詳細(xì)介紹
Well Dynamic Confiner
動(dòng)態(tài)細(xì)胞封閉試劑盒由1×Elveflow真空和安培組成。壓力發(fā)生器和1xelveflow AF1雙真空&安培;壓力控制器10 x限制載玻片10 mm(直徑)玻璃載玻片/蓋玻片,在PDMS(柱子)中具有微結(jié)構(gòu),使限制成為可能。可用約束高度從1、2、3,20嗯(選擇3高度集成工具包)3 x 4 dcell動(dòng)態(tài)限制(PDMS吸盤)1 x安全柜(保護(hù)真空泵)1 x工具包的管子和連接器,如果你想讓你的細(xì)胞或幻燈片膠粘劑(可選):用于細(xì)胞粘附的細(xì)胞外基質(zhì)蛋白(如纖維連接蛋白)在正確的緩沖溶液中,準(zhǔn)備在載玻片上粘附的抗粘附分子(聚乙二醇)
Well Dynamic Confiner
The dynamic cell confiner kit is composed by:
- 1 x Elveflow Vacuum & Pressure Generator and 1 x Elveflow AF1 DUAL – Vacuum & Pressure Controller
- 10 x Confinement Slides – 10 mm (diameter) glass slides/cover slips with micro-structures in PDMS (pillars) that enable the confinement.
Available confinement heights – from 1, 2, 3, … up to 20 um (choose up to 3 heights to integrate your kit) - 3 x 4Dcell Dynamic Confiner™ (PDMS Suction Cup)
- 1 x security tank (to preserve the vacuum pump)
- 1 x Kit of Tubes and Connectors
If you want to make your slides adhesive to your cells or not (optional):
- Aliquots of extracellular matrix protein for cell adhesion (for example, fibronectin) in the right buffer solution
- Aliquots of anti-adhesive molecule (poly-ethylene glycol) ready to bind on the slides