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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機(jī)> CRL-2285 Super Tube 狗腎細(xì)胞

          [供應(yīng)]CRL-2285 Super Tube 狗腎細(xì)胞

          貨物所在地:上海上海市

          更新時間:2024-08-29 21:00:06

          有效期:2024年8月29日 -- 2025年2月28日

          已獲點(diǎn)擊:318

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          CRL-2285 Super Tube 狗腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

          CRL-2285 Super Tube 狗腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

           

          CRL-2285 Super Tube 狗腎細(xì)胞 的詳細(xì)介紹

          ATCC® Number: CRL-2285™     
          Designations: Super Tube
          Depositors:  RJ Klebe
          Biosafety Level:1
          Shipped: frozen
          Medium & Serum: See Propagation
          Growth Properties:adherent
          Organism:Canis familiaris
          Morphology:epithelial
           
          Source:Organ: kidney
          Disease: normal
          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Age: adult
          Gender: female
          Comments:Super Tube is a canine epithelial-like cell line established by cloning from the MDCK (ATCC CCL-34) cell line that was derived from a kidney of an apparently normal adult female cocker spaniel.
          Super tube forms large numbers of tubules when maintained at high cell density (cells are reported to form tubules within 3 days when plated at 7 x 10exp5 cells/well of a 24 well plate).
          For tubule formation, the cells should be fed twice a day by a complete medium change.
          Super Tube has a much lower resting level of cyclic-AMP and responds to forskolin stimulation of adenylate cyclase less readily than the original cell line; it also has a lower transepithelal resistance than both Super Dome (ATCC CRL- 2286) and MDCK.
          Propagation: ATCC complete growth medium: These cells are grown in a medium containing a 1:1 mixture of Dulbeccos Modified Eagles Medium and Hams F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES, 0.5 mM sodium pyruvate, and 1.2 g/L sodium bicarbonate supplemented with 0.05 mM non-essential amino acids and 10 fetal bovine serum.
          Temperature: 37.0°C
          Subculturing: Protocol: Remove medium, and rinse with 0.25 trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
          Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:10 is recommended
          Medium Renewal: Every 2 to 3 days
          Preservation: Freeze medium: Complete growth medium 95; DMSO, 5
          Storage temperature: liquid nitrogen vapor temperature
          Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
          recommended serum:ATCC 30-2020
          parental cell line:ATCC CCL-34
          derived from same cell line:ATCC CRL-2286
          References:38915: Klebe RJ, et al. Cyclic-AMP deficient MDCK cells form tubules. J. Cell. Biochem. 59: 453-462, 1995. PubMed: 8749715

           

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