96-square half deepwell microplate 225

96-square half deepwell microplate 225

96方半深孔微板孔內(nèi)流體力學(xué)

對(duì)于96平方的半深孔微量滴定板（CR1496c，專門用于培養(yǎng)目的），通常使用0.25-0.35毫升的培養(yǎng)體積，在50毫米振幅的振動(dòng)篩上。振蕩頻率取決于培養(yǎng)液的體積，對(duì)于0.25或0.3ml的培養(yǎng)液，一般采用225或250轉(zhuǎn)/分，這種微孔板的氧傳遞速率（OTR）尚未測(cè)定。從96平方深井板（Duetz等人，2000，應(yīng)用和環(huán)境微生物學(xué)66:2641-2646）獲得的數(shù)據(jù)推斷，在225轉(zhuǎn)/分、40 mmol O2 l-1 h-1（對(duì)應(yīng)于200 h-1的kLa值）下，估計(jì)的OTR為250 ul。這樣的OTR對(duì)應(yīng)于在ErnMeYER振動(dòng)篩燒瓶中的可實(shí)現(xiàn)（總瓶體積的培養(yǎng)體積10%，300轉(zhuǎn)/分），以確保*混合到底部（無死區(qū)），然而，建議使用250轉(zhuǎn)/分的振蕩頻率而不是225轉(zhuǎn)/分。在不使用高于0.25毫升的培養(yǎng)液的情況下（見本頁底部的兩個(gè)視頻蒸餾器。如果只有25毫米振幅的振動(dòng)篩可用，則可以在培養(yǎng)液不接觸蓋子的情況下使用更高的振動(dòng)頻率（275或300轉(zhuǎn)/分），還報(bào)告了在300轉(zhuǎn)/分（培養(yǎng)體積為0.25毫升）下?lián)u動(dòng)以獲得良好的結(jié)果（流體力學(xué)和氧轉(zhuǎn)移速率與500毫米/225轉(zhuǎn)/分相當(dāng)）

建議（默認(rèn)）條件：2～50毫米振動(dòng)篩振幅，225轉(zhuǎn)/分鐘（大腸桿菌培養(yǎng)NB，20倍減慢）0.25毫升0.3毫升0.35毫升（*混合至0.30毫升培養(yǎng)體積）

 Hydrodynamics inside wells of a 96-square half deepwell microplate
For 96-square half deepwell microtiter plates (CR1496c, specifically designed for c*tion purposes), culture volumes between 0.25 ml and  0.35 ml are typically used, preferably on a shaker with a 50 mm amplitude. The optimal shaking frequency depends on the culture volume.For 0.25  or 0.3 ml cultures, 225 or 250 rpm is generally used.Oxygen transfer rates (OTR) for this kind of microplate have not been measured yet. Extrapolation from the data obtained with 96-square deepwell plates (Duetz et al., 2000, Applied and Environmental Microbiology 66:2641-2646) leads to an estimated OTR at 250 ul at 225 rpm of 40 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), To ensure full mixing down to the bottom (no dead zones), however, it may be advisable to use shaking frequencies of 250 rpm instead of 225 rpm. Even higher shaking frequencies (275 or 300 rpm) may be applied without the culture fluid touching upon the cover, on the condition that no higher culture volumes than 0.25 ml are used (see the bottom two video stills on this page.If only a shaker with a 25 mm amplitude is avaialble, shaking at 300 rpm (and a culture volume of 0.25 ml) is also reported to give good results (hydrodynamics and oxygen transfer rates comparable with 500 mm/225 rpm)
Adviced (default) condtion 2:50 mm shaker amplitude, 225 rpm(E. coli culture on NB, 20 times slowed down)    0.25 ml       0.3 ml      0.35 ml   (full mixing up to 0.30 ml culture volume)

 注：楊清辰發(fā)布