6-square deepwell microplate

6-square deepwell microplate

96平方深井孔板孔內(nèi)流體力學(xué)

對(duì)于96平方深孔微量滴定板，通常使用0.5毫升至0.75毫升的培養(yǎng)體積，在50毫米振幅的振動(dòng)篩上以300轉(zhuǎn)/分的速度進(jìn)行培養(yǎng)（右圖為照片和視頻）。對(duì)于0.5毫升的培養(yǎng)物，這些振動(dòng)條件導(dǎo)致38 mmol O2 l-1 h-1的氧轉(zhuǎn)移速率（OTR）（對(duì)應(yīng)于200 h-1的kLa值）。這樣的OTR在其轉(zhuǎn)彎中對(duì)應(yīng)于在ErnMEMEER振動(dòng)篩燒瓶中的可實(shí)現(xiàn)（總瓶體積的培養(yǎng)體積10%，300轉(zhuǎn)/分），并且進(jìn)一步確保混合到底部（無死區(qū)）。后者特別適用于大而重的細(xì)胞（如酵母菌和CHO細(xì)胞），這些細(xì)胞往往在死區(qū)沉淀。有些組使用0.6毫升的培養(yǎng)液，而不是0.5毫升，這仍然導(dǎo)致可接受的混合程度。

Hydrodynamics inside wells of a 96-square deepwell microplate
For 96-square deepwell microtiter plates, culture volumes between 0.5 ml and  0.75 ml are typically used, preferably at 300 rpm on a shaker with a 50 mm amplitude (photo and video on the right).For 0.5 ml cultures, these shaking conditions lead to oxygen transfer rates (OTR) of 38 mmol O2 l-1 h-1  (corresponding to kLa values of 200 h-1). Such an OTR - in its turn - corresponds to the high end achievable in Erlenmeyer shaker flasks (culture volume 10% of total flask volume, 300 rpm), and furthermore ensures mixing down to the bottom (no dead zones). The latter is especially relevant  for large and heavy cells (such as yeasts and CHO-cells) that tend to precipitate in dead zones. Some groups use culture volumes of 0.6 ml instead of 0.5 ml, which still results in an acceptable degree of mixing.
25 mm shaker amplitude•300 rpm(full mixing up to 0.25 ml culture volume)

注：楊清辰發(fā)布