floating aggregates of round cells with some attached cells
Organism:
Homo sapiens （human）
Morphology:
epithelial
Source:
Organ: cecum Disease: colorectal adenocarcinoma
Cellular Products:
carcinoembryonic antigen （CEA）, 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil. The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended Medium Renewal: 3 times per week Remove medium, add fresh 0.25% trypsin, 0.03% EDTA solution for two minutes. Remove the trypsin and let the flask sit at room temperature for 10 minutes （or until the cells detach）. Add fresh medium, aspirate and dispense into new flasks.
Preservation:
Culture medium, 95%; DMSO, 5%
References:
23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
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