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        1. 上海復祥生物科技有限公司

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          [供應]EBTr (NBL-4) 牛胚氣管細胞

          貨物所在地:上海上海市

          更新時間:2024-09-18 21:00:06

          有效期:2024年9月18日 -- 2025年3月18日

          已獲點擊:269

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          EBTr (NBL-4) 牛胚氣管細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

          EBTr (NBL-4) 牛胚氣管細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件

          EBTr (NBL-4) 牛胚氣管細胞 的詳細介紹


          ATCC® Number:  CCL-44™      
          Designations:  EBTr (NBL-4) 
          Depositors:   AJ Kniazeff, WA Nelson-Rees, NB Darby 
          Biosafety Level: 1 
          Shipped:  frozen 
          Medium & Serum:  See Propagation 
          Growth Properties: adherent
          Organism: Bos taurus (cow) 
          Morphology: fibroblast

           
          Source: Organ: trachea
          Disease: normal
          Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.     
           
          Virus Susceptibility: bovine viral diarrhea; infectious bovine rhinotracheitis; parainfluenzavirus 3; herpes simplex; reovirus 3; vesicular stomatitis (Ogden); vaccinia
          Virus Resistance: human adenovirus 5; poliovirus 2; coxsackievirus A9, B5 
          Reverse Transcript: negative 
          Age:  embryo 
          Gender:  male 
          Comments: The cells are negative for bovine viral diarrhea virus (BVDV). [22883]
          Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
          Temperature: 37.0°C    
          Subculturing:  Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
          Medium Renewal: Twice per week
          Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
          Add fresh culture medium, aspirate and dispense into new culture flasks.
          Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
          recommended serum:ATCC 30-2020       
          References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
          27802: Vassilev VB, et al. Authentic and chimeric full-length genomic cDNA clones of bovine viral diarrhea virus that yield infectious transcripts. J. Virol. 71: 471-478, 1997. PubMed: 8985373 
            
           

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