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        1. 上海復(fù)祥生物科技有限公司

          當(dāng)前位置:> 供求商機(jī)> VERO C1008 (E6)非洲綠猴腎細(xì)胞

          [供應(yīng)]VERO C1008 (E6)非洲綠猴腎細(xì)胞

          貨物所在地:上海上海市

          更新時(shí)間:2024-08-29 21:00:06

          有效期:2024年8月29日 -- 2025年2月28日

          已獲點(diǎn)擊:180

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          VERO C1008 (E6)非洲綠猴腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

          VERO C1008 (E6)非洲綠猴腎細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

          VERO C1008 (E6)非洲綠猴腎細(xì)胞 的詳細(xì)介紹

           

          ATCC® Number:CRL-1586™   Price:
          Designations:VERO C1008 [Vero 76, clone E6, Vero E6]
          Depositors: EM Earley
          Biosafety Level:1
          Shipped:frozen
          Medium & Serum:See Propagation
          Growth Properties:adherent
          Organism:Cercopithecus aethiops
          Morphology:epithelial
           
          Source:Organ: kidney
          Disease: normal
          Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
           
          Virus Susceptibility:Junin virus
          Machupo virus
          Lassa virus
          Marburg virus
          Zaire Ebola virus
          Comments:This is a clone of VERO 76 (ATCC CRL-1587).
          Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
          Temperature: 37.0°C
          Subculturing:Protocol:         
          1. Remove and discard culture medium.
          2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
          3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
            Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
          5. Add appropriate aliquots of the cell suspension to new culture vessels.
          6. Incubate cultures at 37°C.

          Subc*tion Ratio: A subc*tion ratio of 1:4 is recommended
          Medium Renewal: 2 to 3 times per week
          Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
          Storage temperature: liquid nitrogen vapor phase
          Doubling Time:22 hours
          Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
          recommended serum:ATCC 30-2020
          References:26095: Earley EM, Johnson KMThe lineage of Vero, Vero 76 and its clone C1008 in the United StatesIn: Earley EM, Johnson KMVero cells: origin, properties and biomedical applicationsTokyoChiba Univ.pp. 26-29, 1988
          32579: Schuster FL, Visvesvara GS. Axenic growth and drug sensitivity studies of Balamuthia mandrillaris, an agent of amebic meningoencephalitis in humans and other animals. J. Clin. Microbiol. 34: 385-388, 1996. PubMed: 8789020

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