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The EpiQuik™ Acetyl-Histone H4 ChIP Kit is a convenient package of tools that allows the experimenter to investigate interactions of histone H4 acetylation and DNA in vivo efficiently. The entire procedure can be completed within 5 hours and produces far more superior results than any competitor kits. The EpiQuik™ Acetyl-Histone H4 ChIP Kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR, MS-PCR, DNA sequencing, and southern blot, as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ ACETYL-HISTONE H4 ChIP KIT?

• The fastest procedure available, which can be finished within 5 hours.
• Strip microwell format makes the assay flexible: manual or high throughput.
• Columns for DNA purification are included: save time and reduce labor.
• Compatible with all DNA amplification-based approaches.
• Simple, reliable, and consistent assay conditions.

The EpiQuik™ Acetyl-Histone H4 ChIP Kit contains all reagents required for carrying out a successful chromatin immunoprecipitation for acetyl-histone H4 from mammalian cells. Particularly, this kit includes a ChIP-grade acetyl-histone H4 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted, sheared, and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured acetyl-histone H4protein-DNA complex, reversed, and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

 

SCHEMATIC PROCEDURE:

CP1 （Wash Buffer）
CP2 （Antibody Buffer）
CP3A （Pre-Lysis Buffer）
CP3B （Lysis Buffer）
CP4 （ChIP Dilution Buffer）
CP5 （DNA Release Buffer）
CP6 （Reverse Buffer）
CP7 （Binding Buffer）
CP8 （Elution Buffer）
Protease Inhibitor Cocktail （100X）*
Proteinase K （10 mg/ml）*
Normal Mouse IgG （0.5 mg/ml）*
Anti-Acetyl-Histone H4 （1 mg/ml）*
8-Well Assay Strips （with Frame）
8-Well Strip Caps
F-Spin Column
F-Collection Tube
User Guide

* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.

[User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]