immunoglobulin; monoclonal antibody; against human mesenchymal stem cells
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:
Yes
Comments:
Animals were immunized with human mesenchymal stem cells. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin.
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation:
culture medium 95%; DMSO, 5%
Related Products:
recommended serum:ATCC 30-2020 Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X
References:
22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996 49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137
Source: Cell Type: hybridoma: B lymphocyte; Cellular Products: immunoglobulin; monoclonal antibody; against human mesenchymal stem cells Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes Comments: Animals were immunized with human mesenchymal stem cells. Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells. The antibody reacts with mesenchymal stem cells from bone marrow and some tissues of mesenchymal origin. Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 85%; fetal bovine serum, 15% Temperature: 37.0°C Subculturing: Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml. Preservation: culture medium 95%; DMSO, 5% Related Products: recommended serum:ATCC 30-2020 Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 46-X References: 22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996 49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137
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