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Receptors:
complement （C3）
Comments:
The growth of WEHI-3 is inhibited by 4 ng/ml LPS and blocked by higher concentrations. Dextran sulfate at 30 to 40 mcg/ml also inhibits growth. Latex beads are phagocytized but are not toxic. Zymosan and BCG are phagocytized and block growth. The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity. Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10% Temperature: 37.0°C
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml. Adherent cells may be harvested by scraping.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2005 recommended serum:ATCC 30-2020
References:
1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031 1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PubMed: 318922 23431: McKean DJ, et al. Major histocompatibility complex-restricted antigen presentation to antigen-reactive T cells by B lymphocyte tumor cells. J. Exp. Med. 154: 1419-1431, 1981. PubMed: 6170720 33035: Boring L, et al. Molecular cloning and functional expression of murine JE （monocyte chemoattractant protein 1） and murine macrophage inflammatory protein 1alpha receptors. J. Biol. Chem. 271: 7551-7558, 1996. PubMed: 8631787
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