詳細(xì)介紹
UV Printer微模式打印:與我們的產(chǎn)品相關(guān)的UV打印機(jī)的主要應(yīng)用是將微模式打印到玻璃基板上(無(wú)論是微模式的幻燈片還是微模式的盤(pán)子)。燈和一切你需要得到的微模式上的幻燈片是提供了微模式制作工具包。下面簡(jiǎn)單的程序?qū)⒄f(shuō)明UV打印機(jī)的使用。先,在玻璃基板上涂上防粘劑,如聚乙二醇(PEG)(可提供預(yù)涂玻片)。為了使表面具有圖案,在基板上放置微圖案光掩膜。它將定義使細(xì)胞粘附的區(qū)域。然后,深紫外光的高能光會(huì)損壞打印圖案的暴露區(qū)域。后,細(xì)胞外基質(zhì)(ECM)蛋白被裝載在處理表面。圖案涂層的防污效果將使ECM只附著在受損區(qū)域。因此,細(xì)胞只會(huì)在受限制的區(qū)域粘附和增殖。UV Printer
Printing the micropatterns:
The main application of the UV Printer related with our products is to print the micropatterns onto the glass substrate (either a Micropatterned slide or aMicropatterned dish).The lamp and everything you need to get micropatterns on slides is provided with the Micropatterns Fabrication Kit. The following brief procedure will illustrate the use of the UV Printer.
First, the glass substrate is coated with an anti-adhesive agent, e.g. Polyethylene glycol (PEG) (pre-coated slides can be provided).
To pattern the surface, a Micropatterning Photomask is placed over the substrate. It will define the areas that will enable the cells to be adherent.
Then, the high energy light of the deep UVs will damage the exposed areas printing the patterns.
Finally, extra cellular matrix (ECM) proteins are loaded over the treated surface. The anti-fouling effect of the patterned coating will make the ECM attach only on the damaged areas. Thus, the cells will adhere and multiply only over the restricted areas.