immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:
Animals were immunized with papain solubilized HLA A2 antigen. Spleen cells were fused with NS-1 myeloma cells. Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935] BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936] The antibody cross-reacts with cells from various primate species. Tested and found negative for ectromelia virus （mousepox）.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:
950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415 32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756 39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666
Source: Cell Type: hybridoma: B lymphocyte; Cellular Products: immunoglobulin; monoclonal antibody; against HLA A2, Aw69; cytotoxic Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with papain solubilized HLA A2 antigen. Spleen cells were fused with NS-1 myeloma cells. Mutation at position 107 of the alpha 2 domain of the HLA-A2 molecule results in partial loss of BB7.2 binding. [39935] BB7.2 and PA2.1 （ATCC HB-117） recognize overlapping epitopes on the alpha 2 domain of HLA-A2. [39936] The antibody cross-reacts with cells from various primate species. Tested and found negative for ectromelia virus （mousepox）. Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. References: 950: Parham P, Brodsky FM. Partial purification and some properties of BB7.2. A cytotoxic monoclonal antibody with specificity for HLA-A2 and a variant of HLA-A28. Hum. Immunol. 3: 277-299, 1981. PubMed: 7035415 32263: Cole DJ, et al. T-cell receptor repertoire in matched MART-1 peptide-stimulated peripheral blood lymphocytes and tumor-infiltrating lymphocytes. Cancer Res. 57: 5320-5327, 1997. PubMed: 9393756 39935: Santos-Aguado J, et al. Molecular characterization of serologic recognition sites in the human HLA-A2 molecule. J. Immunol. 141: 2811-2818, 1988. PubMed: 2459229 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666
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