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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機(jī)> MC 3T3-E1 小鼠成骨細(xì)胞

          [供應(yīng)]MC 3T3-E1 小鼠成骨細(xì)胞

          貨物所在地:上海上海市

          更新時(shí)間:2024-07-11 21:00:05

          有效期:2024年7月11日 -- 2025年1月11日

          已獲點(diǎn)擊:225

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          MC 3T3-E1 小鼠成骨細(xì)胞 ,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!
          MC 3T3-E1 小鼠成骨細(xì)胞 的詳細(xì)介紹
          MC 3T3-E1  小鼠成骨細(xì)胞
          ATCC® Number: CRL-2593™    Price: $324.00
          Designations: MC3T3-E1 Subclone 4
          Depositors:  RT Franceschi
          Biosafety Level: 1
          Shipped: frozen
          Medium & Serum: See Propagation
          Growth Properties: adherent
          Organism: Mus musculus (mouse)
          Morphology: fibroblast

          Source: Organ: bone
          Strain: C57BL/6
          Tissue: calvaria
          Cell Type: preosteoblast;
          Cellular Products: collagen [51540]
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Tumorigenic: Yes
          Age: newborn newborn
          Comments: A series of subclones were isolated from the cloned but phenotypically heterogeneous MC3T3-E1 cell line. The subclones were selected for high or low osteoblast differentiation and mineralization after growth in medium containing ascorbic acid. The MC3T3-E1 Subclone 4 (ATCC CRL-2593) and the MC3T3 Subclone 14 (ATCC CRL-2594) lines exhibit high levels of osteoblast differentiation after growth in ascorbic acid and 3 to 4 mM inorganic phosphate. They form a well mineralized extracellular matrix (ECM) after 10 days [PubMed ID: 10352097].
          The MC3T3 Subclone 24 (ATCC CRL-2595) and the MC3T3 Subclone 30 (ATCC CRL-2596) lines exhibit poor osteoblast differentiation after growth in ascorbic acid. They do not form ECM. They can be used as negative controls for Subclones 4 and 14 [PubMed ID: 10352097].
          Mineralizing subclones selectively express mRNAs for the osteoblast markers, bone sialoprotein (BSP), osteocalcin (OCN), and the parathyroid hormone (PTH)/parathyroid hormone-related protein (PTHrP) receptor.S ubclones with both high and low differentiation potential produce similar amounts of collagen in culture and express comparable basal levels of mRNA encoding Osf2/Cbfa1, an osteoblast-related transcription factor [PubMed ID: 10352097].

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