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世聯(lián)博研（北京）科技有限公司 |
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—— 銷售熱線 ——
18618101725 |
Petaka™ G3 cell culture devices / bioreactors can be used to grow aggregates of cells forming pseudo-tissues from any kind of differentiated cells or stem cells. These agregates , can be implanted into an organisms for regenerative research, cancer research and regenerative therapy experimentation
Differentiated cells and tumor cells forming 3D structures provide important experimental information about cell behavior in real tissues.
Microgravity 3D cultures can be obtained in Petaka™ bioreactor when this is subjected to a controlled and adaptive rotation, keeping the cells in microgravity conditions that induces the formation of 3D cell clusters (pseudo-tissues).
Cystic 3D cell culture
This system is suitable to investigate intercellular interactions through soluble mediators, using the simple and unique bioreactor Petaka G3. Conceptually this bioreactor allows:
1) Growth of more than one cell type, separated by an inter-space full of media.
2) Growth of several million cells, letting repeated cell sampling operations without interruption of the culture.
3) Separate sampling of the different cell types included in the co-culture.
4) Repeated media renewal and sampling operations without the interruption of the culture.
The biological product of this concept is the “Cystic 3D cell culture” that offers, in a single volume, at least three separate sheets of cells, with or without matrixes, growing with well defined borders, surrounding apocket (cyst) with 15 to 25 mL of shared media (see upper figure). The system is a cube like structure with 6 sides or 3 pairs of opposed walls facing each other. In this system the central cavity or cyst receives everything secreted or released by the surrounding cells including metabolites, extracellular organelles, exosomes, shedding microvesicles, apoptotic blebs and others. Cell shits are attached to the walls, uncoated or coated with specific matrix of single molecules such as Laminin, Vitronectin, Collagen, etc. or molecular mixtures such as Matrigel or others, all favoring cell attachment through integrins. One side of the device may be coated with a thick layer of semisolid gel allowing the formation of cell clusters and cell penetration evaluation (invasion assays).
Soluble secretions and particulate elements released by cells forming the walls circulate randomly through the cyst media, like in the blastocysts, ovary follicles and others. Those suspended elements become in contact with the other wall cells dragged by soft and slow convection streams, generated by controlled temperature gradients,. Many physiologic reactions are controlled by soluble molecules and particulate elements, such as exosomes released from living cells
The bottom side layer of cells is preferably influenced by particulate elements, it receives by gravity sedimentation all those elements having density over 1, released by the upper cells; this influence is even more intensive on all cells living on the side of the cyst when exposed to a multiplied g force vector, if the device is subject to a convenient centrifugation.
With an accurate control of hypoxia, close to physiologic DO (about 2 ppm), CO2 control and dehydration control (Petaka G3 conditions), these cultures could evolve for more than 2 weeks, allowing long term inter-cellular interactions. The cyst culture system (Petaka G3 device) allows for free-enzyme cell release of all the cells or samples of each cell type separay, therefore sampling each side independently for microscopy, FACS, PCR or biochemistry analysis.
細(xì)胞培養(yǎng)新時代--PetakaG3*秀的細(xì)胞培養(yǎng)裝置！
PetakaG3TM*秀的細(xì)胞培養(yǎng)裝置！
Celartia公司開發(fā)的PetakaG3TM新型細(xì)胞培養(yǎng)裝置，利用可視化的全密封技術(shù)，降低了污染的風(fēng)險；*的氣體交換阻隔系統(tǒng)，使其不需要CO2和濕度控制即可實現(xiàn)細(xì)胞健康生長。該培養(yǎng)裝置還優(yōu)化培養(yǎng)基/細(xì)胞產(chǎn)量比值和生長因子需求，并實現(xiàn)了在同一塊培養(yǎng)板裝置里細(xì)胞的培養(yǎng)和收集，操作簡便，功能多樣。
特點：
* 可使培養(yǎng)器內(nèi)的O2濃度維持在狀態(tài)（不會供應(yīng)過量的O2）
* 無須CO2（可在CO2培養(yǎng)箱內(nèi)培養(yǎng)）
* 無須加濕（幾乎不受外部濕度影響）
* 安全，不必?fù)?dān)心污染（無蓋、不開放、無泄漏）
* 可進(jìn)行長時間的細(xì)胞觀察
* 堅固（沒有容易損壞的薄膜）
* 更適合于細(xì)胞長途安全運(yùn)輸（無須冰凍、干冰運(yùn)輸）
* 添加了的培養(yǎng)基和成長因子，約20ml～24ml培養(yǎng)基提供 150cm2成長面
* 提供約為體積15倍的細(xì)胞培養(yǎng)面
* 增殖的細(xì)胞可長期放置，并維持休眠狀態(tài)（只需放于室溫條件下）
Petaka培養(yǎng)板的應(yīng)用：
* 普通細(xì)胞的培養(yǎng)：使用zui少量的培養(yǎng)基，獲得更多的細(xì)胞
* 低氧細(xì)胞培養(yǎng)：無需特殊的培養(yǎng)箱或手套箱，即可實現(xiàn)低氧細(xì)胞培養(yǎng)
* 細(xì)胞長距離運(yùn)輸：安全、高效的長距離細(xì)胞運(yùn)輸?shù)?選擇
* 細(xì)胞的休眠與復(fù)蘇：對細(xì)胞生物學(xué)、毒性學(xué)無影響
* 細(xì)胞存儲：使得細(xì)胞存儲變得很便利
* 細(xì)胞庫管理：細(xì)胞生長，管理和運(yùn)輸變得簡單
貨號 | 品名 | 包裝 | 品牌 | 用途 |
PET025 | petaka G3 Cell Culture Device Pack LOT | 25塊 | Celartia | 動物細(xì)胞培養(yǎng) |
PET300 | petaka G3 Cell Culture Device Box（12*25 Pieces）LOT | 12*25塊 | Celartia | 動物細(xì)胞培養(yǎng) |
PET120 | petaka G3 Cell Culture Device Box（48*25 Pieces）LOT | 48*25塊 | Celartia | 動物細(xì)胞培養(yǎng) |
PET025H | petaka G3 Cell Culture Device Pack HOT | 25塊 | Celartia | 昆蟲細(xì)胞培養(yǎng) |
PET300H | petaka G3 Cell Culture Device Box（12*25 Pieces） HOT | 12*25塊 | Celartia | 昆蟲細(xì)胞培養(yǎng) |
PET120H | petaka G3 Cell Culture Device Box（48*25 Pieces） HOT | 48*25塊 | Celartia | 昆蟲細(xì)胞培養(yǎng) |
PET025F | petaka G3 flat Cell Culture Device Pack | 25塊 | Celartia | 懸浮細(xì)胞培養(yǎng) |
PET300F | petaka G3 flat Cell Culture Device Box（12*25 Pieces） | 12*25塊 | Celartia | 懸浮細(xì)胞培養(yǎng) |
PET120F | petaka G3 flat Cell Culture Device Box（48*25 Pieces） | 48*25塊 | Celartia | 懸浮細(xì)胞培養(yǎng) |
PET025ET | petaka EF LOT Cell Culture Device Pack | 25塊 | Celartia | *回收細(xì)胞 |
PET300ET | petaka EF LOT Cell Culture Device Box（12*25 Pieces） | 12*25塊 | Celartia | *回收細(xì)胞 |
PET120ET | petaka EF LOT Cell Culture Device Box（48*25 Pieces） | 48*25塊 | Celartia | *回收細(xì)胞 |
PET025ETH | petaka EF HOT Cell Culture Device Pack | 25塊 | Celartia | *回收細(xì)胞 |
PET300ETH | petaka EF HOT Cell Culture Device Box（12*25 Pieces） | 12*25塊 | Celartia | *回收細(xì)胞 |
PET120ETH | petaka EF HOT Cell Culture Device Box（48*25 Pieces） | 48*25塊 | Celartia | *回收細(xì)胞 |
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PETST6 | petaka G3 Stands STANDS Box紅 藍(lán) 橙 紫 綠 黃6色 | 12快 | Celartia | 細(xì)胞培養(yǎng)架子 |
TIP120 | petaka G3 Tip Blunt 18 Guage Box 頓口針頭 | 120支 | Celartia | 進(jìn)樣用 |
PET010 | petaka Transporter Envelopes 運(yùn)輸信封 | 10只 | Celartia | 細(xì)胞運(yùn)輸用 |
TRAIN02 | petaka G3 LOT Training kit contains 50 petakas 6 stands & 120 tips | kit | Celartia | 初次實驗人員套裝 |
TRAIN01 | petaka G3 HOT Training kit contains 50 petakas 6 stands & 120 tips | kit | Celartia | 初次實驗人員套裝 |
TRAIN01ET | petaka ET LOT Training kit 300x petakaET 1x Clearcell 360x tips | kit | Celartia | 初次實驗人員套裝 |
TRAIN02ET | petaka ET HOT Training kit 300x petakaET 1x Clearcell 360x tips | kit | Celartia | 初次實驗人員套裝 |
CCLER1 | Clearcell magnetic field generator | 臺 | Celartia | *回收細(xì)胞 |
CENT21 | DIGICEN21 CENTRIFUGE 離心機(jī) | 臺 | Celartia | 細(xì)胞離心 |
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