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針式可編程組合微電極
The microelectrode is ready to use. Carefully unwind the tape and remove the probe
from the protective glass tube. The white translucent sleeve, which covers the hole,
should be moved down

針式可編程組合微電極

Calibration
The electrode can be held by hand when measuring pH. It has been standardized in
two (2) pH buffers. Follow the procedure recommended by the manufacturer of your pH
meter for calibration

針式可編程組合微電極

Optimum Response Time: Optimum response time will be obtained after the probe
has been exercised in two (2) buffer solutions. Fill one beaker with a pH 4.01 buffer orequivalent and a second beaker with a pH 6.86 buffer or equivalent. Hold themicroelectrode and touch the pH 4 .01 buffer surface allowing 5 seconds for
equilibration, and then touch the pH 6.86 buffer surface in the same0 manner. Do thisseveral times. It is not necessary to rinse the probe with distilled water because thecarry-over is negligible

針式可編程組合微電極

Handling
The outer reference chamber of the microelectrode can be refilled when necessary withthe 3 M KCl solution, using the bulb pipette. Hold the electrode at a 60 degree angle(approx.) and slowly insert the tip into the fill hole of the electrode.
Be careful not to apply pressure against the inner glass capillary tube.
Slowly fill the reference chamber. If filling is done too quickly, air may be trapped in the chamber causing reference solution to squirt out of the fill hole instead of entering and filling the reference chamber

Cleaning
When using the microelectrode in solutions containing protein, it should be soaked in an
enzyme cleaning solution such as Terg-a-zyme (Alconox, Inc.) or a chromic/sulfuric
acid glass cleaning solution for a couple of minutes after each use to remove the protein
from the glass and the reference junction. This will prolong the useful life of the
microelectrode.
Storage
Always clean the microelectrode before storing:
Long-term (over 2 weeks): Return the electrode to its original container and prepare it
in the same condition in which you received it. Usually this means simply moistening
the sponge located in the bottom of the protective glass tube with pH 4 buffer.
Short-term: The probe can be left in an acid pH buffer solution (pH 4.01)