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          應(yīng)用領(lǐng)域 醫(yī)療衛(wèi)生,生物產(chǎn)業(yè)    
          細(xì)胞剪切力學(xué)實(shí)驗(yàn)系,flexcell str-4000,細(xì)胞切應(yīng)力實(shí)驗(yàn)系統(tǒng),藥代動力學(xué)實(shí)驗(yàn)裝置,可提供穩(wěn)流式切應(yīng)力、脈沖式切應(yīng)力或者往返式切應(yīng)力多種流場刺激,在實(shí)現(xiàn)細(xì)胞在流體切應(yīng)力的同時抻拉,實(shí)時觀察細(xì)胞在多力混合加載刺激下的變化反應(yīng),細(xì)胞量大,便于后期分析,便于后期分析細(xì)胞中基因的表達(dá)(mRNA檢測),以及使用流式細(xì)胞儀、基因芯片等進(jìn)行分析,產(chǎn)品成熟度與使用案例:國外內(nèi)有數(shù)千篇文獻(xiàn)

          詳細(xì)介紹

          細(xì)胞剪切力學(xué)實(shí)驗(yàn)系統(tǒng),flexcell str-4000

          美國flexcell品牌,型號:str-4000

          細(xì)胞剪切力學(xué)實(shí)驗(yàn)系統(tǒng),可提供穩(wěn)流式切應(yīng)力、脈沖式切應(yīng)力或者往返式切應(yīng)力多種流場刺激

          細(xì)胞剪切力學(xué)實(shí)驗(yàn)系,flexcell str-4000,細(xì)胞切應(yīng)力實(shí)驗(yàn)系統(tǒng),藥代動力學(xué)實(shí)驗(yàn)裝置,可提供穩(wěn)流式切應(yīng)力、脈沖式切應(yīng)力或者往返式切應(yīng)力多種流場刺激,在實(shí)現(xiàn)細(xì)胞在流體切應(yīng)力的同時抻拉,實(shí)時觀察細(xì)胞在多力混合加載刺激下的變化反應(yīng),細(xì)胞量大,便于后期分析,便于后期分析細(xì)胞中基因的表達(dá)(mRNA檢測),以及使用流式細(xì)胞儀、基因芯片等進(jìn)行分析,產(chǎn)品成熟度與使用案例:國外內(nèi)有數(shù)千篇文獻(xiàn)

          Flexflow單通道平行板流室系統(tǒng)提供流體切應(yīng)力同時抻拉細(xì)胞


          FlexcellFlexFlow顯微切應(yīng)力加載設(shè)備(SHEAR Stress device)

          • 可以在提供流體切應(yīng)力的同時抻拉細(xì)胞,測試血管和結(jié)綈組織細(xì)胞對液體流動的實(shí)時反應(yīng)。
          • 為培育在StageFlexer硅膠模表面或者基質(zhì)蛋白包被的細(xì)胞培養(yǎng)片上的細(xì)胞提供切應(yīng)力。
          • 使用FX-5000T應(yīng)力加載系統(tǒng)抻拉細(xì)胞,并且可以在實(shí)驗(yàn)前,實(shí)驗(yàn)中或者實(shí)驗(yàn)后提供切應(yīng)力。
          • 計算機(jī)控制蠕動泵,調(diào)節(jié)切應(yīng)力大小,從0-35 dynes/cm2
          • 使用標(biāo)準(zhǔn)正立式顯微鏡實(shí)時觀察細(xì)胞在切應(yīng)力下的反應(yīng)。
          • 檢測細(xì)胞在流體作用下的排列反應(yīng)。
          • 加力同時實(shí)時檢測在液體切應(yīng)力下各種激活劑/抑制劑對細(xì)胞反應(yīng)的影響。使用熒光團(tuán)例如FURA-2檢測細(xì)胞內(nèi)[Ca2+]ic或者其它離子對切應(yīng)力反應(yīng)

          典型應(yīng)用文獻(xiàn):

          1. Archambault JM, Elfervig MK, Tsuzaki M, Herzog W, Banes AJ. Shear stress response of rabbit tendon cells is serum dependent. Proceedings of the Eleventh Canadian Society for Biomechanics Conference, 181, 2000.
          2. Archambault JM, Elfervig-Wall MK, Tsuzaki M, Herzog W, Banes AJ. Rabbit tendon cells produce MMP-3 in response to fluid flow without significant calcium transients. J Biomech 35(3):303-309, 2002.

          3. Clark PR, Jensen TJ, Kluger MS, Morelock M, Hanidu A, Qi Z, Tatake RJ, Pober JS. MEK5 is activated by shear stress, activates ERK5 and induces KLF4 to modulate TNF responses in human dermal microvascular endothelial cells. Microcirculation 18(2):102-117, 2011.
          4. de Castro LF, Maycas M, Bravo B, Esbrit P, Gortazar A. VEGF receptor 2 (VEGFR2) activation is essential for osteocyte survival induced by mechanotransduction. J Cell Physiol 230(2):278-85, 2015.
          5. Eifler RL, Blough ER, Dehlin JM, Haut Donahue TL. Oscillatory fluid flow regulates glycosaminoglycan production via an intracellular calcium pathway in meniscal cells. J Orthop Res 24(3):375-384, 2006.
          6. Elfervig M, Francke E, Archambault J, Herzog W, Tsuzaki M, Bynum D, Brown TD, Banes AJ. Fluid-induced shear stress activates human tendon cells to signal through multiple Ca2+ dependent pathways [abstract]. Transactions of the 46th Annual Meeting of the Orthopaedic Research Society 25:179, 2000.
          7. Elfervig M, Lotano M, Tsuzaki M, Faber J, Banes A J. Fluid-induced shear stress modulates Cx-43 expression in avian tendon cells but does not induce a Ca2+ signal [abstract]. Transactions of the 47th Annual Meeting of the Orthopaedic Research Society 26:570, 2001.
          8. Elfervig MK, Minchew JT, Francke E, Tsuzaki M, Banes AJ. IL-1? sensitizes intervertebral disc annulus cells to fluid-induced shear stress. J Cell Biochem 82(2):290-298, 2001.
          9. Finley MJ, Rauova L, Alferiev IS, Weisel JW, Levy RJ, Stachelek SJ. Diminished adhesion and activation of platelets and neutrophils with CD47 functionalized blood contacting surfaces. Biomaterials 33(24):5803-5811, 2012.
          10. Francke E, Banes A, Elfervig M, Brown T, Bynum D. Fluid-induced shear stress increases [Ca2+]ic in cultured human tendon epitenon cells [abstract]. Transactions of the 46th Annual Meeting of the Orthopaedic Research Society 25:638, 2000.
          11. Francke E, Elfervig MK, Sood A, Brown TD, Bynum DK, Banes AJ. Fluid-induced shear stress stimulates Ca2+ signaling in human epitenon cells [abstract]. 1999 Advances in Bioengineering, J.S. Wayne, ed. American Society of Mechanical Engineers: New York, 1999.
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          14. Glossop JR, Hidalgo-Bastida LA, Cartmell SH. Fluid shear stress induces differential gene expression of leukemia inhibitory factor in human mesenchymal stem cells. J Biomat Tiss Eng 1:166-176, 2011.
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          16. Grabias BM, Konstantopoulos K. Epithelial-mesenchymal transition and fibrosis are mutually exclusive reponses in shear-activated proximal tubular epithelial cells. FASEB J 26(10):4131-41, 2012.
          17. Guan PP, Yu X, Guo JJ, Wang Y, Wang T, Li JY, Konstantopoulos K, Wang ZY, Wang P. By activating matrix metalloproteinase-7, shear stress promotes chondrosarcoma cell motility, invasion and lung colonization. Oncotarget 6(11):9140-59, 2015.
          18. Hamamura K, Zhang P, Zhao L, Shim JW, Chen A, Dodge TR, Wan Q, Shih H, Na S, Lin CC, Sun HB, Yokota H. Knee loading reduces MMP13 activity in the mouse cartilage. BMC Musculoskelet Disord 14(1):312, 2013.
          19. Hosoya T, Maruyama A, Kang MI, Kawatani Y, Shibata T, Uchida K, Warabi E, Noguchi N, Itoh K, Yamamoto M. Differential responses of the Nrf2-Keap1 system to laminar and oscillatory shear stresses in endothelial cells. J Biol Chem 280(29):27244-27250, 2005.
          20. Jaitovich A, Mehta S, Na N, Ciechanover A, Goldman RD, Ridge KM. Ubiquitin-proteasome-mediated degradation of keratin intermediate filaments in mechanically stimulated A549 cells. J Biol Chem 283(37):25348-25355, 2008.
          21. Kamel MA, Picconi JL, Lara-Castillo N, Johnson ML. Activation of β-catenin signaling in MLO-Y4 osteocytic cells versus 2T3 osteoblastic cells by fluid flow shear stress and PGE2: implications for the study of mechanosensation in bone. Bone 47(5):872-881, 2010.
          22. Lee CY, Hsu HC, Zhang X, Wang DY, Luo ZP. Cyclic compression and tension regulate differently the metabolism of chondrocytes. J Musculoskeletal Res 9(2):59-64, 2005.

          23. Li M, Liu X, Zhang Y, Di M, Wang H, Wang L, Chen Y, Liu X, Cao X, Zeng R, Zhang Y, Zhang M. Upregulation of Dickkopf1 by oscillatory shear stress accelerates atherogenesis. J Mol Med (Berl) 94(4):431-41, 2016.
          24. Liao C, Cheng T, Wang S, Zhang C, Jin L, Yang Y. Shear stress inhibits IL-17A-mediated induction of osteoclastogenesis via osteocyte pathways. Bone 101:10-20, 2017.
          25. Liu J, Bi X, Chen T, Zhang Q, Wang SX, Chiu JJ, Liu GS, Zhang Y, Bu P, Jiang F. Shear stress regulates endothelial cell autophagy via redox regulation and Sirt1 expression. Cell Death Dis 6:e1827, 2015.
          26. Malone AM, Batra NN, Shivaram G, Kwon RY, You L, Kim CH, Rodriguez J, Jair K, Jacobs CR. The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts. Am J Physiol Cell Physiol 292(5):C1830-C1836, 2007.
          27. Maycas M, Ardura JA, de Castro LF, Bravo B, Gortázar AR, Esbrit P. Role of the parathyroid hormone type 1 receptor (PTH1R) as a mechanosensor in osteocyte survival. J Bone Miner Res 30(7):1231-44, 2015.
          28. Maycas M, Bravo-Molina B, Fernández de Castro L, Pozuelo JM, Forriol F, P Esbrit, Rodríguez de Gortázar A. High glucose alters the antiapoptotic response to mechanical stimulation in MLO-Y4 osteocytic cells. Trauma Fund MAPFRE 25(2):97-100, 2014.
          29. Metaxa E, Meng H, Kaluvala SR, Szymanski MP, Paluch RA, Kolega J. Nitric oxide-dependent stimulation of endothelial cell proliferation by sustained high flow. Am J Physiol Heart Circ Physiol 295(2):H736-H742, 2008.
          30. Ni J, Waldman A, Khachigian LM. c-Jun regulates shear- and injury-inducible Egr-1 expression, vein graft stenosis after autologous end-to-side transplantation in rabbits, and intimal hyperplasia in human saphenous veins. J Biol Chem 285(6):4038-4048, 2010.
          31. Qi J, Chi L, Faber J, Koller B, Banes AJ. ATP reduces gel compaction in osteoblast-populated collagen gels. J Appl Physiol 102(3):1152-60, 2007.
          32. Radel C, Carlile-Klusacek M, Rizzo V. Participation of caveolae in ?1 integrin-mediated mechanotransduction. Biochem Biophys Res Commun 358(2):626-631, 2007.
          33. Radel C, Rizzo V. Integrin mechanotransduction stimulates caveolin-1 phosphorylation and recruitment of Csk to mediate actin reorganization. Am J Physiol Heart Circ Physiol 288(2):H936-H945, 2005.
          34. Ridge KM, Linz L, Flitney FW, Kuczmarski ER, Chou YH, Omary MB, Sznajder JI, Goldman RD. Keratin 8 phosphorylation by protein kinase C ? regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells. J Biol Chem 280(34):30400-30405, 2005.
          35. Riehl BD, Lee JS, Ha L, Kwon IK, Lim JY. Flowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing. PLoS One 12(2):e0171857, 2017.
          36. Riehl BD, Lee JS, Ha L, Lim JY. Fluid-flow-induced mesenchymal stem cell migration: role of focal adhesion kinase and RhoA kinase sensors. J R Soc Interface 12(107), 2015. pii: 20150300.
          37. Rosser J, Bonewald LF. Studying osteocyte function using the cell lines MLO-Y4 and MLO-A5. Methods Mol Biol 816:67-81, 2012.
          38. Shim JW, Hamamura K, Chen A, Wan Q, Na S, Yokota H. Rac1 mediates load-driven attenuation of mRNA expression of nerve growth factor ? in cartilage and chondrocytes. J Musculoskelet Neuronal Interact 13(3):372-9, 2013.
          39. Siu KL, Gao L, Cai H. Differential roles of /NOXO1 and NOX2/p47phox in mediating endothelial redox responses to oscillatory and unidirectional laminar shear stress. J Biol Chem 291(16):8653-62, 2016.
          40. Sivaramakrishnan S, DeGiulio JV, Lorand L, Goldman RD, Ridge KM. Micromechanical properties of keratin intermediate filament networks. PNAS 105(3):889-894, 2008.
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          FLEXCELL® INTERNATIONAL CORPORATION
          76
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