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| Reactivity: | All mammalian species |
| Formulation: | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% （w/v） BSA （origin USA）. |
| Preparation: | The purified protein was conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647. |
| Storage & Handling: | The Annexin V solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. |
| Application: | FC - Quality tested |
| Recommended Usage: | Each lot of this product is quality control tested by staining of apoptotic cells and analyzing by flow cytometry. The suggested use of this reagent is 5 µl per 1X105 cells in a 100 µl volume of Annexin V Binding Buffer （cat. no. 422201）. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm. ** Alexa Fluor® is a registered trademark of Molecular Probes, Inc. Alexa Fluor® dye antibody conjugates are sold under license from Molecular Probes, Inc. for research use only, except for use in combination with microarrays and high content screening, and are covered by pending and issued patents. |