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        1. 上海復祥生物科技有限公司

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          [供應(yīng)]CCL-13-Chang liver 人張氏肝細胞

          貨物所在地:上海上海市

          產(chǎn)地:美國

          更新時間:2024-04-01 16:25:17

          有效期:2024年4月1日 -- 2025年4月1日

          已獲點擊:143

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          CCL-13 Chang liver 人張氏肝細胞,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

          CCL-13 Chang liver 人張氏肝細胞

          ATCC® Number:CCL-13™    Price:$323.00
          Additional information about this cell line
          Designations:HeLa [Chang Liver]
          Depositors: RS Chang
          Biosafety Level:2 [Cells contain human papilloma virus (HPV-18) ]
          Shipped:frozen
          Medium & Serum:See Propagation
          Growth Properties:adherent
          Organism:Homo sapiens (human)
          Morphology:epithelial
           
          Source:Organ: HeLa contaminant
          Cellular Products:keratin
          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Applications:transfection host (Roche FuGENE® Transfection Reagents)
          Virus Susceptibility:poliovirus 1, 2, 3; adenovirus 3; vesicular stomatitis (Indiana)
          Tumorigenic:Yes
          Reverse Transcript:negative
          DNA Profile (STR):Amelogenin: X 
          CSF1PO: 10 
          D13S317: 12,13.3 
          D16S539: 9,10 
          D5S818: 12 
          D7S820: 8,12 
          THO1: 7 
          TPOX: 8,12 
          vWA: 16,18
          Isoenzymes:G6PD, A
          HeLa Markers:Y
          Comments:This line was originally thought to be derived from normal liver tissue, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. 
          The cells are positive for keratin by immunoperoxidase staining. 
          NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
          Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
          Temperature: 37.0°C
          Subculturing:Protocol: Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution, rinse and remove. Allow flask to sit at room temperature (or at 37C) until cells detach (approximay 10 minutes). Add fresh medium, aspirate and dispense into new flasks. 
          Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended 
          Medium Renewal: 2 to 3 times per week
          Preservation:Complete growth medium, 95%; DMSO, 5%
          References:22213: Chang RS. Continuous subc*tion of epithelial-like cells from normal human tissues. Proc. Soc. Exp. Biol. Med. 87: 440-443, 1954. PubMed: 13237268
          26122: Murphy WH, Landau BJ. Clonal variation and interaction of cells with viruses. Natl. Cancer Inst. Monogr. 7: 249-271, 1962. PubMed:14477443
          32352: Benn J, et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004

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