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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機> CRL-2647 L Wnt-3A 小鼠皮下結(jié)締組織細(xì)胞

          [供應(yīng)]CRL-2647 L Wnt-3A 小鼠皮下結(jié)締組織細(xì)胞

          貨物所在地:上海上海市

          更新時間:2024-07-11 21:00:05

          有效期:2024年7月11日 -- 2025年1月11日

          已獲點擊:311

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          CRL-2647 L Wnt-3A 小鼠皮下結(jié)締組織細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!
          CRL-2647 L Wnt-3A 小鼠皮下結(jié)締組織細(xì)胞 的詳細(xì)介紹
          CRL-2647 L Wnt-3A 小鼠皮下結(jié)締組織細(xì)胞
          ATCC® Number: CRL-2647™    Price: $289.00
          Designations: L Wnt-3A
          Depositors:  R Nusse
          Biosafety Level: 1
          Shipped: frozen
          Medium & Serum: See Propagation
          Growth Properties: adherent
          Organism: Mus musculus (mouse)
          Morphology: fibroblast

          Source: Tissue: subcutaneous connective tissue; areolar and adipose
          Strain: C3H/An
          Cellular Products: Wnt-3A protein
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Age: 100 days
          Gender: male
          Comments: L-M(TK-) cells (ATCC CCL-1.3) were transfected with a Wnt-3A expression vector and stable clones were selected in medium containing G418.
          The Wnt-3A gene encodes a secreted glyoprotein with a variety of signaling effects. Wnt genes control many of the patterning and growth events during embryonic development.
          The cells secrete biologically active Wnt-3A protein. They are presently the best source for production of Wnt-3A conditioned medium.
          Since the conditioned medium contains other factors besides the Wnt-3A protein, it is necessary to control any experiments involving the Wnt-3A conditioned medium with control conditioned medium from the parental cell line (ATCC CRL-2648).
          Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 0.4 mg/ml G-418; fetal bovine serum to a final concentration of 10%.
          Atmosphere: air, 95%; carbon dioxide (CO2), 5%
          Temperature: 37.0°C
          Subculturing: Protocol:
          1. Remove and discard culture medium.
          2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
          3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
          5. Add appropriate aliquots of the cell suspension to new culture vessels.
          6. Incubate cultures at 37°C.

          Protocol for Wnt-3A conditioned medium:
          1. Split the cells 1:10 in 10 ml culture medium ( without G418 if conditioned medium is to be used with a cell line sensitive to G418) in 10 cm tissue culture dishes or T-75 flasks and let the cells grow for 4 days (approximay to confluency).2. Take off the medium and sterile filter. This is the first batch of medium.3. Add 10 ml fresh culture medium and culture for another 3 days.4. Take off the medium and sterile filter. This is the second batch of medium. Discard the cells, because they will be overgrown.5. Mix the first batch and second batch of medium 1:1. This is the Wnt-3A conditioned medium. It is stable at 4° C and can be frozen.
          Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
          Medium Renewal: Every 2 to 3 days
          Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
          Storage temperature: liquid nitrogen vapor phase
          Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
          recommended serum:ATCC 30-2020
          parental cell line:ATCC CRL-2648
          References: 90267: Willert K, et al. Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature 423: 448-452, 2003. PubMed: 12717451

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