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waters 蛋白質(zhì)反相色譜柱
waters 蛋白質(zhì)反相色譜柱是一種基于蛋白質(zhì)在溶液中的相對疏水性的分離技術(shù),分析時使用裝填有短鏈鍵合相的大孔徑顆粒(例如,300Å)的色譜柱。如果使用離子對試劑(例如0.1% TFA)來zui大限度減少不良離子干擾,通常利用逐漸升高的有機溶劑濃度梯度來控制分離。一般來說,洗脫順序取決于蛋白質(zhì)或蛋白質(zhì)亞基的疏水性,疏水性zui弱的蛋白質(zhì)將首先洗脫出來。此外,顆粒組成(硅膠vs雜化顆粒)、孔徑、鍵合相類型和密度,以及分離條件(例如梯度持續(xù)時間、分離溫度、流速)等等對于實現(xiàn)滿足應(yīng)用要求的分離而言也非常重要。
專為蛋白質(zhì)分離設(shè)計
分離大分子生物化合物的難度較大。20多年來,沃特世反相色譜柱在蛋白質(zhì)分離方面的性能優(yōu)勢為色譜工作者提供了有力支持。面對棘手的蛋白質(zhì)分離難題,沃特世亞乙基橋雜化(BEH)顆粒技術(shù)有助于用戶突破使用*硅膠基質(zhì)填料色譜柱時的性能局限。
150 μm iKey分離裝置的“即插即用”式設(shè)計和無接頭流路連接大大簡化了采用微流LC-MS的蛋白質(zhì)分析方法。與1 mm內(nèi)徑的UPLC相比,iKey將分析靈敏度提高了多達40倍。
CQUITY UPLC M-Class色譜柱專為低擴散納流LC而設(shè)計,適用于納流LC-MS蛋白質(zhì)分析。納升級至微升級LC/MS ACQUITY UPLC M-Class色譜柱和捕集柱可在15000 psi的UPLC條件下進行納升級和微升級分離,充分利用亞2 μm顆粒技術(shù)的分離能力。
BioSuite RPC色譜柱產(chǎn)品中裝填有與pH穩(wěn)定的甲基丙烯酸酯類聚合物樹脂鍵合的苯基(pPhenyl)填料。pPhenyl基質(zhì)的孔徑為1000 Å,可分析分子量達5 000 000道爾頓的蛋白質(zhì)。
pPhenyl RPC填料提供裝填于5 x 150 mm色譜柱的規(guī)格,適用于“實驗室規(guī)模”分離;還提供裝填于2.0 x 75 mm色譜柱的規(guī)格,適用于窄徑HPLC和LC-MS應(yīng)用。
Symmetry300 C4是采用超純有機試劑合成的*硅膠基質(zhì)材料,純度*且硅醇活性極低,對肽和蛋白質(zhì)的分離和回收效果。
Delta-Pak HPLC色譜柱基于高度穩(wěn)定、鍵合、封端的球形5 μm或15 μm 100%硅膠基質(zhì)顆粒。該系列色譜柱有100 Å和300 Å兩種孔徑 - 填充C4或C18鍵合相,可滿足不同的選擇性和保留性需求。提供不同的15 μm小柱和色譜柱配置,能夠?qū)崿F(xiàn)*且可預測的毫克級到克級純化放大。
訂貨信息
貨號 | 描述 |
186007567 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 50 mm, 1/pkg |
186007569 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pkg |
WAT046885 | DeltaPak C4 Guard Column, 300Å, 5 µm, 3.9 mm X 20 mm, 2/pkg |
186007495 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pkg |
WAT011812 | DeltaPak C4 Column, 300Å, 15 µm, 3.9 mm X 300 mm, 1/pkg |
WAT036865 | DeltaPak C4 Guard-Pak Insert, 300Å, 5 µm, 10/pkg |
186007493 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 75 µm X 100 mm, 1/pkg |
186007494 | ACQUITY UPLC M-Class Protein BEH C4 Column, 300Å, 1.7 µm, 100 µm X 100 mm, 1/pkg |
186005589 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 50 mm, 1/pkg |
186004899 | ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 100 mm, 3/pkg |
186006549 | ACQUITY UPLC Protein BEH C4 Method Validation Kit, 300Å, 1.7 µm, 2.1 mm X 150 mm, 3/pkg |
186002156 | BioSuite Phenyl Column, 1000Å, 10 µm, 2 mm X 75 mm, 1/pkg |
186005590 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 100 mm, 1/pkg |
186005591 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 1 mm X 150 mm, 1/pkg |
WAT011794 | DeltaPak C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg |
186004497 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 150 mm, 1/pkg |
186004623 | ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 3/pkg |
186004496 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 100 mm, 1/pkg |
WAT011796 | DeltaPak C4 Column, 100Å, 5 µm, 3.9 mm X 150 mm, 1/pkg |
186004495 | ACQUITY UPLC Protein BEH C4 Column, 300Å, 1.7 µm, 2.1 mm X 50 mm, 1/pkg |
WAT011807 | DeltaPak C4 Column, 100Å, 15 µm, 3.9 mm X 300 mm, 1/pkg |
186002157 | BioSuite Phenyl Column, 1000Å, 10 µm, 4.6 mm X 75 mm, 1/pkg |
WAT046875 | DeltaPak C4 Guard Column, 100Å, 5 µm, 3.9 mm X 20 mm, 2/pkg |
186002158 | BioSuite Phenyl Column, 1000Å, 13 µm, 21.5 mm X 150 mm, 1/pkg |
WAT011813 | DeltaPak C4 Prep Column, 300Å, 15 µm, 7.8 mm X 300 mm, 1/pkg |
WAT011814 | DeltaPak C4 Prep Column, 300Å, 15 µm, 19 mm X 300 mm, 1/pkg |
186000277 | Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 50 mm, 1/pkg |
186000278 | Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 100 mm, 1/pkg |
186000279 | Symmetry C4 Column, 300Å, 3.5 µm, 2.1 mm X 150 mm, 1/pkg |
WAT011810 | DeltaPak C4 Prep Column, 100Å, 15 µm, 30 mm X 300 mm, 1/pkg |
WAT011669 | DeltaPak C4 Radial-Pak Cartridge, 300Å, 15 µm, 47 mm X 300 mm, 1/pkg |
WAT037700 | DeltaPak C4 Radial-Pak Cartridge, 300Å, 15 µm, 40 mm X 100 mm, 1/pkg |
WAT025848 | DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 8.0 mm X 100 mm, 1/pkg |
186000286 | Symmetry C4 Column, 300Å, 5 µm, 3.9 mm X 150 mm, 1/pkg |
186000285 | Symmetry C4 Column, 300Å, 5 µm, 2.1 mm X 150 mm, 1/pkg |
WAT037851 | DeltaPak C4 Radial-Pak Guard, 300Å, 15 µm, 40 mm X 10 mm, 2/pkg |
WAT038524 | DeltaPak C4 Radial-Pak Guard, 100Å, 15 µm, 25 mm X 10 mm, 2/pkg |
186000283 | Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 150 mm, 1/pkg |
WAT038526 | DeltaPak C4 Radial-Pak Guard, 300Å, 15 µm, 25 mm X 10 mm, 2/pkg |
186000282 | Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 100 mm, 1/pkg |
186000281 | Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 75 mm, 1/pkg |
186000280 | Symmetry C4 Column, 300Å, 3.5 µm, 4.6 mm X 50 mm, 1/pkg |
WAT038508 | DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 25 mm X 100 mm, 1/pkg |
WAT034225 | Shodex DE-G Guard Column, 4.6 x 10 mm |
WAT011808 | DeltaPak C4 Prep Column, 100Å, 15 µm, 7.8 mm X 300 mm, 1/pkg |
WAT011809 | DeltaPak C4 Prep Column, 100Å, 15 µm, 19 mm X 300 mm, 1/pkg |
WAT011633 | DeltaPak C4 Radial-Pak Cartridge, 100Å, 15 µm, 47 mm X 300 mm, 1/pkg |