Flexflow單通道平行板流室系統(tǒng)提供流體切應(yīng)力同時(shí)抻拉細(xì)胞
1）FlexFlow顯微切應(yīng)力加載設(shè)備（SHEAR Stress device）
2、流體剪切應(yīng)力培養(yǎng)載片如下圖：
細(xì)胞培養(yǎng)載片包括顯微鏡載(物)片和蓋玻片兩種產(chǎn)品，表面經(jīng)過特殊處理，適合于細(xì)胞的貼壁與生長。
兩種規(guī)格：75 mm x 25 mm x 1.0 mm ,75 mm x 24 mm x 0.2 mm 。
細(xì)胞培養(yǎng)載片的邊緣涂有1.0 mm寬的特氟隆邊框(Teflon)，可以有效控制細(xì)胞生長在切應(yīng)力加載區(qū)域。
自身熒光低，光學(xué)性能佳。
不同包被的培養(yǎng)表面提高細(xì)胞的貼壁與生長。
五種不同包被的培養(yǎng)表面：Amino, Collagen (Type I or IV) Elastin, ProNectin (RGD), Laminin (YIGSR).
所以產(chǎn)品都是無菌獨(dú)立包裝，僅供一次性使用。
75mm x 24mm x 0.2mm 和 FlexFlow配套使用
產(chǎn)品編號(hào) 英文名稱
FFCS-U Culture Slips — Untreated
FFCS-A Culture Slips — Amino
FFCS-C Culture Slips — Collagen Type I
FFCS-C(IV) Culture Slips — Collagen Type IV
FFCS-E Culture Slips — Elastin
FFCS-P Culture Slips — ProNectin
FFCS-L Culture Slips — Laminin
3）微流納流HiQ Flowmate微流體控制器
雙注射泵可以在微升、納升、微微升水平上控制液流.雙注射泵，獨(dú)立的液流控制系統(tǒng)。
傳送，穩(wěn)定的流速
可控流速范圍1.2pL/ min-260.6ml/min
提供不同流速模型：穩(wěn)定型，脈沖型，連續(xù)型，截流型和震蕩型；
可進(jìn)行循環(huán)，連續(xù)的液流控制；同時(shí)運(yùn)行不同的流速模型；
內(nèi)置閥門控制液流模式；
機(jī)載計(jì)算器用于流量、流時(shí)、流速、剪切力的計(jì)算；
高分辨率、觸屏控制。
用戶友好的圖標(biāo)驅(qū)動(dòng)程序；
便于泵和芯片對(duì)接的生物芯片支架；根據(jù)現(xiàn)有流速有三種不同的機(jī)型；
多種應(yīng)用程序：
液體稀釋，配給及注射器；
動(dòng)物實(shí)驗(yàn)中的藥物注射和體液抽?。?/p>
施加液流剪切力；
微流體和納流體實(shí)驗(yàn)；
混合、分流液體；
震蕩型液流的控制需要iHIQ Flowmate二級(jí)閥門配件
系統(tǒng)完整結(jié)構(gòu)圖：
1.可以在提供流體切應(yīng)力的同時(shí)抻拉細(xì)胞，測(cè)試血管和結(jié)綈組織細(xì)胞對(duì)液體流動(dòng)的實(shí)時(shí)反應(yīng)。
2.為培育在StageFlexer硅膠模表面或者基質(zhì)蛋白包被的細(xì)胞培養(yǎng)片上的細(xì)胞提供切應(yīng)力。
3.使用FX-5000T應(yīng)力加載系統(tǒng)抻拉細(xì)胞，并且可以在實(shí)驗(yàn)前，實(shí)驗(yàn)中或者實(shí)驗(yàn)后提供切應(yīng)力
4.計(jì)算機(jī)控制蠕動(dòng)泵，調(diào)節(jié)切應(yīng)力大小，從0-35 dynes/cm2
5.使用標(biāo)準(zhǔn)正立式顯微鏡實(shí)時(shí)觀察細(xì)胞在切應(yīng)力下的反應(yīng)。
6.檢測(cè)細(xì)胞在流體作用下的排列反應(yīng)。
7.加力同時(shí)實(shí)時(shí)檢測(cè)在液體切應(yīng)力下各種激活劑/抑制劑對(duì)細(xì)胞反應(yīng)的影響。使用熒光團(tuán)例如FURA-2檢測(cè)細(xì)胞內(nèi)[Ca2+]ic或者其它離子對(duì)切應(yīng)力反應(yīng)。（可以與str-4000六通道切應(yīng)力系統(tǒng)配套使用）
FLEXFLOW AND STREAMER FLUID SHEAR STRESS SYSTEMS
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FlexFlow系統(tǒng)包括：
FlexFlow裝置；StreamSoft軟件
FlexFlow快拆接頭、膠管、FlexFlow 旁路連接器
MASTERFLEX L/S型號(hào)7550-10蠕動(dòng)泵及配套線纜、連接管
2個(gè)穩(wěn)流器；硅潤滑劑
FX -5000 張力系統(tǒng)適配器
顯微鏡適應(yīng)性FlexFlow底座
快速鏈接細(xì)胞培養(yǎng)基瓶；一個(gè)快速鏈接真空瓶
三個(gè)沒滅菌和六個(gè)滅菌膠原蛋白涂層薄培養(yǎng)載片 （載片規(guī)格：75 mm x 24 mm x 0.2 mm）
三個(gè)沒滅菌和六個(gè)滅菌膠原涂層StageFlexer膜
配件包
保證細(xì)胞在不同水平恒流或生理剪切力作用下仍保持黏附，在研究中得到了廣泛應(yīng)用。用蠕動(dòng)泵（peristaltic pump）或注射泵（syringe pump）提供瞬態(tài)剪切力使平行板流室的入流管和出流管之間產(chǎn)生壓差，使流室內(nèi)細(xì)胞受到均勻，震蕩或脈動(dòng)剪切力的作用
微流納流HiQ Flowmate微流體控制器
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