number = 71; range = 68 to 72.
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis. 
Isoenzymes:  AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1-2 
Me-2, 1   
PGM1, 1-2 
PGM3, 1-2 
Age:  44 years adult  
Gender:  female  
Ethnicity:  Caucasian  
Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected. 
There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.

Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:8 is recommended 
Medium Renewal: 2 to 3 times per week 
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor temperature 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
derivative:ATCC CCL-218
purified DNA:ATCC HTB-38D 

MLE-12 小鼠肺上皮細胞   
saos-2 人成骨肉瘤細胞
MA104 非洲綠猴胚胎腎上皮細胞
ball-1 細胞
nci-h226 人肺鱗癌細胞
MCA38 小鼠結(jié)腸癌細胞
LTPA 小鼠胰腺癌細胞 
NCI-H23 人非小細胞肺癌細胞
293.00     人胚腎細胞

低價供應(yīng)
293FT    人胚腎細胞-用于慢病毒包裝
293TN    人胚腎細胞--用于慢病毒包裝
293AV    人胚腎細胞-用于腺病毒包裝
293T    人胚腎細胞
208F    大鼠成纖維細胞
786-O    人腎透明細胞腺癌細胞
A172    人腦膠質(zhì)母細胞瘤細胞
A549    人肺癌細胞 細胞系購買
AGS    人胃癌細胞
BEL7402 人肝癌細胞系
AZ-521    人胃癌細胞
B16F0    小鼠黑色素瘤細胞
Bel-7402    人肝癌細胞
Bel-7404    人肝癌細胞
BGC-823    人胃癌細胞
C6    大鼠腦膠質(zhì)瘤細胞
SW480    人結(jié)腸腺癌細胞
CaCo-2    人結(jié)腸癌細胞
Cho-K1    倉鼠卵巢細胞亞株
COS-7    非洲綠猴SV40轉(zhuǎn)化的腎細胞
GES-1    人胃上皮細胞
HCT-8    人回盲腸癌細胞
HeLa    人宮頸癌細胞
Hepa1-6    小鼠肝癌細胞
HepG2    人肝癌細胞
hFOB1.19    人永生化成骨細胞
HGC-27    人胃癌細胞
Hs 746T    人胃癌細胞
HT-29    人結(jié)腸癌細胞
KB    人口腔表皮樣癌細胞
LoVo    人結(jié)腸癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
MCF-10A    人正常乳腺上皮細胞
MCF-7    人乳腺癌細胞
MDA-MB-231    人乳腺癌細胞
MGC80-3    人胃癌細胞
MKN-45    人胃癌細胞

  
NCI-H460    人大細胞肺癌細胞
NIH-3T3    小鼠成纖維細胞
NUGC-3    人胃癌細胞
Raw264-7    小鼠單核細胞白血病細胞
SGC-7901    人胃癌細胞
SMMC-7721    人肝癌細胞
SNU-638    人胃癌細胞
SNU-739    人肝癌細胞
Tca8113    人舌鱗癌細胞    細胞系購買
U251    人腦膠質(zhì)瘤細胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞
U-87 MG    人腦星形膠質(zhì)母細胞瘤
BT-474    人乳腺導(dǎo)管癌細胞
PANC-1    人胰腺癌細胞
HEK293    人胚腎細胞
Hep3B    人肝癌細胞
HL-7702    人正常肝細胞
Raw-Blue    小鼠單核細胞白血病細胞
WEHI-231    小鼠B淋巴細胞
OPM-2    人骨髓瘤細胞
NCI-H929    人骨髓瘤細胞

低價供應(yīng)
U266    人骨髓瘤細胞
EJ    人膀胱癌細胞
HOC    大鼠肝卵圓細胞
RWPE-1    人正常前列腺上皮細胞
CAL 27    人舌鱗癌細胞
Calu-3    人肺腺癌細胞
Eca-109    人食管癌細胞
DLD-1    人結(jié)直腸腺癌上皮細胞
5637.00     人膀胱癌細胞
SK-OV-3    人卵巢癌細胞
GBC-SD    人膽囊癌細胞
BEL7402 人肝癌細胞系
SW620    人結(jié)腸癌細胞
MRC-5    人胚肺成纖維細胞     細胞系購買
PC9    人肺癌細胞
PC14    人肺癌細胞
BxPC    人原位胰腺腺癌細胞
PK（15）    豬腎上皮細胞
MDCK    狗腎上皮細胞
3D4/21    豬肺泡巨噬細胞
    
    
    
MHCC-97L    人肝癌細胞（低轉(zhuǎn)移）
MHCC-97H    人肝癌細胞系（高轉(zhuǎn)移）
MiaCaPa-2    人胰腺癌細胞
HN13    口腔鱗狀細胞癌細胞
Bend3    小鼠腦微血管內(nèi)皮細胞
QBC939    人膽管癌細胞
Ishikawa    人子宮內(nèi)膜癌細胞
A498    人腎癌細胞
143B    
WEHI-231    小鼠B淋巴細胞
BN    
P9R    小鼠骨髓基質(zhì)細胞
P9    小鼠骨髓基質(zhì)細胞
F98    大鼠腦膠質(zhì)瘤細胞
NCIH1299    人非小細胞肺癌細胞
NCIH1650-M3    人非小細胞肺癌細胞
293LVT    人胚腎細胞--用于慢病毒包裝
EC9706    人食管癌細胞
CNE    人鼻咽癌細胞
H322    人非小細胞肺癌細胞
Panc O2    
S8    BEL7402 人肝癌細胞系
R5    細胞系購買
Pig MSC    豬骨髓間質(zhì)干細胞
HCAEC    人冠狀動脈內(nèi)皮細胞
marc-145    猴胚胎腎上皮細胞
3D4/21    豬肺泡巨噬細胞
PK（15）    豬腎上皮細胞
MADB-106    大鼠乳腺癌細胞
Sf9    昆蟲卵巢細胞
DADY    
HUVEC[HUV-EC-C]    人臍靜脈內(nèi)皮細胞
PC14    人肺癌細胞

低價供應(yīng)
PC9    人肺癌細胞
Eca-109    人食管癌細胞
CAL 27    人舌鱗癌細胞
HOC    肝卵圓細胞
EJ    人膀胱癌細胞

HGC-27 人胃癌細胞（未分化
U266    人骨髓瘤細胞
293-mTLR5    人胚腎細胞
Raw-Blue    小鼠單核細胞白血病細胞
HL-7702    人正常肝細胞
Hep3B    人肝癌細胞
U-373MG    人腦神經(jīng)膠質(zhì)瘤細胞
Tca-8113    人舌鱗癌細胞
SNU-739    人肝癌細胞
SNU-638    人胃癌細胞
NUGC-3    人胃癌細胞
NIH-3T3    小鼠成纖維細胞
MKN-45    人胃癌細胞
LLC-PK1    豬腎近曲小管上皮細胞
Hs 746T    人胃癌細胞
Hepa1-6    小鼠肝癌細胞
HCT-8    人回盲腸癌細胞
BEL7402 人肝癌細胞系
HaCaT    人永生化表皮細胞
GES-1    人胃上皮細胞
B16-F0    小鼠黑色素瘤細胞
AZ-521    人胃癌細胞
786-O    人腎透明細胞腺癌細胞
208F    大鼠成纖維細胞
293TN    人胚腎細胞--用于慢病毒包裝
293FT    人胚腎細胞-用于慢病毒包裝
GP-293    人胚腎細胞
293-L.P.    人胚腎細胞