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Designations:

LTPA

Depositors:

EH Leiter

Biosafety Level:

1

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Mus musculus （mouse）

Morphology:

epithelial

Source:

Organ: pancreas
Strain: LT/Sv
Disease: adenocarcinoma

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis:

Number of cells examined = 59; Modal Chromosome Number = 75 with a range of 65 to 79; Polyploidy Rate = 22%

Age:

12 month old

Gender:

female

Comments:

LTPA is an epithelial cell line derived in 1975 by Edward H. Leiter at The Jackson Laboratory, Bar Harbor, Maine from a spontaneous pancreatic adenocarcinoma taken from a 12 month old female Lt/Sv mouse.
A defective Polyoma virus infection of a pancreatic duct cell or its precursor appears to represent the neoplastic transforming event. LTPA cells carry a persistent Polyoma infection.
When injected subcutaneously into Swiss nu/nu mice, LTPA cells formed ductular structures, which were destroyed by inflammatory reactions within 3 weeks.
A culture submitted to the ATCC in May of 1998 was found to be contaminated with mycoplasma and progeny were cured by a 21-day treatment with BM Cycline.
The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.

Propagation:

ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

Subculturing:

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature （or at 37C） until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation:

culture medium 95%; DMSO, 5%

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