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          世聯(lián)博研(北京)科技有限公司>供求商機(jī)>OP-0022,OP-0022-100,*,EpiQuik核蛋白提取試劑盒II,EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
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          • OP-0022,OP-0022-100,*,EpiQuik核蛋白提取試劑盒II,EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)

          舉報(bào)
          貨物所在地: 北京北京市
          產(chǎn)地: 美國(guó)epigentek
          更新時(shí)間: 2024-09-21 21:00:07
          期: 2024年9月21日--2025年3月21日
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          產(chǎn)品簡(jiǎn)介

          EpiQuik核蛋白提取試劑盒II
          EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)
          *
          100 assays/2002元 (貨號(hào):OP-0022-100)

          品牌:美國(guó)epigentek

          詳細(xì)介紹

          OP-0022,

          EpiQuik核蛋白提取試劑盒II

           

          EpiQuik Nuclear Extraction Kit II (Nucleic Acid-Free)

          *


           
          100 assays/1990元 (貨號(hào):OP-0022-100)
           
           
           
          品牌:美國(guó)epigentek

           

          背景資料 EpiQuik核蛋白提取試劑盒II(用于無(wú)核酸的核蛋白提?。┨峁┝艘环N簡(jiǎn)單而應(yīng)用廣泛的方法以及相應(yīng)的試劑提取各種用途的核蛋白,整個(gè)過(guò)程耗時(shí)不超過(guò)60分鐘。提取的核蛋白可用于:western印跡、蛋白-DNA結(jié)合分析實(shí)驗(yàn)、核酶分析實(shí)驗(yàn)以及各種其它需要活性核蛋白或者無(wú)核酸核蛋白的實(shí)驗(yàn)。提取實(shí)驗(yàn)迅速簡(jiǎn)單,可以從哺乳動(dòng)物細(xì)胞和組織樣品中分離出大量核提取產(chǎn)物。本試劑盒尤其為滿足各種EpiQuik分析實(shí)驗(yàn)需求而設(shè)計(jì)。試劑盒可以用于提取哺乳動(dòng)物細(xì)胞和組織樣品中的核蛋白。本試劑盒里有kit I和kit II,前者用來(lái)進(jìn)行常規(guī)提取,后者用來(lái)進(jìn)行無(wú)核酸蛋白的提取。
           
          產(chǎn)品描述 EpiQuik核蛋白提取試劑盒II只需要用本公司的去核酸蛋白分離緩沖液加到細(xì)胞/組織樣品。先后加入細(xì)胞預(yù)溶解劑、細(xì)胞溶解劑以及核酸降解溶液,不含核酸的核蛋白即可被提取出來(lái)并立即用于隨后的各種實(shí)驗(yàn)分析或在適當(dāng)條件下儲(chǔ)存起來(lái)。該核蛋白提取試劑盒II(無(wú)核酸)內(nèi)主要產(chǎn)品組份包括:NP1 (10X Pre-Extraction Buffer) NP2 (Extraction Buffer) NP3 (Extraction Pre-Cleaner) NP4 (Extraction Cleaner) 1000X DTT Solution 1000X Protease Inhibitor Cocktail (PIC)用戶指南手冊(cè)
          產(chǎn)品特點(diǎn) 1·操作十分快捷,1小時(shí)內(nèi)可完成;2·應(yīng)用廣泛,可用于多種樣本的核蛋白提取
          保存建議 在接收到Epigentek試劑盒后請(qǐng)按照說(shuō)明書(shū)建議,使用不同的保存條件或溫度來(lái)保存試劑盒內(nèi)組分

          其他 Epigentek集團(tuán)公司是的創(chuàng)新技術(shù)和表觀遺傳學(xué)相關(guān)研究產(chǎn)品的開(kāi)發(fā)商和供應(yīng)商。公司開(kāi)發(fā)了包括400多個(gè)產(chǎn)品的全面的產(chǎn)品組合,為表觀遺傳學(xué)方面的研究和新藥研發(fā)提供全面系統(tǒng)的解決方案。作為多家的代理,世聯(lián)博研與Epigentek一起為研究者們提供、的表觀遺傳學(xué)研究工具而不斷擴(kuò)大產(chǎn)品及服務(wù)質(zhì)量。
           
          Product Overview


           

          (For Nucleic Acid-Free Extraction)
          The EpiQuik™ Nuclear Extraction Kit provides the simple and selective method for extracting nuclear proteins used for a variety of applications. These applications may include western blotting, protein-DNA binding assays, nuclear enzyme assays, and the others requiring optimized or nucleic acid-free nuclear proteins. The EpiQuik™ Nuclear Extraction Kits are also specifically designed to meet the requirements of nuclear extracts used in various EpiQuik™ assays. The EpiQuik™ Nuclear Extraction Kits can be used to extract nuclear proteins from mammalian cells and tissue samples. The EpiQuik™ Nuclear Extraction Kits include kit I for regular nuclear extraction and kit II for nucleic acid-free nuclear extraction. The EpiQuik™ Nuclear Extraction Kits have the fastest procedure that can be finished within 60 minutes.

           

          Note: This item was formerly catalog no. OP-0002-2 (OP00022, OP-00022, OP0002-2)

           

           

           

          Product Citations

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          Babu, PV. et. al. (September 2012). Epigallocatechin gallate reduces vascular inflammation in db/db mice possibly through an NF-?B-mediated mechanism. Mol Nutr Food Res. 56(9):1424-32. PubMed Abstract

          Zopf, S. et. al. (September 2012). Inhibition of DNA methyltransferase activity and expression by treatment with the pan-deacetylase inhibitor panobinostat in hepatocellular carcinoma cell lines. BMC Cancer. PDF

          Ramani, K. et. al. (August 2012). Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis. J. Nutr. Epub ahead of print. PubMed Abstract

          Samudio-Ruiz, SL. et. al. (March 2012). Increased DNA methyltransferase activity and DNA methylation following Epidermal Growth Factor stimulation in ovarian cancer cells. Epigenetics. 7(3):216-24. PubMed Abstract

          Maeda, R. et. al. (2012). Immunostimulatory Activity of Polysaccharides Isolated from Caulerpa lentillifera on Macrophage Cells. Biosci Biotechnol Biochem. 76(3):501-5. PubMed Abstract

          Huang, FY. et. al. (March 2012). Helicobacter pylori induces promoter methylation of E-cadherin via interleukin-1ß activation of nitric oxide production in gastric cancer cells. Cancer. Epub ahead of print. PubMed Abstract

          Neri, F. et. al. (February 2012). Myc Regulates the Transcription of the PRC2 Gene To Control the Expression of Developmental Genes in Embryonic Stem Cells. Mol Cell Biol. 32(4):840-51. PubMed Abstract

          Horiuchi A. et. al. (January 2012). Hypoxia upregulates ovarian cancer invasiveness via the binding of HIF-1α to a hypoxia-induced, methylation free hypoxia response element (HRE) of S100A4 gene. Int J Cancer. Epub ahead of print. PubMed Abstract

          Galoian, K. et. al. (January 2012). mTORC1 inhibition and ECM-cell adhesion-independent drug resistance via PI3K-AKT and PI3K-RAS-MAPK feedback loops. Tumour Biol. Epub ahead of print. PubMed Abstract

          Begum, G. et. al. (January 2012). Epigenetic changes in fetal hypothalamic energy regulating pathways are associated with maternal undernutrition and twinning. FASEB J. Epub ahead of print. PubMed Abstract

          Shu, L. et. al. (December 2011). Epigenetic CpG Demethylation of the Promoter and Reactivation of the Expression of Neurog1 by Curcumin in Prostate LNCaP Cells. AAPS J. 13(4):606-14. PubMed Abstract

          Trisciuoglio, D. et. al. (November 2011). CPTH6, a thiazole-derivative, induces histone hypoacetylation and apoptosis in human leukemia cells. Clin Cancer Res. Epub ahead of print. PubMed Abstract

          Yang, J. et. al. (November 2011). Stem Cell Gene SALL4 Suppresses Transcription through Recruitment of DNA Methyltransferases. J Biol Chem. Epub ahead of print. PubMed Abstract

          Huang, L. et. al. (August 2011). Prevention of transcriptional silencing by a replicator-binding complex consisting of SWI/SNF, MeCP1, and hnRNP C1/C2. Mol Cell Biol. 31(16):3472-84. PubMed Abstract

          Cai, H. et. al. (August 2011). Inhibition of melanoma cell proliferation by targeting Wnt/ß-catenin pathway through Sox4 RNA interference. 31(4):656-9. PubMed Abstract

          Yamawaki, Y. et. al. (August 2011). Antidepressant-like effect of sodium butyrate (HDAC inhibitor) and its molecular mechanism of action in the rat hippocampus. World J Biol Psychiatry. Epub ahead of print. PubMed Abstract

          Geyer, K. et. al. (August 2011). Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424. PubMed Abstract

          Fritzenwanger, M. et. al. (2011). Impact of short-term systemic hypoxia on phagocytosis, cytokine production, and transcription factor activation in peripheral blood cells. Mediators Inflamm. 2011:429501. PubMed Abstract

          Nandakumar, V. et. al. (April 2011). (-)-Epigallocatechin-3-gallate reactivates silenced tumor suppressor genes, Cip1/p21 and p16INK4a, by reducing DNA methylation and increasing histones acetylation in human skin cancer cells. Carcinogenesis. 32(4): 537-44. PubMed Abstract

          Nocchi, L. et. al. (April 2011). Thrombomodulin Is Silenced in Malignant Mesothelioma by a Poly(ADP-ribose) Polymerase-1-mediated Epigenetic Mechanism. J Biol Chem. 286(22): 19478-88. Full PDF Article

          Shi, Y. Y. et. al. (April 2011). Diet and Cell Size Both Affect Queen-Worker Differentiation through DNA Methylation in Honey Bees (Apis mellifera, Apidae). PLoS One. 6(4): e18808. PubMed Abstract

          Jin, Y. et. al. (March 2011). Homocysteine levels impact directly on epigenetic reprogramming in astrocytes. Neurochem Int. Epub ahead of print. PubMed Abstract

          Kawakami, K. et. al. (January 2011). Long interspersed nuclear element-1 hypomethylation is a potential biomarker for the prediction of response to oral fluoropyrimidines in microsalite stable and CpG island methylator phenotype-negative colorectal cancer. Cancer Science. 102(1): 166-74. PubMed Abstract

          Herrera, F. et. al. (December 2010). Synergistic effect of retinoic acid and cytokines on the regulation of glial fibrillary acidic protein expression. J Biol Chem. 285(50): 38915-22. Full PDF Article

          Dusio, G. F. et. al. (November 2010). Stimulation of TLRs by LMW-HA induces self-defense mechanisms in vaginal epithelium. Immunol Cell Biol. Epub ahead of print. PubMed Abstract

          Skowronski, K. et. al. (August 2010).Ischemia dysregulates DNA methyltransferases and p16INK4a methylation in human colorectal cancer cells. Epigenetics. 5(6):547-56. PubMed Abstract

          Rugg-Gunn, PJ. et. al. (June 2010). Distinct histone modifications in stem cell lines and tissue lineages from the early mouse embryo. Proc Natl Acad Sci USA. 107(24):10783-90. PubMed Abstract

          Chen, F. et. al. (June 2010). Influence of silencing TRAF6 with shRNA on LPS/TLR4 signaling in vitro. Journal of Huazhong University of Science and Technology. 30(3): 278-84. PubMed Abstract

          Pandey, M. et. al. (June 2010). Promoter demethylation and chromatin remodeling by green tea polyphenols leads to re-expression of GSTP1 in human prostate cancer cells. International Journal of Cancer. 126(11): 2520-33. PubMed Abstract

          Hu, B. et. al. (May 2010). Epigenetic Regulation of Myofibroblast Differentiation by DNA Methylation. Am J Pathol. 177(1): 21-8. PubMed Abstract

          Kawada, S. et. al. (May 2010). Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation. Am J Physiol Regul Integr Comp Physiol. 299(2): R694-701. PubMed Abstract

          Kastl, L. et. al. (April 2010). Effects of decitabine on the expression of selected endogenous control genes in human breast cancer cells. Mol Cell Probes. 24(2): 87-92. PubMed Abstract

          Nishihara, M. et. al. (February 2010). Transcriptional regulation of the human establishment of cohesion 1 homolog 2 gene. Biochem Biophys Res Commun. 393(1): 111-7. PubMed Abstract

          Perisic, T. et. al. (February 2010). Valproate and amitriptyline exert common and divergent influences on global and gene promoter-specific chromatin modifications in rat primary astrocytes. Neuropsychopharmacology. 35(3): 792-805. PubMed Abstract

          Duthie, S. J. et. al. (January 2010). Folate deficiency alters hepatic and colon MGMT and OGG-1 DNA repair protein expression in rats but has no effect on genome-wide DNA methylation. Cancer Prev Res (Phila, Pa). 3(1): 92-100. PubMed Abstract

          Majid, S. et. al. (January 2010). Genistein reverses hypermethylation and induces active histone modifications in tumor suppressor gene B-Cell translocation gene 3 in prostate cancer. Cancer. 116(1): 66-76. PubMed Abstract

          Fritzenwanger, M. et. al. (2009). Cardiotrophin-1 induces tumor necrosis factor alpha synthesis in human peripheral blood mononuclear cells. Mediators Inflamm. 2009: 489802. PubMed Abstract

          Fujishiro, H. et. al. (December 2009). Involvement of DNA hypermethylation in down-regulation of the zinc transporter ZIP8 in cadmium-resistant metallothionein-null cells. Toxicol Appl Pharmacol. 241(2): 195-201. PubMed Abstract

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          Fujishiro, H. et. al. (August 2009). Involvement of DNA hypermethylation in down-regulation of the zinc transporter ZIP8 in cadmium-resistant metallothionein-null cells. Toxicology and Applied Pharmacology. 241(2): 195-201. PubMed Abstract

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          Nakamura, H. et. al. (May 2009). Role of angiotensin II type 1 receptor on retinal vascular leakage in a rat oxygen-induced retinopathy model. Ophthalmic Research. 41(4): 210-5. PubMed Abstract

          Liu, Z. et. al. (May 2009). Modulation of DNA methylation by a sesquiterpene lactone parthenolide. Journal of Pharmacology and Experimental Therapeutics. 329(2): 505-14. PubMed Abstract

          Majid, S. et. al. (April 2009). BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30(4): 662-70. PubMed Abstract

          Wang, X. et. al. (January 2009). RAF may induce cell proliferation through hypermethylation of tumor suppressor gene promoter in gastric epithelial cells. Cancer Science. 100(1): 117-25. Full PDF Article

          Fritzenwanger, M. et. al. (May 2008). Cardiotrophin-1 induces intercellular adhesion molecule-1 expression by nuclear factor κB activation in human umbilical vein endothelial cells. Chin Med J. 121(24): 2592-2598. Full PDF Article

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          Miyazaki, T. et. al. (October 2007). E-cadherin Gene Promoter Hypermethylation in H. pylori-Induced Enlarged Fold Gastritis. Helicobacter. 12(5): 523-531. PubMed Abstract

           

          Product Details


           

          The EpiQuik™ Nuclear Extraction Kit II simply applies our proprietary nucleic acid-free protein isolation buffers to cell/tissues. After treatment with pre-lysis, lysis and nucleic acid eliminating solutions, the nucleic acid-free nuclear proteins are easily extracted for immediate use or storage at proper conditions.

          Product Components


           

          NP1 (10X Pre-Extraction Buffer)
          NP2 (Extraction Buffer)
          NP3 (Extraction Pre-Cleaner)
          NP4 (Extraction Cleaner)
          1000X DTT Solution
          1000X Protease Inhibitor Cocktail (PIC)
          User Guide

          User Guide & MSDS


           

          [User Guide]*
          *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.

          [Material Safety Data Sheet]

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