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        1. 上海復祥生物科技有限公司

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          [供應]CTLL-2 小鼠T細胞 IL-2依賴型

          貨物所在地:上海上海市

          更新時間:2024-07-11 21:00:05

          有效期:2024年7月11日 -- 2025年1月11日

          已獲點擊:348

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          CTLL-2 小鼠T細胞IL-2依賴型,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!

          CTLL-2 小鼠T細胞IL-2依賴型,原代細胞|細胞系|細胞株|菌種;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件!


           

          CTLL-2 小鼠T細胞 IL-2依賴型 的詳細介紹
          CTLL-2  小鼠T細胞IL-2依賴型
          ATCC® Number: TIB-214™    Price: $289.00
          Designations: CTLL-2
          Depositors:  S Gillis
          Biosafety Level: 1
          Shipped: frozen
          Medium & Serum: See Propagation
          Growth Properties: suspension
          Organism: Mus musculus (mouse)
          Morphology: lymphoblast
          CTLL-2  小鼠T細胞 IL-2依賴型
          Source: Cell Type: lymphocyte cytotoxic T lymphocyte;
          Strain: C57BL/6
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Receptors: interleukin 2 (IL-2), expressed
          Comments: This line is a clone of cytotoxic T cells derived from a C57BL/6 mouse. The cells are dependent upon IL-2 for growth, and can be used to assay for IL-2.Immediay after thawing (or after overgrowth of a culture) the culture will appear to have few or no viable cells. This is normal.Depending upon the source and potency of the IL-2, it may take up to three weeks before the cells are ready to subculture.T-STIM with Con A (rat IL-2 culture supplement from Becton Dickinson) may be used or the rat factor may be prepared as described on the product sheet. The freshly prepared rat factor will usually produce more rapid growth.Tested and found negative for ectromelia virus (mousepox).
          Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: additional 2 mM L-glutamine; additional 1mM sodium pyruvate; adjust to a final concentration of 10% fetal bovine serum and 10% T-STIM with Con A. T-STIM is available from Becton Dickinson.
          Atmosphere: air, 95%; carbon dioxide (CO2), 5%
          Temperature: 37.0°C

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