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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機(jī)> CRL-9520-SV-HUC-1 人膀胱上皮永生化細(xì)胞

          [供應(yīng)]CRL-9520-SV-HUC-1 人膀胱上皮永生化細(xì)胞

          貨物所在地:上海上海市

          產(chǎn)地:美國(guó)

          更新時(shí)間:2024-04-18 10:31:55

          有效期:2024年4月18日 -- 2025年4月18日

          已獲點(diǎn)擊:330

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          CRL-9520 SV-HUC-1 人膀胱上皮永生化細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

           

          CRL-9520 SV-HUC-1 人膀胱上皮永生化細(xì)胞

          ATCC® Number:CRL-9520™    Price:$417.00
          Designations:SV-HUC-1
          Depositors: Wisconsin Alumni Res. Fndn.
          Biosafety Level:2 [CELLS CONTAIN ADENOVIRUS ]
          Shipped:frozen
          Medium & Serum:See Propagation
          Growth Properties:adherent
          Organism:Homo sapiens (human)
          Morphology:epithelial
           
          Source:Organ: ureter 
          Tissue: uroepithelium 
          Cell Type: epithelial SV40 immortalizedSV40 transformed
          Cellular Products:uroepithelial keratins; SV40 T antigen
          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Tumorigenic:No
          Cytogenetic Analysis:The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88). Both populations consistently showed seven marker chromosomes: 5p+, del(6)(p11), 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
          Age:11 years adult
          Gender:male
          Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
          Temperature: 37.0°C
          Subculturing:Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. Add fresh medium, aspirate and dispense into new flasks. 
          Subc*tion Ratio: 1:2 to 1:5 
          Medium Renewal: Every 2 to 3 days
          Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% 
          Storage temperature: liquid nitrogen vapor phase
          Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
          recommended serum:ATCC 30-2020
          derivative:ATCC CRL-9519
          References:3992: Reznikoff CA, Christian BJ. Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990

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