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世聯(lián)博研（北京）科技有限公司 |
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—— 銷售熱線 ——
18618101725 |
epigentek,P-1001,P-1001,P-1001-1,P-1001-2,*,
產(chǎn)品英文名:Methylamp DNA Modification Kit （40 samples）
產(chǎn)品中文名:DNA甲基化修飾試劑盒（40樣本）,
40 samples/1512元 （貨號(hào):P-1001-1 ）
80 samples/2786元 （貨號(hào):P-1001-2）
品牌：美國epigentek
*
Methylamp DNA Modification Kit
| 背景資料 | Methylamp DNA修飾試劑盒是一套完整實(shí)驗(yàn)組件，它使用Epigentek所*的簡(jiǎn)化流線型亞硫酸氫鹽法，來執(zhí)行DNA甲基化分析。整個(gè)實(shí)驗(yàn)需要1小時(shí)55分鐘，其結(jié)果遠(yuǎn)勝于其競(jìng)爭(zhēng)對(duì)手。本試劑盒能適應(yīng)于MS-PCR，實(shí)時(shí)定量MS-PCR，甲基化測(cè)序和焦磷酸測(cè)序，等同于甲基化的微陣列。 | ||
| 產(chǎn)品描述 | Methylamp DNA修飾試劑盒包含DNA樣本亞硫酸氫鹽轉(zhuǎn)換修飾所需要的全部試劑。DNA與亞硫酸氫鹽試劑反應(yīng)，能特異性的使DNA化學(xué)變性，產(chǎn)生單鏈DNA，從而使胞嘧啶脫氨基形成尿嘧啶。修飾液中含有的*的DNA保護(hù)劑，能在亞硫酸氫鹽處理后阻止化學(xué)性或者嗜熱性降解DNA。無毒性的修飾DNA捕獲液能使DNA緊密的綁定在圓柱過濾器，因此DNA的清洗能有效地去除殘余的亞硫酸氫鈉或者鹽。然后修飾的DNA洗提，儲(chǔ)存在-20度可以保持2個(gè)月。 | ||
| 產(chǎn)品特點(diǎn) | 1、目前市場(chǎng)zui快的操作流程，*的反應(yīng)條件完成實(shí)驗(yàn)僅需要1小時(shí)55分鐘；2、*轉(zhuǎn)換未甲基化的胞嘧啶為尿嘧啶，被修飾的DNA> 99·98%；3、修飾過程中具備zui低的DNA降解，能阻止超過90%的DNA損失；4、啟動(dòng)DNA修飾zui低要求：僅需要50pg或者20個(gè)細(xì)胞；5、非常簡(jiǎn)單，可信賴的實(shí)驗(yàn)結(jié)果和穩(wěn)定的修飾條件。 | ||
| 保存建議 | 在接收到Epigentek試劑盒后請(qǐng)按照說明書建議，使用不同的保存條件或溫度來保存試劑盒內(nèi)組分。 | ||
The Methylamp™ DNA Modification Kit is a complete set of essential components which enables the experimenter to perform DNA methylation analysis using Epigentek's uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within a mere 1 hour and 55 minutes and produces far superior results than any competitor kits. The Methylamp™ DNA Modification Kit is suitable for MS-PCR, real time MS-PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?
The Methylamp™ DNA Modification Kit contains all reagents required for bisulfite conversion on a DNA sample. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to bind tightly to the column filter, thus clean DNA can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
High throughput version also available. [Cat. # P-1008]
Fast DNA Modification version using heating process also available. [Cat. # P-1010]
| SCHEMATIC PROCEDURE: | COMPARATIVE OVERVIEW: |
 |   The different amounts of DNA isolated from a serum sample were chemically modified using the Methylamp™ DNA Modification Kit. Real time PCR was performed by using a pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin. |
| KIT CONTENTS | 40 samples P-1001-1 | 80 samples P-1001-2 |
| R1 （DNA denature） | 0.25 ml | 0.5 ml |
| R2 （DNA modification） | 4 vials | 8 vials |
| R3 （DNA modification） | 5 ml | 10 ml |
| R4 （modified DNA capture） | 14 ml | 28 ml |
| R5 （modified DNA cleaning） | 3 ml | 6 ml |
| R6 （modified DNA elution） | 1 ml | 2 ml |
| F-spin column | 40 | 80 |
| F-collection tube | 40 | 80 |
| User guide | 1 | 1 |
 | [User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name. |
| [Material Safety Data Sheet] |
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