CRL-10317 MCF-10A 人正常乳腺上皮細(xì)胞，ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和*培養(yǎng)條件！
ATCC®Number:CRL-10317™ Price:$272.00
Designations:MCF 10ADepositors: Michigan Cancer FoundationBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens（human）Morphology:epithelial
Source:Organ:mammary gland; breast
Disease:fibrocystic disease
Cell Type:epithelialPermits/Forms:In addition to theMTAmentioned above, otherATCC and/or regulatory permitsmay be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Pleaseclick herefor information regarding the specific requirements for shipment to your location. Isolation:Isolation date:August 22, 1984Applications:transfection host （Roche FuGENE® Transfection Reagents）Tumorigenic:NoDNA Profile （STR）:Amelogenin: X
CSF1PO: 10,12
D13S317: 8,9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8,9.3
TPOX: 9,11
vWA: 15,17Isoenzymes:AK-1, 1 [23084]
ES-D, 1 [23084]
G6PD, B [23084]
GLO-I, 1-2 [23084]
PGM1, 1-2 [23084]
PGM3, 1 [23084]Age:36 yearsGender:femaleEthnicity:CaucasianComments:The MCF 10A cell line is a non-tumorigenic epithelial cell line. [21968]
The line was produced by long term culture in serum free medium with low Ca++ concentration. [21968]
MCF 10A was derived from adherent cells in the population. [21968]
Cells derived from a floating population are available （see MCF 10F, ATCC CRL-10318）. [21968]
The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. [21968]
They exhibit three dimensional growth in collagen, and form domes in confluent cultures. [21968]
Thus far, the cells have shown no signs of terminal differentiation or senescence.
The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor （EGF）. [21968]
By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. [23085]
They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. [23085]
The calcium content of the medium exerts a strong effect on the morphology of the cells. [22248]Propagation:ATCC complete growth medium:The base medium for this cell line （MEBM） along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 （gentamycin-amphotericin B mix） provided with the BEGM kit. To make the complete growth medium, you will need to add the following components to the kit （sold separay）:
100 ng/ml cholera toxin
Note: Do not filter complete medium
Temperature:37.0°C
Atmosphere:air, 95%; carbon dioxide （CO2）, 5%Subculturing:Protocol:Remove medium and rinse monolayer with PBS （ATCC Cat# 30-2200）.Add 3.0 ml 0.05% trypsin, 0.53 mM EDTA and incubate at 37C for 15 minutes. To neutralize trypsin, add 3 ml solution of 0.1% soybean trypsin inhibitor.Centrifuge cell suspension at 125 xg for 5 to 10 minutes.Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels.
Subc*tion Ratio:A subc*tion ratio of 1:3 to 1:4 is recommended
Medium Renewal:Every 2 to 3 daysPreservation:Freeze medium:Complete growth medium supplemented with 7.5% （v） DMSO
Storage temperature:liquid nitrogen vapor phaseRelated Products:derived from same individual:ATCCCRL-10318
derived from same individual:ATCCCRL-10780
derived from same individual:ATCCCRL-10781References:21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed:2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed:1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed:1697506
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