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詳細(xì)介紹
STREXS是一種模擬細(xì)胞生長(zhǎng)過(guò)程中機(jī)械應(yīng)力的裝置。張力腔室中的細(xì)胞被儀器拉伸或壓縮。施加在細(xì)胞上的機(jī)械應(yīng)力更好的模擬了自然動(dòng)態(tài)生理環(huán)境，可精準(zhǔn)控制施加在細(xì)胞上的應(yīng)力級(jí)別。
細(xì)胞應(yīng)力拉伸儀(STREX)的優(yōu)勢(shì)
× 精準(zhǔn)的應(yīng)力程序設(shè)計(jì)
× 高頻率和低頻率處可實(shí)現(xiàn)連續(xù)拉伸/壓縮
× 培養(yǎng)腔室所受應(yīng)力的均一性
× 橫向剪切力非常小
× 操作便捷
型號(hào) | ||||
項(xiàng)目 | ST-140-04 | ST-140-10 | ST-150 | ST-190-XY |
應(yīng)用范圍 | ? 細(xì)胞骨架重組 ? 細(xì)胞形態(tài)學(xué) ? 基因或蛋白表達(dá) ? 信號(hào)傳導(dǎo) ? 長(zhǎng)期性研究（幾小時(shí)到幾天） | ? 細(xì)胞骨架重組 ? 細(xì)胞形態(tài)學(xué) ? 離子調(diào)控 ? 鈣離子流入 ? 氮氧化合物的合成 ? 培養(yǎng)過(guò)程的實(shí)時(shí)監(jiān)測(cè) ? 短時(shí)間的過(guò)程研究（15-20min） | ||
腔室數(shù)目 | 6 | 5 | 1 | 1 |
腔室大小—培養(yǎng)面積 | 4cm2 | 10cm2 | 4cm2 | 4cm2 |
應(yīng)力方向 | 單軸拉伸 | 單軸拉伸 | 單軸拉伸 | 雙軸拉伸/壓縮 |
與顯微鏡兼容性 | - | - | 兼容Nikon, Olympus, 可選Zeiss, Leica | 兼容Nikon, Olympus, 可選Zeiss, Leica |
培養(yǎng)箱 | 標(biāo)準(zhǔn)培養(yǎng)箱 | 標(biāo)準(zhǔn)培養(yǎng)箱 | 標(biāo)準(zhǔn)培養(yǎng)箱 | 標(biāo)準(zhǔn)培養(yǎng)箱 |
程序個(gè)數(shù) | 64 | 64 | 64 | 64 |
Cells Stretching System
Manual Stretch System(for trials) STB-10
Stretching culture system of manual type
Conduct stretching/compression manually. Please use this system for evaluation purpose of automatic stretching systems(e.g. STB-140) before installation.
Users can share the chambers with STB-140.
Two models available in accordance with chamber sizes.
| System Configuration | System Specification |
|---|---|
| STB-10-04(for 4cm²) STB-10-10(for 10cm²) | Stretching ratio: up to 20%, 0.5mm stretching a turn of the dial(2.5% of stretching ratio for STB-10-04, 1.6% for STB-10-10). Note: Conduct culture putting the system into a culture dish. Stimulation direction: uniaxial |
Automatic Stretch System STB-140
Standard system with high versatility
The system has functionality to load stretching stimulation on cells while culturing them in CO² incubator.
● Functionality to stimulate multiple chambers simultaneously makes it easy to compare between samples.
● Install the main unit within CO² incubator, the controller outside.
● Stretch chamber mounting unit is removable, so users put it into clean bench and conduct aseptic work.
Preprogrammed one-chip microcomputer is embedded in the controller. While operation, users choose the stretching ratio and stretching frequency with DIP switch on the controller, and make the main unit to generate the desired stretching pattern.
The controller also has the functionality to control the water flow for refrigeration of the motor in the main unit.
| System Configuration | System Specification |
|---|---|
| Main Unit of Stretching System: STB-140-04(for 4cm²） STB-140-10(for 10cm²) STB-140-4*10(both for 4cm²/10cm²) Control Unit: Controls stretching patterns and refrigeration of the motor. Cables: The main unit and the controller are connected with the composite cable of signal cable and coolant cable(for the motor in the main unit). Coolant tank, Coolant tube | STB-140-04： 4cm² chambers up to 8 units STB-140-10： 10cm² chambers up to 6 units STB-140-4*10： 4cm² chambers up to 4 units 10cm² chambers up to 5 units Stretch chamber mounting unit is removable. (can be put a lid on with a culture dish) Stretching patterns: up to 64 patterns Stimulation direction: uniaxial Stretching ratio: up to 20% Driver(4.6kg) W270 × D350 × H130mm Controller(3.5kg) W320 × D220 × H125mm ※AC100V-240V 75W |
Stretch System for Microscopes STB-150
Installable on microscope stage
Enables to observe morphological changes and ionic behavior in real time, under stretching stimulation.
Install the main unit of the stretching system on microscope stages to load automatic stretching stimulation with the controller.
| System Configuration | System Specification |
|---|---|
| Main unit: normal type is compatible with Nikon, Olympus(compatibility with Zeiss and Leica is optional) Controlling unit: controls stretching patterns. | Compatible with 4cm² chamber Stimulation direction: uniaxial Stretching patterns: up to 64 patterns Stretching Stimulation Loading is up to 20 minutes continuously. (The system is without motor refrigeration unit. Long period operation results in the overheat of the motor. Use STB-140 for long period, continuous stimulation loading.) |
Stretch System for Microscopes STB-190-XY
Installable on microscope stages with functionality of biaxial stretching/compression
| System Specification |
|---|
| Compatible with 4cm² XY chamber Stimulation direction: XY biaxial, compatible with uniaxial stimulation Stretching patterns: up to 64 patterns Stretching Stimulation Loading is up to 20 minutes continuously. (The system is without motor refrigeration unit. Long period operation results in the overheat of the motor. Use STB-140 for long period, continuous stimulation loading.) |
Extention System for Microscopes(with Microincubator) STB-195
Installable on Microscope Stage(with Microincubator)
STB-150 equipped with Microincubator
| System Configuration | System Specification |
|---|---|
| Gas cylinder(Air 95% + CO² 5%) required | Temperature: 37℃ Humidity: Saturated humidity |
Accessories for Cultured Cells Stretch Systems
Culture Fluid Automatic Reflux System(Accessory for STB-140)SBB-100
Conducts full automatic replenishment and replacement of culture fluid while long-term Stretching Culture in CO² incubator
The system enables long-term culture under stretching stimulation. Newly-developed multi-channel bulb unit enables replenishment and draining culture fluid for all stretch chambers to keep liquid surface of culture fluid in those chambers.
Tubes and cables can be centrally let out from rubber plug for utility on the back of incubator so that the controllability on the front of incubator will not be compromised.
| System Configuration | System Specification |
|---|---|
| Controller Multi-channel bulb unit Replenishment pump for culture fluid(optional), Drain pump for culture fluid(optional) | Time and Volume of replenishment of culture fluid can be set up to 64 patterns with the controller. |
Electric Stimulation Synchronization Unit(Accessory for STB-140-10) SDB-50
Combined with STB-140-10, it loads stretching stimulation on cells synchronizing with electric stimulation.
| System Configuration | System Specification |
|---|---|
| Electrode lid Synchronization unit Note: electric stimulation amplifier(not included with the system) is required. | Stimulation frequency: 1-60cycles/min Stimulation duration: any durations(one-time stimulation to periodic stimulation for a month) Stretching ratio: up to 20% Stretching stimulation direction: uniaxial Stretchs up to 6 10cm² chambers once. |
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