詳細(xì)介紹
BioFlex細(xì)胞培養(yǎng)板---現(xiàn)貨供應(yīng)
Flexible-bottomed culture plate that works with the Flexcell® FX-5000™ Tension Systemto provide a mechanical load regimen to cells in monolayer (see Fig. 1 below).
- 6-well culture plate with total growth surface area of 57.75 cm2.
- Rubber membranes are optically clear for direct viewing of cells with an inverted or upright microscope.
- Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR). Learn more in Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment.
- Available individually or by the case of 40* plates.
Applications of BioFlex® Culture Plates
- Mechanical Load - use with the FX-5000™ Tension System to research the effects of equibiaxial tensile strain on cells in monolayer culture.
- Softer Substrate - provides a softer substrate for cell growth (when compared to standard plastic culture dishes) which better mimics in situ conditions for some cell types like trabecular meshwork cells (see Aga et al. Invest Ophthalmol Vis Sci 55(9):5497-509, 2014).
- Matrix Coated Surface - culture plate surfaces are coated with various matrix proteins, such as type I collagen and laminin, to better simulate in vivo conditions (see Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment).
- Traumatic Injury - can be used in studies looking at traumatic injury in astrocytes and neurons, where the injury is produced with a Cell Injury Controller that controls a burst of gas which deforms the BioFlex® membrane creating large biaxial strains (see Ellis et al. J Neurotrauma 12 325–339, 1995; Ahmed et al. J Neurochem 74(5):1951-1960, 2000; Augustine et al. Neuroscience 274:1-10, 2014).
Read more about Applying Mechanical Load to Cells in Monolayer Culture
Figure 1: Equibiaxial strain application (with a FX-5000™ Tension System) to cells plated in the well of a BioFlex® culture plate. |
五種不同包被的培養(yǎng)表面:Amino, Collagen (Type I or IV), Elastin, ProNectin (R GD), Laminin (YIGSR).
光學(xué)性能佳,自身熒光低。
當(dāng)與圓柱形加載平臺(tái)共同使用時(shí),可以對細(xì)胞進(jìn)行均勻的徑向和圓周應(yīng)力加載。
訂貨信息(請世聯(lián)博研公司)
產(chǎn)品編號(hào) 英文名稱
BF-3001U BioFlex Culture Plate — Untreated
BF-3001A BioFlex Culture Plate — Amino
BF-3001C BioFlex Culture Plate — Collagen Type I
BF-3001C(IV) BioFlex Culture Plate — Collagen Type IV
BF-3001E BioFlex Culture Plate — Elastin
BF-3001P BioFlex Culture Plate — ProNectin
BF-3001L BioFlex Culture Plate — Laminin
單向應(yīng)力細(xì)胞培養(yǎng)板UniFlex&tade; Culture Plates---現(xiàn)貨供應(yīng)
Uniflex細(xì)胞培養(yǎng)板和FX-5000T應(yīng)力加載系統(tǒng)配套使用,為單層細(xì)胞提供單軸向應(yīng)力。
UniFlex細(xì)胞培養(yǎng)板和FX-5000T應(yīng)力加載系統(tǒng)和TissueTrain細(xì)胞培養(yǎng)系統(tǒng)配套使用。
準(zhǔn)確測量應(yīng)力---負(fù)壓系統(tǒng)曲線,保證應(yīng)力的準(zhǔn)確性和可重復(fù)性。
單軸向應(yīng)力作用于寬0.6英寸x0.952英寸(3.68 cm2)的長方形區(qū)域中。
單軸向應(yīng)力變幅在+/-1.5%.
五種不同包被的培養(yǎng)表面:Amino, Collagen (Type I or IV) Elastin, ProNectin (RGD), Laminin (YIGSR)
為獲得準(zhǔn)確的單軸向應(yīng)力,必須和弧矩形應(yīng)力加載平臺(tái)配套使用。
訂貨信息(請世聯(lián)博研公司)
產(chǎn)品編號(hào) 英文名稱
UF-4001U UniFlex Culture Plate — Untreated
UF-4001A UniFlex Culture Plate — Amino
UF-4001C UniFlex Culture Plate — Collagen Type I
UF-4001C(IV) UniFlex Culture Plate — Collagen Type IV
UF-4001E UniFlex Culture Plate — Elastin
UF-4001P UniFlex Culture Plate — ProNectin
UF-4001L UniFlex Culture Plate — Laminin
Flexible-bottomed culture plate that works with the FX-5000™ Tension System to provide uniaxial strain to cells in monolayer culture.
- Uniaxial strain applied to a strain field represented by a 0.6" wide x 0.952" (3.68 cm2) long centrally located rectangular region.
- Arctangle™ Loading Stations™ are required for correct application of uniaxial strain (see Fig. 1 below; additional information located in the Tech Report).
- Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR). Learn more in Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment.
- Available individually or by the case of 40*.
Applications of UniFlex® Culture Plates
- Mechanical Load - use with the FX-5000™ Tension System to research the effects of uniaxial tensile strain on cells in monolayer culture.
- Softer Substrate - provides a softer substrate for cell growth when compared to standard plastic culture dishes, which better mimics in situ conditions for some cell types like trabecular meshwork cells (see Aga et al. Invest Ophthalmol Vis Sci 55(9):5497-509, 2014).
- Matrix Coated Surface - culture plate surfaces are coated with various matrix proteins to better simulate in vivo conditions (see Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment).
- Microgrooved Channels - plates have been modified to hold microgrooved channels molded with PDMS. Plates can then be mechanically loaded with the FX-5000™ Tension System, if desired (seeJones et al. J Biomech 38(8):1653-1664, 2005).
Read more about Applying Mechanical Load to Cells in Monolayer Culture
Figure 1: A) Arctangle® loading post. B) UniFlex® well. C) UniFlex® Culture Plate resting atopArctangle® Loading Station™. |