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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機(jī)> CCL-25-WISH 人羊膜細(xì)胞

          [供應(yīng)]CCL-25-WISH 人羊膜細(xì)胞

          貨物所在地:上海上海市

          產(chǎn)地:美國(guó)

          更新時(shí)間:2024-04-01 16:25:27

          有效期:2024年4月1日 -- 2025年4月1日

          已獲點(diǎn)擊:100

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          CCL-25 WISH 人羊膜細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

          CCL-25 WISH 人羊膜細(xì)胞

          ATCC® Number:CCL-25™    Price:$323.00
          Additional information about this cell line
          Designations:WISH
          Depositors: L Hayflick
          Biosafety Level:2
          Shipped:frozen
          Medium & Serum:See Propagation
          Growth Properties:adherent
          Organism:Homo sapiens (human)
          Morphology:epithelial
           
          Source:Organ: HeLa contaminant
          Cellular Products:keratin
          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)
          Reverse Transcript:negative
          DNA Profile (STR):Amelogenin: X 
          CSF1PO: 9,10 
          D13S317: 13.3 
          D16S539: 9,10 
          D5S818: 11,12 
          D7S820: 8,12 
          THO1: 7 
          TPOX: 8,12 
          vWA: 16,18
          Isoenzymes:G6PD, A
          HeLa Markers:Y
          Comments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. 
          The cells are positive for keratin by immunoperoxidase staining. 
          NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
          Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
          Temperature: 37.0°C
          Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:10 is recommended 
          Medium Renewal: Twice per week 
          Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
          Preservation:culture medium 95%; DMSO, 5%
          Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
          recommended serum:ATCC 30-2020
          References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous c*tion. Exp. Cell Res. 23: 14-20, 1961. PubMed:13712490
          26034: . . Am. J. Dis. Child. 103: 460, 1962.

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