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deGlycIT spin columns
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deGlycIT spin columns
deGlycIT microspin columns allow for easy and quick deglycosylation of IgG. deGlycIT microspin columns comes prefilled with IgGZERO® enzyme immobilized on agarose. deGlycIT microspin columns are designed for deglycosylation of up to 0.5 mg IgG in less than 30 minutes.
| Step | Description | |
| Remove lid. Centrifuge 200xg for 1 minute to remove the preservative liquid. Add 100 uL of solution containing your IgG. | ||
| Cap the spin column and incubate for 15 minutes at room temperature | ||
| Put the spin column in a microcentrifuge tube and remove the lid. Collect the solution containing your antibodies by centrifugation 1000xg for 1 minute. |
Results and compatibility of deGlycIT spin column
| SDS page （reducing condition） of human monoclona IgG （Herceptin®） before and after deglycosylation using deGlycIT microspin column. |
InstructionsInstructions deGlycIT microspin | Tech notes |
| 品名 | 貨號(hào) | 規(guī)格 | 報(bào)價(jià) | |
| deGlycIT MicroSpin, cleaves up to 0,5 mg IgG | A0-IZ6-010 | 2 columns | 5210元 | |
| deGlycIT MicroSpin, cleaves up to 0,5 mg IgG | A0-IZ6-025 | 5 columns | 11980元 | |
| deGlycIT MicroSpin, cleaves up to 0,5 mg IgG | A0-IZ6-050 | 10 columns | 20110 | |
| deGlycIT MidiSpin, cleaves 1-10mg | A0-IZ6-100 | 1 column | 16210元 | |
| deGlycIT MaxiSpin, cleaves 10-100mg | A0-IZ6-1000 | 1 column | 47800元 |