共聚焦顯微鏡觀察軟骨構(gòu)建三維力學(xué)刺激生物反應(yīng)器動(dòng)態(tài)周期性壓應(yīng)力持續(xù)循環(huán)壓力負(fù)荷周期性刺激.
軟骨構(gòu)建三維力學(xué)刺激體外培養(yǎng)多艙生物反應(yīng)器動(dòng)態(tài)周期性壓應(yīng)力持續(xù)循環(huán)壓力負(fù)荷周期性刺激誘導(dǎo)軟骨分化組織工程
C9-X CartiGen生物反應(yīng)器
C9 - X系統(tǒng)被設(shè)計(jì)成一個(gè)多樣品生物反應(yīng)器系統(tǒng)，能夠支持廣泛的細(xì)胞和組織的生長(zhǎng)實(shí)驗(yàn)。反應(yīng)艙zui多可容納9個(gè)圓盤形樣品，zui大的直徑8mm大小x 5mm厚。 C9 - X的*的設(shè)計(jì)集成了內(nèi)室底部多孔壓板允許通過(guò)樣本結(jié)構(gòu)的灌注。此功能可讓研究人員直接通過(guò)結(jié)構(gòu)的流動(dòng)，同時(shí)采用壓縮力刺激?？蛇x功能，如額外的機(jī)械傳感器和灌注系統(tǒng)定制儀器的具體研究中的應(yīng)用。
生物反應(yīng)器可為組織培養(yǎng)物提供不同大小和頻率的壓應(yīng)變以及不同流量的灌流條件,可控精度高、操作簡(jiǎn)單可行、性能穩(wěn)定
See the links below for specific system configurations:
Vascular
HeartValve
Ligament
Cartilage
Bone
Skin
Custom
Cartilage Main
Indexed Cartigen
Cartigen HP
Watch a video of the CartiGen （C10-12） bioreactor.
The DynaGen® Series bioreactors provide mechanical stimulation to three dimensional tissue engineered constructs to create physiological conditions in-vitro. The CartiGen system imparts compressive or hydrostatic stress to a cartilage construct. The stress/strain profiles can be defined by the user and can be a simple harmonic （sinusoidal） or a physiological waveform. Applications include the investigation of cell function and differentiation, pharmaceutical benchtop testing, and the seeding and growth of engineered tissues and medical devices. Researchers are currently utilizing these systems in a wide range of research areas including:
- Stem cell stimulation
- Meniscus regenerative medicine
- Chondrocyte stimulation
The C10-1 system has been designed as a single sample bioreactor system that is able to support a wide range of cell and tissue growth experiments utilizing compression. The chamber can accommodate a single sample with a maximum size of 30 mm diameter x 5 mm thick. Optional features such as additional mechanical transducers customize the instrument for the specific research application.
The C10-12c system has been designed as a multi-sample bioreactor system that is able to support a wide range of cell and tissue growth experiments. This chamber can accommodate up to 12 disc shaped samples with a maximum size of 10mm diameter x 5mm thick. The C10-12c's unique design includes optical grade windows on the bottom of the chamber, allowing researchers to view their samples with a confocol microscope without interrupting the culturing process. Optional features such as additional mechanical transducers customize the instrument for the specific research application.
The C9-x system has been designed as a multi-sample bioreactor system that is able to support a wide range of cell and tissue growth experiments. The chamber can accommodate up to 9 disc shaped samples with a maximum size of 8mm diameter x 5mm thick. The C9-x's unique design integrates porous platens within the bottom of the chamber to permit perfusion through the sample constructs. This feature allows researchers to direct flow through the construct while applying oscillatory compressive stimulation. Optional features such as additional mechanical transducers and perfusion systems customize the instrument for the specific research application.
The Indexed CartiGen （I-Cart） system imparts individualized mechanical compression to 12 cartilage samples. Designed to be placed within a standard incubator, applications include investigating cell function, modulating the growth and development of engineered tissues, or testing drugs and regenerative medicine technologies. For more information on the I-Cart click here.
The HP System has been designed as a single or multi-sample bioreactor hydrostatic pressurization system. The chamber can accommodate up to one disc shaped sample up to 50mm diameter x 5mm thick or up to 6 samples that are 30mm diameter X 5mm thick. This system is unlike the other CartiGen Systems in that stress is applied using a high pressure air source. This enables hydrostatic stresses up to 0.5MPa to be applied. For more information on the CartiGen HP click here.
GrowthWorks® Software & Control:
A fifth generation design, the GrowthWorks Software and Control platform includes advanced capabilities, such as multiple waveform control, data acquisition and multi-motor operation. The intuitive software runs on a Windows® laptop computer and features a simple user interface. User defined stimulation profiles are controlled by the software and are readily monitored using graphical displays. Integrated data acquisition routines capture and record data at user prescribed intervals. The GrowthWorks Software is configurable for running four stimulators while simultaneously monitoring up to 16 transducers, allowing the researcher maximum flexibility.
The GrowthWorks Control Hardware features an embedded In CPU, integrated data acquisition and integrated motor drives. Communications between the laptop and control hardware is transmitted over a standard network cable. Primary advantages of the TGT Laptop/Controller approach are reduced physical size and easy upgradability. As future Windows versions or higher performance Laptops become available, either can be replaced without any software or controller changes. This ensures the GrowthWorks control system will be an integral part of your research for many years to come.
The GrowthApps modules provided added capability to GrowthWorks. The addition of the Stiffness Measurement & Adaptive Control Application enables the system to make real-time measurements of the sample stiffness during culture. Described in U.S. Patent 7,410,792 B2 Instrumented bioreactor with material property measurement capability and process-based adjustment for conditioning tissue engineered medical products., the Application measures the mechanical response of the tissue construct as it is being stimulated and conditioned. The stiffness measurements can be used as guidelines for bench marking progress during culturing or for modifying the stimulation profile. Based upon the measured response, the bioreactor system is then capable of altering the stimulation profile. This technology enables researchers to automatically culture the sample from initial seeding to an implantation-ready state with minimal operator intervention.
- 一、美國(guó)tissuegrowth公司臨床相關(guān)的力學(xué)刺激組織工程生物反應(yīng)器專家，世聯(lián)博研北京科技公司作為美國(guó)tissuegrowth在中國(guó)大陸**代理商，簽約代理以下產(chǎn)品:
- 1、三維力學(xué)加載細(xì)胞組織工程培養(yǎng)系統(tǒng)
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- 2、三維軟骨力學(xué)刺激組織工程生物反應(yīng)器
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- 3、3D血管組織工程搏動(dòng)培養(yǎng)系統(tǒng)
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- 4、3D肌腱韌帶組織工程力學(xué)培養(yǎng)生物反應(yīng)器
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- 5、3D心臟瓣膜組織工程培養(yǎng)系統(tǒng)
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- 6、三維骨應(yīng)力刺激組織工程生物反應(yīng)器
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- 7、三維皮膚力學(xué)機(jī)械刺激組織工程生物反應(yīng)器
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- 8、3D灌注培養(yǎng)組織工程生物反應(yīng)器
- 9、3d支架培養(yǎng)組織工程系統(tǒng)
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- 10、多樣品三維力學(xué)加載細(xì)胞組織組織工程培養(yǎng)系統(tǒng)
- 二、世聯(lián)博研北京科技公司還簽約代理美國(guó)Flexcell公司細(xì)胞拉力、壓力及剪力仿真培養(yǎng)系統(tǒng)
- 是美國(guó)Flexcell在中國(guó)大陸**代理商，簽約代理以下產(chǎn)品:
- 儀器部分:
- 1、FX-5000C細(xì)胞壓力加載系統(tǒng)（flexcell FX5000 Compression system）
- 2、FX-5000T細(xì)胞應(yīng)力加載系統(tǒng)（Flexcell FX5000 Tension system）
- 3、Flex Jr. Tension顯微應(yīng)力加載系統(tǒng)（Flex Jr. Tension）
- 4、TissueTrain三維細(xì)胞組織培養(yǎng)系統(tǒng)（Flexcell Tissue® Train System）
- 5、STR-4000細(xì)胞流體切應(yīng)力系統(tǒng)（Flexcell Fluid Shear Stress Device）
- 6、應(yīng)力/壓力傳導(dǎo)儀（FlexLink® FX-5000TFL/FX-5000CFL Tension or Compression）
- 7、flexcell自動(dòng)掃描設(shè)備和XyFlEX計(jì)算軟件（ScanFlex with XyFlex）
- 8、HiQFlowmate液體控系統(tǒng)（HIQ-4000-A/B/C HiQ Flowmate-Fluid Control System）
- 9、OsciFlow_切應(yīng)力模式控制器
- 10、SFlex_SFlexJr_顯微應(yīng)力加載系一號(hào)（StageFlexer StageFlexer Jr. Microscopy Tension Device）
- 11、FlexFlow顯微切應(yīng)力加載設(shè)備二號(hào)（StageFlexer StageFlexer Microscopy Tension Device）
- 12、STR-4000C 流體剪切系統(tǒng)W/O型電腦（Streamer Fluid Shear System w/o Computer）
- 13、STR-4000D Streamer Fluid Shear Body Only
- 14、Flexcell 儲(chǔ)壓泵（Pressure Reservoir）
- 15、FlexStop真空隔離閥
- flexcell培養(yǎng)耗材部分:
- 1、TTPlate三維細(xì)胞培養(yǎng)板
- 2、BioFlex（flexcell BioFlex培養(yǎng)板）
- 5、UniFlex（flexcell雙向應(yīng)力培養(yǎng)板）
- 6、BioPress壓力加載系統(tǒng)配套使用的BioPress培養(yǎng)板
- 7、細(xì)胞培養(yǎng)載片
- 8、圓形TTCircular三維細(xì)胞培養(yǎng)板
- 9、HI bioFlex培養(yǎng)板
- 10、SFMembrane_硅膠膜
- Flexcell儀器、配件銷售與租賃
三、世聯(lián)博研北京科技公司還簽約美國(guó)ebersmedical 一個(gè)簡(jiǎn)單的機(jī)械性刺激細(xì)胞培養(yǎng)系統(tǒng)，簽約代理以下產(chǎn)品:
- 1、TC-3 Bioreactor（簡(jiǎn)單的機(jī)械性刺激細(xì)胞培養(yǎng)系統(tǒng)）
- A simple mechanical stimulation cell culture system at last!
- The TC-3 Bioreactor has been designed as a simple easy-to-use system suitable for cell culture
- under mechanical loading profiles defined by the user.
- It has been designed and manufactured thinking of the needs of
- researcher working on cell culture under mechanical stimuli.
- The TC-3 combines the features of a traditional testing machine with the particular demands of cell culture,
- with special emphasis on the parts sterilization, easy assembling,
- sample inspection by microscopy techniques and simple manipulation. Also scalability and
- versatility are key features of the TC-3, in which three grips models can be interchanged
- in order to adapt to the features of the testing substrate or scaffold.
- The system is controlled by a simple computer interface which allows defining the most
- common loading profiles which can be applied on the culture substrate.
- 2、TEB1000 flow bioreactor（TEB1000流體生物反應(yīng)器）
- 3、TC-3 mechanical stimulation bioreactor（TC-3機(jī)械性刺激生物反應(yīng)器）
- 4、P3D perfusion chambers（灌注細(xì)胞培養(yǎng)）
- 5、Vascular Chamber（血博灌注細(xì)胞培養(yǎng)）
四、簽約代理美國(guó)B-Bridge細(xì)胞牽拉儀（STREX）
美國(guó)B-Bridge細(xì)胞牽拉儀（STREX）是一種模擬細(xì)胞生長(zhǎng)過(guò)程中的機(jī)械應(yīng)力裝置。通過(guò)施加細(xì)胞應(yīng)力，張力腔室中的種子細(xì)胞被拉伸或壓縮。施加在細(xì)胞上的機(jī)械應(yīng)力更好的模擬了自然動(dòng)態(tài)生理環(huán)境。STREX可控制施加在細(xì)胞上的應(yīng)力級(jí)別，部分型號(hào)可與顯微鏡聯(lián)用。
ST-140-04
ST-140-10
| Key: S=Standard, A=Available, - = not available |
| Individual sample growth chamber | S | S | S | |
| Multi-sample growth chamber | - | S | S | |
| Perfusion and instrumentation ports per chamber | - | - | 9 | |
| Max construct size: 30 mm diameter by 5 mm thick | S | - | - | |
| Max construct size: 10 mm diameter by 5 mm thick | S | S | - | |
| Max construct size: 6 mm diameter by 5 mm thick | S | S | S | |
| Sample visualization via confocal microscopy | - | S | - | |
| | | | | |
| Axial stimulator and reaction frame | S | S | S | |
| Compressed air and manifold | - | - | - | |
| | | | | |
| Controller software, computer, and monitor | S | S | S | |
| Power supplies and cabling | S | S | S | |
| Mean flow control | A | - | S | |
| Dynamic flow control | A | - | S | |
| | | | | |
| Non-contact flow sensors | A | A | A | |
| Axial load cell | S | S | S | |
| Pressure transducer | - | - | - | |
| | | | | |
| Media reservoir | S | - | S | |
| Incubator | A | A | A | |
| | | | | |
| All-autoclavable components | S | S | S | |
| Medium change without opening chamber via luer fittings | - | - | S | |
| Increased sterility with fast and clean sample change and access | S | S | S | |
| Lightweight design for easy handling | S | S | S | |
| Easily cleaned surface finishes | S | S | S | |
| Compact design for fit in standard incubators 6.5cubic ft | S | S | S | |
| * satisfaction guaranteed warranty | S | S | S | |
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