24-roundwell microplate 300 rpm

24-roundwell microplate 300 rpm

24圓孔微板孔內(nèi)流體力學(xué)

對(duì)于24個(gè)圓孔微量滴定板，使用0.5至1毫升的培養(yǎng)體積，大多數(shù)情況下，在25毫米振幅的振動(dòng)篩上以300轉(zhuǎn)/分（視頻在左側(cè)）或在50毫米振幅的振動(dòng)篩上以225轉(zhuǎn)/分（視頻在右側(cè)）進(jìn)行培養(yǎng)。在兩種情況下（培養(yǎng)體積為0.75毫升），氧遷移率約為30毫摩爾O2 L-1 H-1（對(duì)應(yīng)于150 H-1的KLA值）。對(duì)于以下（放慢）的高速視頻，我們使用來自康寧的24個(gè)孔板，其廣泛用于細(xì)菌和細(xì)胞的培養(yǎng)；也因?yàn)樗鼈冊(cè)谑澜绺鞯囟加胁煌谋砻嫣幚恚▽?dǎo)致不同的結(jié)合特性）和伽瑪輻照。

建議（默認(rèn)）條件：1:25 mm搖床振幅，300轉(zhuǎn)/分（在NB上培養(yǎng)大腸桿菌，30次減速）0.5毫升0.75毫升1毫升

Hydrodynamics inside wells of a 24-roundwell microplate
For 24-roundwell microtiter plates, culture volumes between 0.5  and 1 ml are used, mostly either at 300 rpm on a shaker with a 25 mm amplitude (video on the left) or at 225 rpm on a shaker with a 50 mm amplitude (video on the right).Under both conditions (at a culture volume of 0.75 ml), oxygen-transfer rates approximate 30 mmol O2 l-1 h-1  (corresponding to kLa values of 150 h-1).For the (slowed-down) high-speed videos below we used 24-well plates from Corning, which are widely used for the c*tion of bacteria and cells, also because they are worldwide available with various surface treatements (causing different binding characteristics) and gamma irradiated.
Adviced (default) condtion 1:25 mm shaker amplitude, 300 rpm(E. coli culture on NB, 30 times slowed down)    0.5 ml       0.75 ml      1 ml

 注：楊清辰發(fā)布