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          世聯(lián)博研(北京)科技有限公司>供求商機(jī)>orflo代理MOXI Z迷你細(xì)胞計(jì)數(shù)器 moxi flow迷你流式細(xì)胞儀,微型自動(dòng)細(xì)胞計(jì)數(shù)儀
          orflo代理MOXI Z迷你細(xì)胞計(jì)數(shù)器 moxi flow迷你流式細(xì)胞儀,微型自動(dòng)細(xì)胞計(jì)數(shù)儀
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          貨物所在地: 北京北京市
          更新時(shí)間: 2024-09-21 21:00:07
          期: 2024年9月21日--2025年3月21日
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          產(chǎn)品簡(jiǎn)介

          Orflo中品牌的Moxi Flow微型流式細(xì)胞儀及Moxi Z細(xì)胞計(jì)數(shù)儀,Moxi Z 微型自動(dòng)細(xì)胞計(jì)數(shù)儀配套芯片(Cell Counting Cassettes),Moxi Z S型細(xì)胞計(jì)數(shù)芯片(Moxi Z Type S Cassettes),Moxi Z M型細(xì)胞計(jì)數(shù)芯片(Moxi Z Type M Cassettes)

          詳細(xì)介紹

          簡(jiǎn)單介紹

          世聯(lián)博研(北京)科技有限公司正式做為Orflo中國(guó)代理商,主要產(chǎn)品包括Moxi Flow微型流式細(xì)胞儀及MoxiZ細(xì)胞計(jì)數(shù)產(chǎn)品系列,迷你細(xì)胞計(jì)數(shù)儀Moxi Z和掌上流式細(xì)胞儀MOXI FLOW。 Moxi GO & Moxi GO II orflo,mxf002,Moxi Flow Kit, US Version

          產(chǎn)品描述

                                 備有庫(kù)存現(xiàn)貨,隨時(shí)發(fā)貨

           

          ORFLO Technologies總部位于美國(guó)愛(ài)達(dá)荷州,擁有多項(xiàng)技術(shù),如微流控、生物信號(hào)向電子信號(hào)轉(zhuǎn)化、數(shù)據(jù)采集和分析、配套分析軟件設(shè)計(jì)等,致力于現(xiàn)代細(xì)胞分析技術(shù)創(chuàng)新和開(kāi)發(fā),在細(xì)胞分析技術(shù)競(jìng)爭(zhēng)中處于的地位。

          ORFLOTechnologies 2010年開(kāi)發(fā)*個(gè)掌上細(xì)胞計(jì)數(shù)儀,產(chǎn)品一經(jīng)推出,很快受到業(yè)內(nèi)廣泛認(rèn)可。目前推出代迷你細(xì)胞計(jì)數(shù)儀Moxi Z和掌上流式細(xì)胞儀MOXI FLOW。

           

          世聯(lián)博研(北京)科技有限公司正式做為Orflo中國(guó)代理商,主要產(chǎn)品包括Moxi Flow微型流式細(xì)胞儀及MoxiZ細(xì)胞計(jì)數(shù)產(chǎn)品系列等。

           

          美國(guó)ORFLO公司推出的一臺(tái)手持式微型流式細(xì)胞儀。她的外觀靈巧,*可將她托放與掌中,便于移動(dòng),讓你的實(shí)驗(yàn)地點(diǎn)不再被龐大的儀器所束縛;操作便捷,無(wú)需受過(guò)專業(yè)培訓(xùn)就可以完成操作,因?yàn)樗胁僮骶椭挥袃刹剑≒lug&Play),上機(jī)10秒后就可在彩色觸屏顯示器上看到實(shí)驗(yàn)結(jié)果,簡(jiǎn)便*。價(jià)錢(qián)方面,對(duì)于絕大多數(shù)的實(shí)驗(yàn)室來(lái)說(shuō),如果想擁有她,從經(jīng)濟(jì)角度上講是*可行的??吹竭@里估計(jì)讀者已經(jīng)迫不及待的想進(jìn)一步了解和認(rèn)識(shí)她了,下面就由筆者向各位介紹這位靈動(dòng)、聰慧的姑娘 — MoxiFlow(圖1)。

           

          內(nèi) 外 兼 修

          對(duì)于它外觀的小巧此處不再累述(大小規(guī)格僅為26×15×15cm),圖片比文字更有說(shuō)服力(圖2);儀器內(nèi)置4500mAh鋰電池,便于在實(shí)驗(yàn)室內(nèi)的移動(dòng)和隨意放置;內(nèi)置軟件程序,480X320pix彩色顯示器可清晰呈現(xiàn)數(shù)據(jù)結(jié)果,讓您一目了然。至于檢測(cè)方式,它采用了兩種檢測(cè)的*融合—庫(kù)爾特電阻原理與熒光檢測(cè)原理:

          圖2 手持式流式細(xì)胞儀

          一、 庫(kù)爾特原理(用于檢測(cè)細(xì)胞大小及數(shù)量)
          當(dāng)懸浮于液體中的細(xì)胞通過(guò)小孔管時(shí),會(huì)取代相同體積的液體,在恒定電流設(shè)計(jì)的電路中導(dǎo)致小孔管內(nèi)外的電阻發(fā)生瞬時(shí)變化,產(chǎn)生電位脈沖,而這種脈沖信號(hào)的大小和次數(shù)與細(xì)胞的大小與數(shù)目成正比,因此可用于細(xì)胞計(jì)數(shù)及大小的檢測(cè)。這正是談到流式細(xì)胞儀的歷史而不可避開(kāi)的庫(kù)爾特原理,庫(kù)爾特電阻檢測(cè)法屬于對(duì)細(xì)胞個(gè)體的測(cè)量和三維的測(cè)量,不但能準(zhǔn)確測(cè)量細(xì)胞的大小,更能對(duì)細(xì)胞的數(shù)量及濃度進(jìn)行測(cè)量。利用庫(kù)爾特原理檢測(cè)細(xì)胞大小更近真實(shí),而不像激光衍射散射原理會(huì)受到染料顏色和濃度的影響。流式細(xì)胞儀(FCM,Flow cytometry),從字面上的cytometry (細(xì)胞計(jì)數(shù)) 也看得出它與細(xì)胞計(jì)數(shù)有著一定的淵源,Wallace Coulter于1947年發(fā)明了庫(kù)爾特原理,用于血細(xì)胞分析,隨后1953年Coulter公司便推出了世界上*臺(tái)流式細(xì)胞分析儀。而以庫(kù)爾特原理為主導(dǎo)技術(shù)開(kāi)發(fā)細(xì)胞計(jì)數(shù)儀的ORFLO公司在此推出這樣一臺(tái)眾望所歸的微型流式細(xì)胞儀也便是名正言順了,并利用其的薄膜傳感器技術(shù)對(duì)細(xì)胞進(jìn)行數(shù)量、直徑等檢測(cè),獲取更加精準(zhǔn)可靠的數(shù)據(jù)。

          二、 熒光檢測(cè)(用于檢測(cè)熒光信號(hào))
          MoxiFlow配置了一根532nm固態(tài)激光器和一個(gè)PMT,檢測(cè)光譜區(qū)域?yàn)?90/40nm ( 可檢測(cè)染料:R-PE, PI, Nile Red, Ethidium Homodimer I & III, Sytox Orange等 )。對(duì)細(xì)胞大小檢測(cè)的同時(shí)以傳統(tǒng)流式的方法利用激光激發(fā)標(biāo)記在細(xì)胞上的特異性熒光染料,并通過(guò)PMT進(jìn)行檢測(cè),獲取熒光信號(hào)。

          MoxiFlow 即利用了以上兩種檢測(cè)方式相結(jié)合的方式,在細(xì)胞流經(jīng)小孔管時(shí),同時(shí)對(duì)細(xì)胞的大小、數(shù)目及熒光信號(hào)進(jìn)行的檢測(cè)(如圖3)。

                                

           

          化 繁 為 簡(jiǎn)

          一、 簡(jiǎn)易化操作
          微流體系膜片內(nèi)設(shè)細(xì)胞過(guò)濾功能,所以對(duì)于準(zhǔn)備上機(jī)的細(xì)胞懸液無(wú)需進(jìn)行濾網(wǎng)的過(guò)濾,直接即可加入膜片孔內(nèi)準(zhǔn)備實(shí)驗(yàn)(如圖4),此外膜片的窗口處為非透明材質(zhì)做成,形成小的避光暗室,避免了熒光檢測(cè)過(guò)程中熒光猝滅現(xiàn)象;僅需兩步即可完成所有操作步驟。一,將細(xì)胞微流膜片插入檢測(cè)口;二,將50μL樣品加入檢測(cè)孔內(nèi),點(diǎn)擊Play即可開(kāi)始檢測(cè) (如圖5~6);僅10秒鐘即可顯示出實(shí)驗(yàn)結(jié)果。輸出數(shù)據(jù)的格式為標(biāo)準(zhǔn)的流式數(shù)據(jù)格式FCS3.1或.BMP的圖像輸出格式,結(jié)果數(shù)據(jù)可以以散點(diǎn)圖及直方圖(如圖7)的形式表現(xiàn)出來(lái),并可輕松用手觸摸。

          二、 程序化實(shí)驗(yàn)
          MoxiFlow將較為常用的流式實(shí)驗(yàn)程序化為軟件中的多個(gè)選項(xiàng) (如圖8,并在不斷更新升級(jí)中),在實(shí)驗(yàn)室過(guò)程中只需點(diǎn)擊要進(jìn)行的實(shí)驗(yàn)名稱即可完成相應(yīng)的數(shù)據(jù)檢測(cè),如: 細(xì)胞活性檢測(cè)、 細(xì)胞凋亡檢、 多參數(shù)分析、細(xì)胞大小檢測(cè)、熒光珠子檢測(cè)、 細(xì)胞周期檢測(cè)(coming soon)等,使用系統(tǒng)內(nèi)相應(yīng)實(shí)驗(yàn)程序即可,應(yīng)用哪里,點(diǎn)哪里,無(wú)需再對(duì)電壓、熒光補(bǔ)償?shù)葏?shù)進(jìn)行調(diào)整。沒(méi)有經(jīng)過(guò)任何專業(yè)培訓(xùn)的人員也可輕松操作完成,可見(jiàn)MoxiFlow解放了科研人員對(duì)于流式細(xì)胞儀使用操作的繁瑣性及專業(yè)性,是對(duì)流式細(xì)胞儀的一次嶄新的革命。

             現(xiàn)

          為了展現(xiàn)出MoxiFlow的檢測(cè)性能,下面分別將MoxiFlow與臺(tái)式流式細(xì)胞儀、成像細(xì)胞分析系統(tǒng)、血細(xì)胞計(jì)數(shù)板進(jìn)行比較。結(jié)果發(fā)現(xiàn),對(duì)于細(xì)胞的計(jì)數(shù)誤差要低于其他類型產(chǎn)品,而計(jì)數(shù)平均值的變異系數(shù)(CV)也是zui小的,可見(jiàn) MoxiFlow對(duì)于細(xì)胞數(shù)量的檢測(cè)表現(xiàn)(圖9 左);對(duì)于細(xì)胞活性的檢測(cè)誤差值與平均活性檢測(cè)的波動(dòng)性(CV)都是zui小的,即在對(duì)細(xì)胞活性檢測(cè)的實(shí)驗(yàn)中,MoxiFlow依然很好地保證了數(shù)據(jù)的精準(zhǔn)度和穩(wěn)定性(圖9 右)。

          下圖是MoxiFlow細(xì)胞凋亡實(shí)驗(yàn)的結(jié)果圖,此實(shí)驗(yàn)是用喜樹(shù)堿(一種抗癌藥物)對(duì)Jurkat 細(xì)胞(白血病細(xì)胞)分別刺激不同時(shí)間(2h,6h,7h)所得到的散點(diǎn)圖??梢栽u(píng)測(cè)出該藥物對(duì)Jurkat細(xì)胞凋亡的作用,并給出各個(gè)群落的細(xì)胞數(shù)量、細(xì)胞濃度、占細(xì)胞總數(shù)的百分比及細(xì)胞的平均直徑等信息。

           

           

          總結(jié):

          外形的小巧并沒(méi)有影響MoxiFlow的應(yīng)用性能,所謂,麻雀雖小五臟俱全。它可幫你擺脫對(duì)流式細(xì)胞儀“想愛(ài)不敢愛(ài)”的矛盾心理,讓流式不再高不可攀,讓細(xì)胞在你的掌中流動(dòng)。

           

           

           

          英文介紹:

           

           

          Flow Cytometers

           

          ORFLO delivers simple, affordable, yet powerful solutions for flow cytometry and cell analysis. Now you can focus your efforts on research and driving your pace of discovery, rather than all the complexity that comes with traditional, complicated flow cytometers. 

          ORFLO has revolutionized flow cytometry by integrating traditional fluorescence based flow cytometry with the Coulter Principle. Now for the first time direct cell counts and volume are generated in parallel with fluorescence. At the heart of ORFLO's platforms is their disposable flow cytometer sensor, which eliminates the need for cleaning, maintenance, expensive service contracts and calibration.

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Moxi Flow

          World's most affordable flow cytometer, ideal for single color flow, cell health and cell QC.

           

          Learn More

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Moxi GO & Moxi GO II

          Increase the pace of scientific discovery with ORFLO’s next generation Moxi GO™ & Moxi Go II™ Next Gen Flow Cytometers.

           

          Learn More

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

           

           

           

           

           

           

           

           

           


          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXF002

           

          Moxi Flow instrument with USB power cord, US style USB power adapter, and Type MF-S cassette pack

           

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           


          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Supporting Resources

           

          Application Notes

          Brochures

          Protocols

          Quick Start Guides

          Software Downloads

          Technical Notes

          User Manuals

          Customer Data

          Product Videos

          Related Products

           

          Reagents

          Assays

          Accessories

          Antibodies

          Cassettes

          Instruments

           
          • Overview 
          •   
            • Specifications 
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              • How It Works 
              •   < style="padding:0px;overflow:visible !important;display:inline;" >Customer Data
                 

          “Plug and Play” Simplicity - Moxi Flow is the World’s first SMART FLOW CYTOMETER.

           

           

          “Plug and Play” Simplicity

           

           

          Moxi Flow is a fully automated, cassette-based flow cytometer that combines unparalleled ease of use with the precision and accuracy normally only associated with more expensive flow cytometers. This ultra-small instrument uses patented microfluidic thin-film cassettes that enable automatic load and measure operation. The Moxi Flow is also the only flow cytometer that auto-aligns the laser to each cassette to enable highly repeatable and robust results every time. No PMT gain adjustment is required. No warm up time required. Just insert a cassette, pipette your sample, and read the results in 10 seconds.

          Versatile

           

           

          Select from one of our standard applications (i.e., Cell Counts, Viability, Apoptosis) or run the system in open “2 Parameter Flow Cytometry” mode for the ultimate in assay flexibility. This miniature open platform can be used for your most common cell assay needs, or to develop sophisticated, custom assays right at your fingertips.

          Access your FCS compatible Data with “USB-on-the-Go”

           

           

          Moxi Flow generates data using the industry standard FCS 3.1 format so you can open and analyze your results using your ORFLO’s VESTIGO software (coming soon), or any other standardized flow cytometry analysis software. Data is transferred simply and easily to a PC or MAC using a mini-USB cable and the on board “USB-on-the-Go” functionality. No additional software is required to perform the data transfer.

          • Cell Count with >95% accuracy
          • Viability with PI
          • Apoptosis with Annexin V
          • Precise cell/particle (Coulter Principle)
          • Blood analysis (RBC counts, WBC counts, CD4 counts, etc.)
          • Somatic Cell Counts in Milk


           

          Cell Count and Viability

           

           

          Flow cytometry based viability tests with simultaneous Coulter Principle Cell Counts and Cell Volume

           

           

          Below are screen shots taken directly from the Moxi Flow. The left screen shot is a scatter plot of fluorescence intensity of the Propidium Iodide (PI), used to select for dead cells, versus cell volume on the x axis, converted to cell diameter. The middle screen shot is cell volume only, which shows the cell volume heterogeneity of the cancer line population along with subtle shift in the mean cell volume of the dead cell population. The right screen shot shows the fluorescence intensity of the baseline viable cell population versus the dead cell population.

           

          Propidium Iodide was used to detect the exact cell count of the dead, non viable cells. The viability percent is displayed in the yellow text box. The concentration and volume of the viable live cells and non viable cells is displayed in the black text boxes.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          Apoptosis (Annexin V) and Cell Count

           

           

          Below are screen shots taken directly from the Moxi Flow. The left screen shot is a scatter plot of fluorescence intensity of the Annexin V PE versus cell volume on the x axis, converted to cell diameter. The right screen shot is cell volume only, which shows the cell volume heterogeneity of the cancer line population. Annexin V conjugated with PE was used to detect the exact number of cancer cells that were apoptotic. The percent of apoptotic cells is displayed in the yellow text box. The concentration and volume of the non-apoptotic and apoptotic cells is displayed in the black text boxes. This together with the above cell viability and cell count tests gives a read of over all cell health.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          Cell Count and Transfection Efficiency Checks

           

           

          Transfection Efficiency Flow Cytometery

           

           

          Below are screen shots taken directly from the Moxi Flow. The left screen shot is a scatter plot of fluorescence intensity of the RFP (tdTomato, DS Red, Nile Red, etc.) versus cell volume on the x axis, converted to cell diameter. The right screen shot is fluorescence only, which shows heterogeneity the transfect RFP within the cancer line population. The RFP in this case was tdTomato. In this example there were two distinct populations of the transfected cell lines. Both are * positively transfected. It is possible that this could be an inidication of cell cycle, the lower population could be in G0/G1 phase with approximay half the DNA of the upper population, G2/M phase.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          Rapid Immuno Profiling of White Blood Cells, PBMC’s, Whole Blood

           

           

          Immono Profiling Flow Cytometry

           

           

          In all tests shown in the figures below Tonbo PE conjugated flow cytometery antibodies were used.

           

          In the screen shots below the PBMC sample was screened for the number of CD2 positive versus CD2 negative cells, using Tonbo’s PE Anti Human CD2 antibody (RPA-2.10).

           

          The RPA-2.10 antibody reacts with human CD2, an approximay 50 kDa glycoprotein, and a member of the Ig superfamily. CD2, also known as LFA-2, is a receptor for CD58 in the human and is expressed on the cell surface of 80-90% of human peripheral blood lymphocytes, a subset of NK cells, and all mature T cells. CD2 mediates lymphocyte adhesion and is involved in T cell activation. RPA-2.10 is reported to block mixed lymphocyte reaction. Please choose the appropriate format for each application.

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD2 positive cells versus the CD2 negative cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          In the screen shots below the PBMC sample was screened for the number of CD3 positive versus CD3 negative cells, using Tonbo’s PE Anti Human CD3 antibody (Hit3a).

           

          The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells.

           

          The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3. Please choose the appropriate format for each application.

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD3 positive white blood cells versus the CD3 negative white blood cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.



           
           

          In the screen shots below the PBMC sample was screened for the number of CD4 positive (t cells) versus CD4 negative cells, using Tonbo’s PE Anti Human CD4 antibody (OKT4).

           

          The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The OKT4 antibody is widely used as a phenotypic marker for CD4 expression. It is cross-reactive with CD4 in several non-human species, including Chimpanzee, Cynomolgus and Rhesus. This antibody recognizes a different epitope, and thus does not block binding of, the alternative Anti-Human CD4 antibody clone RPA-T4 (Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061-4065)

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD4 positive white blood cells versus the CD4 negative white blood cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          In the screen shots below the PBMC sample was screened for the number of CD8 positive versus CD4 negative cells, using Tonbo’s PE Anti Human CD8 antibody (SK1).

           

          The SK1 antibody is specific for the 32-34 kDa alpha chain of human CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.

           

          The SK1 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells. It is cross-reactive with CD8 in several non-human species, including Baboon, Chimpanzee, Cynomolgus and Rhesus. If used together with an alternative Anti-Human CD8a clone, RPA-T8, the SK1 antibody will not block binding of RPA-T8 to CD8a.

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD8 positive white blood cells versus the CD8 negative white blood cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          In the screen shots below the PBMC sample was screened for the number of CD11 positive versus CD11 negative cells, using Tonbo’s PE Anti Human CD8 antibody (ICRF44).

           

          The ICRF44 antibody reacts with human CD11b, also known as integrin alpha M. This 165-170 kDa cell surface glycoprotein is part of a family of integrin receptors that mediate adhesion between cells (cell-cell) and components of the extracellular matrix, e.g. fibrinogen (cell-matrix). In addition, integrins are active signaling receptors which recruit leukocytes to inflammatory sites and promote cell activation. Complete, functional integrin receptors consist of distinct combinations of integrin chains which are differentially expressed. Integrin alpha M (CD11b) assembles with Integrin beta-2 (CD18) into a receptor known as Macrophage Antigen-1 (Mac-1) or complement receptor type 3 (CR3). This receptor binds and induces intracellular signaling through ICAM-1, ICAM-2, ICAM-3 and ICAM-4 on endothelial cells and can also facilitate removal of iC3b bearing foreign cells.

           

          The ICRF44 antibody is widely used as a marker for CD11b expression on macrophages, granulocytes, and subsets of NK cells. It is reported to be cross-reactive with a number of non-human species including Baboon, Chimpanzee, Cynomolgus, Rhesus and Swine.

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD11 positive white blood cells versus the CD11 negative white blood cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.



          orflo品牌MOXI Z Mini Automated Cell Counter Kit 
           

          In the screen shots below the PBMC sample was screened for the number of CD19 positive b cells versus CD19 negative white blood cells, using Tonbo’s PE Anti Human CD19 antibody (SJ25C1).

           

          The SJ25C1 antibody reacts with human CD19, a 95 kDa glycoprotein which acts as a co-receptor, along with CD21 (CR2), CD81 (TAPA-1) and CD225 (Leu13), in support of the functional B cell receptor (BCR). This complex provides antigen-specific recognition and subsequent activation of B cells to proliferate and differentiate into antibody-secreting cells (plasma cells) or memory B cells, which are crucial for secondary antigen encounter. Upon activation and tyrosine phosphorylation, the CD19 molecule can provide an anchor for cytoplasmic signaling proteins such as GRB2, SOS or PLCG2. CD19 is a lineage-differentiation marker, as its expression is detectable at the earliest B cell stages, through development, and is finally lost upon transition to mature plasma cells.

           

          The SJ25C1 antibody is widely used as a phenotypic marker for CD19 expression on B cells, as well as on dendritic cell subsets.

           

          The Moxi Flow Flow Cytometer Cell Counter (Coulter Principle based) is able to clearly separate out the CD19 positive white blood b cells versus the CD19 negative white blood cells, delivering a highly accurate and precise count of the two populations. The fold difference between the positive and negative white blood cells is a simple relative protein quantitation method.


          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          Moxi GO & Moxi GO II
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXG001_MXG002_MXG102

           

          Moxi GO 532nm laser (MXG001)
          1 PMT - 561nm/LP filter (e.g. PE, PI) - US Version
          Moxi GO 488nm laser (MXG002)
          1 PMT - User-swappable 525/45nm (e.g. FITC, GFP) or 561nm/LP (PE,PI) filters
          Moxi GO II 488nm laser (MXG102)
          2 PMT system 525/45nm (e.g. FITC, GFP) and 561nm/LP (PE, PI)

           

          See Product Specifications

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

           

           

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          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           


          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Supporting Resources

           

          Application Notes

          Brochures

          Protocols

          Quick Start Guides

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          • Overview 
          •   
            • Specifications 
            •   < style="padding:0px;overflow:visible !important;display:inline;" >How It Works
               

          3 or 4 Parameter Next Gen Flow

           

           

          Increase the pace of scientific discovery with ORFLO’s next generation Moxi Go™ or Moxi Go II™ Next Gen Flow Cytometers. With a one (Moxi GO) or two (Moxi GO II) fluorescent channels, swappable filter sets (MXG002 - Moxi GO 488nm), configurable laser the Moxi Go can be designed to meet your labs cell analysis needs.  Every assay also delivers simultaneous cell count & volume determination, using single-use flow cell.  This eliminates the hassle of traditional flow associated with cleaning, maintenance, clearing of clogs, cross contamination and occasionally replacement of bottles and tubes.  

           

          Furthermore the Moxi Go™ or Moxi Go II™ use very little sample volume, 75ul's allowing you to conserver precious, expensive sample, such as stem cells.  Cell concentrations as low as 10,000 cells per ml are possible, which most experiments would enable a little as 5ul's of sample diluted in 70ul's of PBS.

           

          The Moxi Go™ or Moxi Go II™ can be utilized as an assay development instrument and many powerful cell based assays can easily be optimized and run on the system.  These include:

          • Cell surface immuno-labeling
          • In-Cell Protein Quant
          • CRISPR/Transfection Optimization Studies
          • Up to 4 plex bead based ELISA's using our new on board MPX Relative Quantitation Ap
          • In cell Wester Blots
          • Reactive Oxidation Species Experiments
          • Calein AM studies
          • Phagocytosis Analysis
          • Mito Potential Experiements
           

          The Moxi Go™ or Moxi Go II™ come standard with an ultra-intuitive, plug-and-play interface with free OS updates as long as you own the instrument. No prior flow cytometry experience is required you simply just plug and play.

           

           

           

           

           

           

          配套耗材:

           

          一、Moxi Z 微型自動(dòng)細(xì)胞計(jì)數(shù)儀配套芯片(Cell Counting Cassettes)

          芯片覆蓋直徑范圍(2-34微米)內(nèi)的所有細(xì)胞樣品,無(wú)需根據(jù)細(xì)胞大小進(jìn)行更換,

          每個(gè)芯片可做2個(gè)測(cè)試。當(dāng)樣品1完成后,只需取出,將芯片的另一端插入Moxi Z,加入樣品2。

          在一次性盒式芯片中具有更高的性能和簡(jiǎn)單性。

          Moxi Z*的保護(hù)薄膜細(xì)胞計(jì)數(shù)盒可在8秒鐘內(nèi)提供高精度,可重復(fù)的細(xì)胞計(jì)數(shù)和細(xì)胞大小分析(S型盒帶15秒)。受計(jì)數(shù)系統(tǒng)使用的金標(biāo)準(zhǔn)Coulter原理的啟發(fā),Moxi盒每個(gè)測(cè)試通過(guò)一個(gè)細(xì)胞感測(cè)區(qū)域流動(dòng)數(shù)千個(gè)細(xì)胞,以準(zhǔn)確捕獲樣品中每個(gè)單個(gè)細(xì)胞的基于阻抗的體積測(cè)量?;趫D像的細(xì)胞計(jì)數(shù)方法,單個(gè)細(xì)胞的基于阻抗的細(xì)胞計(jì)數(shù)被證明是超過(guò)95%的準(zhǔn)確度,而75%的準(zhǔn)確度。

          沒(méi)有系統(tǒng)污染和集成預(yù)過(guò)濾器防止堵塞。

          Moxi盒式芯片設(shè)計(jì)有*的“細(xì)胞篩”,以zui大限度地減少細(xì)胞結(jié)塊和堵塞。另外,Moxi盒含有100%的樣品在盒體內(nèi),消除了系統(tǒng)污染和滅菌的可能性。

          1、Moxi Z S型細(xì)胞計(jì)數(shù)芯片(Moxi Z Type S Cassettes)

           

          The Type S cassette is ideal for accuray measuring most yeast and other particles as small as 3 µm and up to 20 µm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accuray.

          S型細(xì)胞計(jì)數(shù)芯片非常適用于測(cè)量大多數(shù)酵母和其他顆粒,平均粒徑小至3μm,直徑達(dá)20μm,包括一些藻類和原生動(dòng)物。 由于該技術(shù)基于體積測(cè)量,因此也可以準(zhǔn)確測(cè)量非球形顆粒(14-4,200fL)。

           

           

          Cell Counting Cassettes

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Higher Performance & Simplicity in One Disposable Cassette.
          Moxi Z's unique, patent-protected thin-film cell count cassettes provide highly accurate, repeatable cell counts and cell size analysis in under 8 seconds (15 seconds for Type S cassette). Inspired by the gold-standard Coulter Principle used in high-end counting systems worldwide, the Moxi cassette flows thousands of cells per test through a cell sensing zone to accuray capture the impedance-based volumetric measurement of each individual cell in the sample. Impedance-based cell counting of individual cells is proven to be over 95% accurate versus a 75% accuracy with image-based cell counting methods. 

          No system contamination and integrated pre-filter for clogging prevention. 
          Moxi cassettes are designed with a unique "cell sieve" to minimize cell clumping and clogging. In addition, Moxi cassettes contain * of your sample within the cassette body, eliminating the possibility for system contamination and sterilization. Cassettes are simply and easily discarded when testing is finished.

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Moxi Z Type S Cassettes

          The Type S cassette is ideal for accuray measuring most yeast and other particles as small as 3 µm and up to 20 µm in average diameter including some algae and protozoa. Since the technology is based on a volumetric measurement, non-spherical particles (14 - 4,200 fL) can also be measured accuray.

           

          Moxi Z Type S Pack (25 Cassettes)

            |  

          Moxi Z Type S Box (250 Cassettes)

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

           

          Moxi Z Type M Cassettes

          The Type M cassette is ideal for accuray measuring mammalian cells or other particles 4-25 microns in average diameter. Since the technology is based on a volumetric measurement, non-spherical particles (34-8,180 fL) can also be measured accuray.

           

          Moxi Z Type M Pack (25 Cassettes)

            |  

          Moxi Z Type M Box (250 Cassettes)

           

           

           

           

           

           

          MOXI Z System Check Beads (5 mL)
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXA005

          MOXI Z System Check Beads (5 mL)

           

          orflo品牌MOXI Z Mini Automated Cell Counter KitSh List

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Related Products

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          • Specifications
           
          idMXA005
          Application NotesCan be used to check proper system function.
          Bead Diameter10 micron
          Compatible InstrumentsMoxi Z
          Concentration1.1 e+5 beads/mL
          FormulationOrflo Diluent
          Intended Use StatementFor Research Use Only. Product is not for use in diagnostic procedures
          Material of ConstructionPolystyrene
          Overall Dimensions22.1 mm^3
          SterilityNon-sterile
          Weight5 mg

           

           

          Moxi Z Diluent
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXA006

          Moxi Z Diluent, 100 mL

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Related Products

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          • Specifications
           
          idMXA006
          Application NotesMoxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
          ApplicationsCell Dilution
          Applications AbbreviationCdil
          Cassette TypesType M|Type S
          Compatible InstrumentsMoxi Z
          FormulationProprietary
          Intended Use StatementFor Research Use Only. Product is not for use in diagnostic procedures
          Kit ComponentsOne 100 ml bottle of diluent
          Number of Testsn/a
          Overall Dimensions52 L x 52 W x 106 H (mm)
          ProtocolPellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
          Sample TypeMammalian Cells|Large Yeast|Large Algae|Protozoa
          SterilityNon-Sterile
          Storage Stability AND HandlingStore at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
          VendorORFLO
          Weight152 g

           

           

           

          Moxi Z Diluent, sample
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXA009

          Moxi Z Diluent,  5 mL

          See Product Specifications

          orflo品牌MOXI Z Mini Automated Cell Counter Kit

           

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Related Products

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          Accessories

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          • Specifications
           
          idMXA009
          Application NotesMoxi Z Diluent is special formulated for use with the Moxi Z system for optimal sizing of particles and for operation with the instrument's Small Particle Mode (SPM).
          ApplicationsCell Dilution
          Applications AbbreviationCdil
          Cassette TypesType M|Type S
          Compatible InstrumentsMoxi Z
          FormulationProprietary
          Intended Use StatementFor Research Use Only. Product is not for use in diagnostic procedures
          Kit ComponentsOne 5 ml bottle of diluent
          Number of Tests75
          Overall Dimensions25 W x 45 H (mm)
          ProtocolPellet and resuspend cells in Moxi Z diluent or dilute sample with >90% Moxi Z diluent. 2. Pipette 10 uL of sample into tube 3. Pipette 90 uL of Orflo Diluent into tube 4. Gently mix resulting solution to ensure cells are evenly mixed 5. Pipette 75 uL of resulting solution into Moxi Z Cassette, or 50 uL into Moxi Flow cassette 6. Touch screen to run test
          Sample TypeMammalian Cells|Large Yeast|Large Algae|Protozoa
          SterilityNon-Sterile
          Storage Stability AND HandlingStore at 2-8C. Use sterile technique when handling. Dispose of waste product in compliance with federal, state and local regulations. Do not inhale or ingest. Avoid contact with eyes and skin, flush affected area with water for at least 15 minutes
          VendorORFLO
          Weight11 g
          Orflo Accutaseª
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXA020

          Orflo Accutase cell detachment solution, 1X, 100 mL

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Supporting Resources

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          • Specifications
           
          idMXA020
          Application NotesRecommended for the detachment of adherent cells and dissociation of mammalilian cell lines and primary cells
          ApplicationsCell Detachment
          Applications AbbreviationCdet
          Cassette TypesType M|Type S|Type MF-M|Type MF-S
          Cell Types Testedfibroblasts|keratinocytes|vascular endothelial cells|hepatocytes|vascular smooth muscle cells|hepatocyte progenitors|primary chick embryo neuronal cells|bone marrow stem cells|adherent CHO and HNK cells|macrophages|293 cells|L929 cells|immortalized mouse testicular germ cells|MIC5|3T3|Vero|COS|HeLa|NT2|MG63|M24 and A375 metastatic melanoma|gliomas U251|D54|HT1080 fibrosarcoma cells|HT29 colon cancer cells|Sf9 insect cells|human embryonic stem cells|human mesenchymal stem cells|and human neural stem cells.
          Compatible InstrumentsMoxI Z|Moxi Flow|zEPI Flow
          FormulationAccutase® enzymes in Dulbecco's PBS containing 0.5 mM EDTA and phenol red. 1X concentrate, no preservatives.
          Intended Use StatementFor Research Use Only. Product is not for use in diagnostic procedures
          Kit ComponentsOne 100ml bottle of cell dissassociation reagent
          Number of Testsn/a
          Overall Dimensions52 L x 52 W x 106 H (mm)
          Protocol1. Place 100ml Accutase bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accutase per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accutase c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
          Sample TypePrimary Mammalian cells|immortalized adherent cells|non-ES cell types
          SterilitySterile
          Storage Stability AND HandlingStable at -20C. After thawing can be stored for up to 2 months at 2-8C.
          TradenameACCUTASE™ is a registered trademark of Innovative Cell Technologies
          VendorORFLO
          Weight159 g

           

           

           

          Orflo Accumaxª
          orflo品牌MOXI Z Mini Automated Cell Counter Kit

          Product Code: MXA021

          Orflo Accumax, cell dissassociation reagent, 10X, 100 mL, sterile

           

           

          orflo品牌MOXI Z Mini Automated Cell Counter Kit



           
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
           
            
           
           
          orflo品牌MOXI Z Mini Automated Cell Counter Kit
          orflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kitorflo品牌MOXI Z Mini Automated Cell Counter Kit

          Supporting Resources

          Related Products

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          • Specifications
           
          idMXA021
          Application NotesRecommended for use with the Moxi Z and Moxi Flow platforms when working with clumpy aggregated cells. It is ideal for generating single cell suspensions from clumped cell cultures, detachment of cells from primary tissue and detachment of cells from plastic tissue culturware. Accumax is specifically designed for our combination fluorescence amp impedance based flow cytometers.
          ApplicationsCell Detachment
          Applications AbbreviationCdet
          Cassette TypesType M|Type S|Type MF-M|Type MF-S
          Compatible InstrumentsMoxI Z|Moxi Flow|zEPI Flow
          Formulation10X proprietary solution of proteolytic, collagenolytic and DNase enzymes and does not contain mammalian or bacterial derived products.
          Intended Use StatementFor Research Use Only. Product is not for use in diagnostic procedures
          Kit ComponentsOne 100ml bottle of cell dissassociation reagent
          Number of Testsn/a
          Overall Dimensions52 L x 52 W x 106 H (mm)
          Protocol1. Place 100ml Accumax bottle in room temp water bath or thaw overnight in fridge 2. For Adherent Cells: a. Remove cell media b. Add 1.5ml Accumax per 25cm2 of flask area c. incubate at RT or 37C until cells detach (typically 5-15min) d. Pipette triturate to break apart clusters 3. For suspension cell aggregates: a. Pellet cells at 300xg, 5min b. Resuspend pellet in 1-2ml Accumax c. Incubate 15min d. Pipette triturate to break apart clusters 4. Count cells on the Moxi Z, Moxi Flow, or Zepi Flow (be sure to dilute samples into standard operating range of specific cassette, if they are above the stated concentration limits)
          Sample TypePrimary Mammalian cells|immortalized adherent cells|Human ES cells|Rodent ES
          SterilitySterile
          Storage Stability AND HandlingStable at -20C. After thawing can be stored for up to 2 months at 2-8C.
          TradenameACCUMAX™ is a registered trademark of Innovative Cell Technologies
          VendorORFLO
          Weight159 g

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