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        1. 上海復(fù)祥生物科技有限公司

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          [供應(yīng)]3T3-L1 小鼠脂肪細(xì)胞

          貨物所在地:上海上海市

          更新時(shí)間:2024-07-11 21:00:05

          有效期:2024年7月11日 -- 2025年1月11日

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          3T3-L1 小鼠脂肪細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞。細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!
          3T3-L1 小鼠脂肪細(xì)胞 的詳細(xì)介紹
          3T3-L1 小鼠脂肪細(xì)胞
          ATCC® Number: CL-173™    Price:
          Designations: 3T3-L1
          Depositors:  Massachusetts Institute of Technology
          Biosafety Level: 1
          Shipped: frozen
          Medium & Serum: See Propagation
          Growth Properties: adherent
          Organism: Mus musculus (mouse)
          Morphology: fibroblast
          3T3-L1 小鼠脂肪細(xì)胞
          Source: Organ: embryo
          Cell Type: fibroblast
          Cellular Products: triglycerides [3491]
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Applications: transfection host (Nucleofection technology from Lonza
          Roche FuGENE® Transfection Reagents
          Receptors: insulin, expressed
          Reverse Transcript: negative
          Age: embryo
          Comments: L1 is a continuous substrain of 3T3 (Swiss albino) developed through clonal isolation. The cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation [PubMed ID: 4426090].
          Tested and found negative for ectromelia virus (mousepox).
          This line is also designated as ATCC CCL-92.1.ATCC CL-173 was deposited in 1974 without passage number information from the depositor. At the time of submission, ATCC prepared approximay 30 vials of seed stock at about 4 passages beyond the original depositor material (passage number: unknown +4).
          Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
          Atmosphere: air, 95%; carbon dioxide (CO2), 5%
          Temperature: 37.0°C
          Growth Conditions: The serum used is important in culturing this line. Calf serum is recommended and not fetal bovine serum.
          Subculturing: Protocol: Never allow culture to become compley confluent.
          1. Remove and discard culture medium.
          2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
          3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
          4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
          5. Add appropriate aliquots of the cell suspension to new culture vessels.
            The recommended inoculum is 2 to 3 X 10(3) cells/sq. cm. Subculture before cultures become 70 to 80% confluent or when cells reach 5 to 6 X10(4) viable cells/sq. cm.
          6. Incubate cultures at 37C.

          Interval: Every three days
          Medium Renewal: 2 to 3 times per week
          Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
          Storage temperature: liquid nitrogen vapor phase
          Doubling Time: 14 hrs
          Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
          formerly distributed as:ATCC CCL-92.1
          0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
          Cell culture tested DMSO:ATCC 4-X
          Recommended serum: ATCC 30-2030
          References: 886: Green H, Meuth M. An established pre-adipose cell line and its differentiation in culture. Cell 3: 127-133, 1974. PubMed: 4426090
          3491: Green H. Triglyceride-accumulating clonal cell line. US Patent 4,003,789 dated Jan 18 1977
          32373: Goodrum FD, et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260

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