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產(chǎn)品名稱：Invitrogen 15224-025 T4 DNA 連接酶
貨 號：15224-025
規(guī) 格：500U(1U/ul)
分 類： DNA/RNA 修飾酶
品 牌：Invitrogen
國產(chǎn)/進(jìn)口：進(jìn) 口
儲存溫度：-20°C
特惠價(jià)格：致 電
庫存狀態(tài)：現(xiàn) 貨
銷售商：上海慧穎生物科技有限公司
慧穎生物專業(yè)供應(yīng)Invitrogen 15224-025 T4 DNA 連接酶，常備現(xiàn)貨，*。主營Life Invitrogen產(chǎn)品，囊括TRlzol ?Reagent、TRIZOL LS Reagent 、B-27添加劑、凝膠填料、常規(guī)化學(xué)試劑、培養(yǎng)基及原料試劑、各類胎牛血清、馬血清等，如有需要，詳詢。
描述：
T4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3′ hydroxyl and 5′ phosphate termini. The unique T4 DNA Ligase buffer optimizes ligation, which can be performed in 5 minutes. Single-stranded nucleic acids are not substrates for this enzyme.
Applications
Cloning (blunt-end or cohesive-end ligation) and adding linkers or adapters to blunt-ended DNA
Source
Purified from E. coli lambda lysogen NM989
Performance and quality testing
Endodeoxyribonuclease, 3′ and 5′ exodeoxyribonuclease assays; ligation efficiency tested
Unit definition
One unit catalyzes the exchange of 1 nmol 32P-labeled pyrophosphate into ATP in 20 min at 37°C. One unit is equal to approximay 300 cohesive-end ligation units.
Unit reaction conditions
66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl2, 10 mM DTT, 66 μM ATP, 3.3 μM 32P-labeled pyrophosphate, and enzyme in 0.1 mL for 20 min at 37°C.
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