CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|,細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和*培養(yǎng)條件
CRL-1651 Cos-7 非洲綠猴sv40轉(zhuǎn)化的腎細胞 的詳細介紹
| ATCC® Number: | CRL-1651™ | Price: | $269.00 |
| Designations: | COS-7 | | Depositors: | Y Gluzman | | Biosafety Level: | 2 [Cells Contain SV-40 viral DNA sequences ] | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Cercopithecus aethiops | | Morphology: | fibroblast
 | | Source: | Organ: kidney
Cell Type: SV40 transformed | | Cellular Products: | T antigen | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | | Applications: | transfection host （Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents） | | Virus Susceptibility: | SV40 （lytic growth）; SV40 tsA209 at 40C; SV40 mutants with deletions in the early region | | Comments: | This is an African green monkey kidney fibroblast-like cell line suitable for transfection by vectors requiring expression of SV40 T antigen. This line contains T antigen, retains complete permissiveness for lytic growth of SV40, supports the replication of ts A209 virus at 40C, and supports the replication of pure populations of SV40 mutants with deletions in the early region.The line was derived from the CV-1 cell line （ATCC ® CCL-70?） by transformation with an origin defective mutant of SV40 which codes for wild type T antigen. | | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide （CO2）, 5%
Temperature: 37.0°C | | Subculturing: | Protocol: - Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% （w/v） Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed （usually within 5 to 15 minutes）.
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
|
|
14
