CCL-244 HCT-8 人回盲腸癌細(xì)胞 ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|菌種原代細(xì)胞、細(xì)胞系。細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！
CCL-244 HCT-8 人回盲腸癌細(xì)胞 的詳細(xì)介紹
CCL-244 HCT-8 人回盲腸癌細(xì)胞
ATCC? Number: CCL-244?
Designations: HCT-8 [HRT-18]
Depositors: A Sobrero
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: epithelial
CCL-244
Source: Organ: colon
Disease: ileocecal colorectal adenocarcinoma
Cellular Products: carcinoembryonic antigen (CEA) 0.5 ng/10 exp6 cells/10 days; alkaline phosphatase; keratin
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Tumorigenic: Yes
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 8,11
D16S539: 12,13
D5S818: 13
D7S820: 10,12,11.3
THO1: 7,9.3
TPOX: 8,11
vWA: 18,19
Isoenzymes: AK-1, 1
ES-D, 1-2 CCL-244
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 67 years *
Gender: male
Comments: The HCT-8 line is identical to the HRT-18 cell line.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: RPMI 1640 medium with sodium pyruvate (1 mM); 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, rinse cell sheet twice with 0.25% trypsin, 0.02% EDTA in Hanks' BSS and incubate at 37C with the trypsin, EDTA for 10 to 15 minutes. Remove trypsin, EDTA, disperse cells in fresh medium, centrifuge, resuspend cells in medium and dispense into new flasks.
Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
References: 1052: Tompkins WA, et al. Cultural and antigenic properties of newly established cell strains derived from adenocarcinomas of the human colon and rectum. J. Natl. Cancer Inst. 52: 1101-1110, 1974. PubMed: 4826581
26064: Nelson-Rees WA, et al. Distinctive banded marker chromosomes of human tumor cell lines. Int. J. Cancer 16: 74-82, 1975. PubMed: 1058173
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293 CCL-244
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